【Objective】Nutrient uptake of soybean is closely related to the yield, while the response of soybean cultivars to nutrient is not the same. A pot experiment was conducted to find out the nutrient uptakes of nitrogen (N), phosphorus (P), potassium (K) of super-high-yield soybean cultivars and the common cultivar under the treatments of different fertilizer rates and planting patterns.【Method】Two super-high-yield soybean cultivars Liaodou 14, Zhonghuang 35, and one common cultivar Liaodou 11 were used in the pot experiment to investigate the effects of different diammonium phosphate(DAP) levels and planting patterns on soybean N, P, K accumulation and distribution in 2012 and 2013. The experiment was laid out in a randomized block experiment design with five replications. DAP was applied at seedling stage. The treatments consisted of three levels of DAP (0, 100 and 200 mg·kg^-1 dry soil) and two planting patterns (single seeding and mixed seeding). At the stages of R2, R6 and R8, five plants were selected randomly for measurements. Seeds and aboveground vegetative samples were collected from all the treatments. The plant samples were dried in the oven at 105℃ for 30 min and 80℃ at least for 72 h to achieve constant weight. The plant samples were weighed, grounded and digested with H₂SO₄-H₂O₂ acid mixture method. Nitrogen content was estimated by Kjeldah(KN520) method. Phosphorus and potassium were determinated by molybdenum stibium anti(UV-2450) and flame photometer (PEAA800), respectively. N, P, K accumulation was calculated by multiplying dry mass with N, P, K percentage contents. The nutrient use efficiency was calculated as grain yield divided by the nutrient amounts of grains. The nutrient harvest index was calculated by dividing the accumulative nutrient amounts of grains with total nutrient amounts produced by the plants. 【Result】The results showed that the uptake, transportation and distribution of N, P, K varied with different cultivars and planting patterns. Super-high-yield cultivars accumulated more nutrients and had a higher nutrient utilization efficiency as well as N, P harvest index than those of common cultivar. The accumulative amounts of N, P, K showed an increased tendency with the increment of DAP levels, especially in the super-high-yield soybean cultivar. N contents increasing range of stem and leaf of super-high-yield cultivar were 66.9% and 30.5% higher than those of common cultivar, respectively, at flowering stage under 200 mg·kg^-1 fertilization level, and the difference range of P contents reached 28.1%. At seed filling stage, the P contents difference of pod wall and seed was increased with fertilization level enhanced, and that the difference range of K contents between varieties reached 18.6% at maturity stage under high fertilization level. The capacity of nutrient uptake and its use efficiency of super-high-yield soybean cultivars were significantly higher than those of common cultivar in fertilization and mixed seeding treatments. N utilization efficiency of super-high-yield cultivar and common cultivar were declined, while P, K harvest index increased in mixed seeding treatment with fertilization level enhanced. Mixed seeding treatment increased the difference of nutrient utilization efficiency and nutrient harvest index between super-high-yield cultivar and common cultivar, which was more obvious at medium (100 mg·kg^-1) and high (200 mg·kg^-1) fertilization levels. N utilization efficiency and P utilization efficiency of super-high-yield cultivar were 13.6% and 14.2%, 2.1% and 10.4% higher than those of common cultivar, respectively, in mixed seeding treatment at medium and high fertilization levels. In addition, the difference range of P utilization efficiency between varieties was increased from 4.9% to 10.8% in mixed seeding treatment. 【Conclusion】In the case of existing interspecies competition between super-high-yield cultivar and common cultivar, the former had a stronger capacity of nutrient uptake and higher transferring efficiency of nutrients under abundant fertilizing amount.