湖羊TGF-β1基因特征、表达及其与排卵数的相关性分析

doi:10.3864/j.issn.0578-1752.2013.21.022

中国农业科学 ›› 2013, Vol. 46 ›› Issue (21): 4586-4593.doi: 10.3864/j.issn.0578-1752.2013.21.022

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

湖羊TGF-β1基因特征、表达及其与排卵数的相关性分析

郭晶1, 李新宇1, 李隐侠2, 潘增祥1, 决肯3, 刘吉英1, 李二林1, 谢庄1, 陈玲4, 李齐发1

1.南京农业大学动物科技学院，南京 210095；
2.江苏省农业科学院畜牧研究所，南京 210014；
3.新疆农业大学动物科学学院，乌鲁木齐 830052；
4.江苏省苏州种羊场，江苏苏州 215000

收稿日期:2013-04-25 出版日期:2013-11-01 发布日期:2013-06-17
通讯作者: 李齐发，E-mail：liqifa@njau.edu.cn
作者简介:郭晶，E-mail：2011105014@njau.edu.cn。李新宇，E-mail：2012105018@njau.edu.cn
基金资助:
国家自然科学基金（30671503）、江苏省自然科学基金（BK2007156）、国家重点基础研究发展计划（2007CB947403）

Characterization, Expression of TGF-β1 Gene and Its Association with Ovulation Rate in Hu Sheep

GUO Jing-1, LI Xin-Yu-1, LI Yin-Xia-2, PAN Zeng-Xiang-1, JUE Ken-3, LIU Ji-Ying-1, LI 二Lin-1, XIE Zhuang-1, CHEN Ling-4, LI Qi-Fa-1

1.College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095;
2.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014;
3.College of Animal Science, Xinjiang Agricultural University, Urumqi 830052;
4.Suzhou Breeding Sheep Farm of Jiangsu Province, Suzhou 215200, Jiangsu

Received:2013-04-25 Online:2013-11-01 Published:2013-06-17

摘要/Abstract

摘要： 【目的】获取湖羊TGF-β1基因序列，了解TGF-β1序列特征和组织表达特征，分析卵巢组织中TGF-β1基因表达水平与排卵数之间的关系。【方法】通过PCR扩增和测序获得湖羊TGF-β1基因序列，并采用生物信息学方法分析其序列特征；采用RT-PCR分析湖羊TGF-β1基因的组织表达特征；采用Real-time qPCR方法分析多羔组与单羔组湖羊卵巢组织TGF-β1基因表达水平的差异，及其与排卵数之间的关系。【结果】湖羊TGF-β1基因编码区序列全长1 173 bp，编码蛋白含有390个氨基酸残基，包含典型的TGF-β前肽和TGF-β like等结构域。TGF-β1基因在湖羊卵巢组织中高表达，多羔组卵巢组织中TGF-β1基因表达水平显著高于单羔组（P＜0.05），且表达水平与排卵数呈极显著正相关（P＜0.01）。【结论】多羔组湖羊卵巢组织TGF-β1基因表达水平显著高于单羔组，且与排卵数间呈极显著正相关，推测TGF-β1基因在湖羊卵泡发育、成熟或排卵过程中发挥重要作用，是影响湖羊高繁殖力的候选基因。

关键词: 湖羊 , TGF-β1基因 , 产羔数 , 排卵数

Abstract: 【Objective】 The present study was performed to clone the TGF-β1 sequence in Hu sheep and to comprehend the sequence character of Hu sheep so as to investigate the relationships between the mRNA level of TGF-β1 gene and ovulation number in ovaries of Hu sheep. 【Method】The sequence of TGF-β1 in Hu sheep was cloned and sequenced, and the sequence was analyzed by bioinformatics. The tissue distributions of TGF-β1 were detected by RT-PCR, and the gene expression level differences in the ovaries between the high- and the low-fecundity groups and the relationships with the ovulation rate were detected by real-time qPCR.【Result】The length of TGF-β1 gene coding sequence was 1 173 bp, encoding 390 amino acids which include some typical domains such as TGF-β propeptide and TGF-β like. RT-PCR assays revealed that TGF-β1 highly expressed in the ovary tissue. The ovarian mRNA expression level of TGF-β1 in high-fecundity group was significantly greater than that in low-fecundity group (P＜0.05), and mRNA expression level was significantly positively correlated with ovulation rate (P＜0.01). 【Conclusion】The ovarian mRNA expression level of TGF-β1 in high-fecundity group was significantly greater than that in low-fecundity group and significantly positively correlated with ovulation rate. These findings indicate that TGF-β1 of Hu sheep play an important role in follicular development, maturation or ovulation, which probably is associated with high fecundity of Hu sheep.

Key words: Hu sheep , TGF-β1 gene , litter size , ovulation rate

郭晶1, 李新宇1, 李隐侠2, 潘增祥1, 决肯3, 刘吉英1, 李二林1, 谢庄1, 陈玲4, 李齐发1. 湖羊TGF-β1基因特征、表达及其与排卵数的相关性分析[J]. 中国农业科学, 2013, 46(21): 4586-4593.

GUO Jing-1, LI Xin-Yu-1, LI Yin-Xia-2, PAN Zeng-Xiang-1, JUE Ken-3, LIU Ji-Ying-1, LI 二Lin-1, XIE Zhuang-1, CHEN Ling-4, LI Qi-Fa-1. Characterization, Expression of TGF-β1 Gene and Its Association with Ovulation Rate in Hu Sheep[J]. Scientia Agricultura Sinica, 2013, 46(21): 4586-4593.

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湖羊TGF-β1基因特征、表达及其与排卵数的相关性分析

Characterization, Expression of TGF-β1 Gene and Its Association with Ovulation Rate in Hu Sheep

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