湖羊不同花纹皮肤组织差异表达基因的筛选

doi:10.3864/j.issn.0578-1752.2013.02.017

摘要/Abstract

摘要： 【目的】旨在了解中国特有的白色羔皮羊品种—湖羊不同花纹及其毛囊形成的分子遗传学机理。【方法】利用安捷伦基因芯片技术筛选初生湖羊大花和小花皮肤组织差异表达基因。【结果】差异表达分析显示，3对大花和小花组分别筛选出1 069、2 071和3 879个差异表达基因，共有137个共同基因；差异基因经GO分类显示，主要参与细胞分化、增殖、凋亡、发育、免疫应答、离子转运等生物学过程；另外，所验证的4个差异表达基因的荧光定量PCR结果与芯片结果基本一致。【结论】筛选出了BMP7、MMP2、SNAI1、SFXN1、CDKNIC、MT3、POU1F1等对湖羊大花、小花性状形成可能具有重要影响的候选基因。

关键词: 湖羊 , 毛囊 , 基因芯片

Abstract: 【Objective】The molecular genetic mechanism of China’s unique white lambskin breed different flowers patterns of Hu sheep and hair follicle formation were studied.【Method】The article focused on screening differentially expressed genes of new born Hu-sheep’s skin tissue by Agilent gene chip.【Result】 Differential expression analysis revealed that 1067, 2071 and 3879 differentially expressed genes could be screened from 3 groups of large flowers and small flowers, and compared the 3 groups it could find that there were 137 genes in common. Differentially expressed genes classified by GO (Gene Ontology) analysis mainly involved in cell differentiation, proliferation, apoptosis, growth, immune response, ion transport etc. biological progresses. In addition, the RT-PCR results of 3 differentially expressed genes were consistent with that of gene chip.【Conclusion】Combined with the related literatures, BMP7, MMP2, SNAI1, SFXN1, CDKNIC, MT3, POU1F1 etc. might have important effects on the formation of large flowers and small flowers and could be regarded as candidate genes, and offered the basic data for the further study on the molecule genetic mechanism of hair follicles’ formation in Hu sheep.

Key words: Hu sheep , hair follicles , microarray

孙伟, 倪蓉, 殷金凤, 丁家桐, 张有法, 陈玲, 吴文忠, 周洪. 湖羊不同花纹皮肤组织差异表达基因的筛选[J]. 中国农业科学, 2013, 46(2): 376-384.

SUN Wei, NI Rong, YIN Jin-Feng, DING Jia-Tong, ZHANG You-Fa, CHEN Ling, WU Wen-Zhong, ZHOU Hong. Screening Differentially Expressed Genes of Skin Tissue of Different Flowers Patterns of Hu Sheep[J]. Scientia Agricultura Sinica, 2013, 46(2): 376-384.

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