病程相关蛋白质在水稻与白叶枯病菌互作过程中的表达

doi:10.3864/j.issn.0578-1752.2013.20.001

摘要/Abstract

摘要： 【目的】了解病程相关（pathogenesis-related，PR）蛋白质在植物防卫体系中的表达模式进而探讨水稻抗病的分子机理。【方法】选取10个前期工作中鉴定的差异转录PR基因，利用免疫印迹（Western blotting，WB）技术检测它们在水稻正常生长和与白叶枯病菌（Xanthomonas oryzae pv. oryzae Xoo）互作过程中的表达丰度变化。【结果】发现随着水稻的生长，Os12g43380、Os12g36830、Os12g36860、Os12g36840、Os02g41670、Os05g35290和Os12g33610的表达逐步增加，一般在开花期达到最高，成熟期有所降低。在白叶枯病抗性基因Xa21介导的水稻-Xoo非亲和互作过程中，检测到Os01g51570、Os01g71680和Os12g36850的表达量上调，Os12g36830、Os12g36840、Os02g41670和Os05g35290的表达量下调，并且发现，在亲和互作和非亲和互作反应中PR蛋白质的变化模式相似。对PR基因上游启动子区进行分析，发现存在与抗病相关的顺式作用元件，其中，ARE、HSE、MBS、TC-rich repeats等元件在抗病相关的PR基因上游出现频率较高。【结论】鉴定了在水稻-Xoo互作过程中发生丰度变化的7个PR蛋白质。

关键词: 水稻 , 病程相关 , 免疫印迹 , 白叶枯病 , 抗性反应

Abstract: 【Objective】The expression profiling and functional characterization of pathogenesis-related (PR) protein will be helpful to understand the mechanism of rice disease resistance.【Method】To better understand the function of PR proteins, ten differentially transcripted PR genes identified in our previous work using whole genome rice microarray were selected. Western blotting (WB) analysis were used to survey the expression profile in normal growth leave and rice-Xanthomonas oryzae pv. oryzae (Xoo) interactions.【Result】The results indicated that the expression of Os12g43380, Os12g36830, Os12g36860, Os12g36840, Os02g41670, Os05g35290 and Os12g33610 increased gradually during leave growth, reached the highest peak at flowering stage and decreased slightly at mature stage. In rice-Xoo interactions, the expression of Os01g51570, Os01g71680 and Os12g36850 were up-regulated and the expression of Os12g36830, Os12g36840, Os02g41670 and Os05g35290 were down-regulated. It is interesting to note that the expression pattern of PR proteins was similar in both compatible and incompatible rice-Xoo interactions. In addition, cis-elements analysis of the promoter region of PR genes revealed possible defense-related cis-elements, ARE, HSE, MBS and TC-rich repeats were found more frequently in the upstream region of defense-related PR genes.【Conclusion】Seven differentially expressed PR proteins in rice-Xoo interactions were identified.

Key words: rice , pathogenesis related (PR) , western blotting , Xanthomonas oryzae pv. oryzae (Xoo) , resistance

关明俐, 窦世娟, 李雪姣, 贾霖, 史佳楠, 曾祥然, 贾盟, 郭美岑, 刘丽娟, 李莉云, 刘国振. 病程相关蛋白质在水稻与白叶枯病菌互作过程中的表达[J]. 中国农业科学, 2013, 46(20): 4179-4188.

GUAN Ming-Li, DOU Shi-Juan, LI Xue-Jiao, JIA Lin, SHI Jia-Nan, ZENG Xiang-Ran, JIA Meng, GUO Mei-Cen, LIU Li-Juan, LI Li-Yun, LIU Guo-Zhen. Expression of Pathogenesis-Related Proteins in the Interactions Between Rice and Xanthomonas oryzae pv. oryzae[J]. Scientia Agricultura Sinica, 2013, 46(20): 4179-4188.

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