两种中药成分对肉鸡脂质代谢的影响

doi:10.3864/j.issn.0578-1752.2013.13.016

摘要/Abstract

摘要： 【目的】探讨日粮中分别添加山楂叶总黄酮、何首乌二苯乙烯苷及二者同时添加对肉鸡屠宰性能、血清生化指标及脂质代谢相关功能基因表达的影响。【方法】选用1日龄AA肉鸡128只（雄），随机分为4个处理组，即空白对照组（饲喂玉米-豆粕型基础日粮）、Ⅰ组（在日粮中添加300 mg•kg-1山楂叶总黄酮）、Ⅱ组（在日粮中添加150 mg•kg-1何首乌二苯乙烯苷）和Ⅲ组（在日粮中添加300 mg•kg-1山楂叶总黄酮和150 mg•kg-1何首乌二苯乙烯苷）。每组4个重复，每个重复8只。【结果】①与对照组相比，Ⅰ组的全净膛率、血清高密度脂蛋白胆固醇、肝脏组织中过氧化物酶增生物激活受体α和肉毒碱棕榈酰基转移酶-Ⅰ的表达量均显著升高（P＜0.05）；腹脂率、血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇、肝脏组织中固醇调节元伴结合蛋白-1、乙酰辅酶A羧化酶、脂肪酸合成酶的表达量均显著降低（P＜0.05）。②与对照组相比，Ⅱ组的屠宰率、血清高密度脂蛋白胆固醇、游离脂肪酸、肝脏组织中过氧化物酶增生物激活受体α和肉毒碱棕榈酰基转移酶-Ⅰ的表达量均显著升高（P＜0.05）；腹脂率、血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇、肝脏组织中固醇调节元伴结合蛋白-1、乙酰辅酶A羧化酶、脂肪酸合成酶的表达量均显著降低（P＜0.05）。③Ⅲ组的屠宰率、半净膛率、全净膛率、胸肌率、腿肌率和皮下脂肪厚均显著高于对照组（P＜0.05）；屠宰率、全净膛率显著高于Ⅰ组、Ⅱ组（P＜0.05）；腿肌率显著高于Ⅰ组（P＜0.05）；腹脂率显著低于对照组、Ⅰ组、Ⅱ组（P＜0.05）。血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇均显著低于对照组、Ⅰ组、Ⅱ组（P＜0.05）；血清高密度脂蛋白胆固醇、游离脂肪酸、总蛋白、白蛋白均显著高于对照组（P＜0.05）；高密度脂蛋白胆固醇、游离脂肪酸显著高于Ⅰ组、Ⅱ组（P＜0.05）；肝脏组织中的过氧化物酶增生物激活受体α、肉毒碱棕榈酰基转移酶-Ⅰ、脂蛋白酯酶表达量与对照组、Ⅰ组、Ⅱ组相比，显著升高（P＜0.05）；固醇调节元伴结合蛋白-1、乙酰辅酶A羧化酶、脂肪酸合成酶表达量与对照组、Ⅰ组、Ⅱ组相比，均显著降低（P＜0.05）。【结论】日粮中单独或同时添加山楂叶总黄酮和何首乌二苯乙烯苷能提高肝脏组织中过氧化物酶增生物激活受体α、肉毒碱棕榈酰基转移酶-Ⅰ、脂蛋白酯酶 mRNA表达，降低肝脏组织中S固醇调节元伴结合蛋白-1、乙酰辅酶A羧化酶、脂肪酸合成酶 mRNA表达，改善血清脂质代谢相关生化指标，提高肉鸡的屠宰性能。且以二者同时添加的效果最好。

关键词: 山楂叶总黄酮 , 何首乌二苯乙烯苷 , 肉鸡 , 屠宰性能 , 生化指标 , 脂质代谢

Abstract: 【Objective】The experiment was conducted to investigate the effects of TFHL, TSG and TFHL+TSG supplement in daily ration for broilers on slaughter performance, serum biochemical index and expressions of lipid metabolism related genes.【Method】One hundred and twenty-eight male broilers (1 day old) were divided randomly into 4 groups:control group (fed a basal corn-soybean meal diet), groupⅠ (basal diet＋300 mg•kg-1 TFHL), groupⅡ (basal diet＋150 mg•kg-1 TSG) and group Ⅲ(basal diet＋300 mg•kg-1 TFHL＋150 mg•kg-1 TSG). Four replicates were designed for each group and each replicate had 8 broilers.【Result】Compared with the control group, the eviscerate percentage, serum HDL-C, mRNA expressions of PPARα, CPT-Ⅰ in liver tissue in groupⅠwere significantly higher (P＜0.05); the abdominal fat percentage, serum TG, TC and LDL-C, mRNA expressions of SREBP-1, ACC and FAS in liver tissue were significantly decreased (P＜0.05). Compared with the control group, the dressing percentage, serum HDL-C and FFA, mRNA expressions of PPARα, CPT-Ⅰ in liver tissue in groupⅡwere significantly higher (P＜0.05). The abdominal fat percentage, serum TG, TC and LDL-C, mRNA expressions of SREBP-1, ACC and FAS in liver tissue were significantly decreased (P＜0.05). Compared with the control group, the dressing percentage, semi-eviscerate percentage, eviscerate percentage, breast muscle percentage, leg muscle percentage and subcutaneous fat thickness in group Ⅲ were significantly higher (P＜0.05). The dressing percentage and eviscerate percentage in group Ⅲ were much higher than those in groupsⅠand Ⅱ (P＜0.05), and the leg muscle percentage was much higher than groupⅠ (P＜0.05). The abdominal fat percentage in group Ⅲ was significantly lower than that of control group, groupsⅠand Ⅱ(P＜0.05). The TG, TC and LDL-C of serum in group Ⅲ were significantly lower than those of the control group, groupsⅠand Ⅱ(P＜0.05).Compared with the control group, the HDL-C, FFA, TP and ALB of serum in group Ⅲ were significantly higher (P＜0.05). The HDL-C and FFA of serum in group Ⅲ were significantly higher than those in groupsⅠand Ⅱ(P＜0.05). Compared with the other groups, mRNA expressions of PPARα, CPT-Ⅰ, LPL in group III were significantly higher (P＜0.05), and the expressions of SREBP-1, ACC and FAS in liver in group III were significantly decreased (P＜0.05).【Conclusion】Supplemental dietary TFHL and TSG could increase the mRNA expressions of PPARα, CPT-Ⅰand LPL, reduce the mRNA expressions of SREBP-1, ACC and FAS, improve the lipid metabolism related biochemical index in serum, raise slaughter performance. Taking all factors into consideration, it is better to add the two ingredients to diet at the same time.

Key words: TFHL , TSG , broiler , slaughter performance , biochemical index , lipid metabolism

张向杰, 孙耀贵, 程佳, 李宏全, 王俊东. 两种中药成分对肉鸡脂质代谢的影响[J]. 中国农业科学, 2013, 46(13): 2788-2795.

ZHANG Xiang-Jie, SUN Yao-Gui, CHENG Jia, LI Hong-Quan, WANG Jun-Dong. Effects of Two Chinese Medicinal Ingredients on Lipid Metabolism in Broilers[J]. Scientia Agricultura Sinica, 2013, 46(13): 2788-2795.

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