南方主要土壤中铁铝氧化物对土壤吸附Bt蛋白的影响

doi:10.3864/j.issn.0578-1752.2012.23.010

摘要/Abstract

摘要： 【目的】研究Bt蛋白与南方主要土壤（砖红壤、赤红壤、红壤和黄壤）的相互作用，探讨不同形态铁铝氧化物对吸附的影响。【方法】采用平衡吸附法研究Bt蛋白在不同土壤上的吸附特征，并用Langmuir方程进行拟合。【结果】Bt蛋白在去游离态铁铝砖红壤和红壤表面吸附曲线为H型，而在其它供试土壤表面的吸附曲线均为L型，可用Langmuir方程拟合；去除络合态铁铝土壤对Bt蛋白吸附量增加，而去除其它形态铁铝氧化物土壤上的吸附量均有不同程度的降低，且游离态铁铝对Bt蛋白吸附的影响最大；Bt蛋白在去除络合态铁铝土壤表面吸附亲和力最大，在去除游离态铁铝的供试土壤表面亲和力最小。【结论】除络合态铁铝外，游离态、非晶形态等铁铝氧化物能促进土壤对Bt蛋白的吸附，这将为Bt蛋白在土壤中环境风险评价提供参考。

关键词: Bt蛋白 , 吸附 , 土壤 , 铁铝氧化物

Abstract: 【Objective】This paper studied the interaction between Bt toxin and several soils in South of China (Latosols, Latosolic red soil, red soil, yellow soil and paddy soil), and explored the effects of Fe and Al oxides on the adsorption. 【Method】 The adsorption characteristics of Bt toxin by different soils were measured by isothermal adsorption method and fitted by Langmuir equation. 【Result】 The results indicated that except the isotherm curve of Bt toxin adsorbed by Latosols and red soil after removal of free Fe and Al oxides were H type, the adsorption isotherms of Bt toxin by other tested soils were all L-shaped and fitted to Langmuir equation. The adsorption amount of Bt toxin by soils after removal organic bound Fe and Al oxides increased compared to their original soils, while the adsorption amount by soils after removal other species of Fe and Al oxides decreased, and the greatest impact was observed after the removal of free Fe and Al oxides. The adsorption affinity (KL) of Bt toxin adsorbed by soils after removal of organic bound Fe and Al oxides was the highest, and the lowest was shown on soils after removal of free Fe and Al oxides.【Conclusion】 Except organic Fe and Al oxides, Fe and Al oxides (such as the free, amorphous Fe and Al oxides, etc.) promoted the adsorption of Bt toxin by soils, which could provide a reference for the assessment of the environmental risk of Bt protein in soil.

Key words: Bt toxin , adsorption , soil , Fe and Al oxides

付庆灵, 邓雅丽, 胡红青, 蔚霞, 韩晓芳, 李妍. 南方主要土壤中铁铝氧化物对土壤吸附Bt蛋白的影响[J]. 中国农业科学, 2012, 45(23): 4836-4843.

FU Qing-Ling, DENG Ya-Li, HU Hong-Qing, WEI Xia, HAN Xiao-Fang, LI Yan. Effects of Fe and Al Oxides on Adsorption of Bt Toxin by Several Soils in South of China[J]. Scientia Agricultura Sinica, 2012, 45(23): 4836-4843.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2012.23.010

https://www.chinaagrisci.com/CN/Y2012/V45/I23/4836

参考文献

[1]James C. Global status of commercialized biotech/GM crops: 2009. ISAAA Brief No. 41. New York: International Service for the Acquisition of Agri-Biotech Applications (ISAAA), 2009.

[2]Stotzky G. Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis and of bacterial DNA bound on clays and humic acids. Journal of Environmental Quality, 2000, 29: 691-705.

[3]Stotzky G. Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis, especially from transgenic plants. Plant and Soil, 2004, 266: 77-89.

[4]Crecchio C, Stotzky G. Biodegradation and insecticidal activity of the toxin from Bacillus thuringiensis subsp. kurstaki bound on complexes of montmorillonite-humic acids-Al hydroxypolymers. Soil Biology and Biochemistry, 2001, 33: 573-581.

[5]孙彩霞, 陈利军, 武志杰. 转Bt基因作物毒素土壤存活特性及与土壤性质的关系研究进展. 应用生态学报, 2002, 13(11): 1478-1482.

Sun C X, Chen L J, Wu Z J. Research advances in soil persistence characteristics of toxins released by transgenic Bt crops and their relationships with soil properties. Chinese Journal of Applied Ecology, 2002, 13(11): 1478-1482. (in Chinese)

[6]李云河, 王桂荣, 吴孔明, 张永军, 原国辉, 郭予元. Bt作物杀虫蛋白在农田土壤中残留动态的研究进展. 应用与环境生物学报, 2005, 11(4): 504-508.

Li Y H, Wang G R, Wu K M, Zhang Y J, Yuan G H, GuoY Y. Progress in persistence dynamics of insecticidal proteins released from Bt crops in cropland. Chinese Journal of Applied and Environmental Biology, 2005, 11(4): 504-508. (in Chinese)

[7]王洪兴, 陈欣, 唐建军, 志水胜好. 释放后的转抗病虫基因作物对土壤生物群落的影响. 生物多样性, 2002,10(2): 232-237.

Wang H X, Chen X, Tang J J, Shimizu katsuyoshi. Influence of released transgenic pest- and disease-resistant crops on plant-associated microorganisms in soil. Biodiversity Science, 2002, 10(2): 232-237. (in Chinese)

[8]Zhou X Y, Huang Q Y, Chen S W, Yu Z N. Adsorption of the insecticidal protein of Bacillus thuringiensis on montmorillonite, kaolinite, silica, goethite and red soil. Applied Clay Science, 2005, 30: 87-93.

[9]Pagel-Wieder S, Niemeyer J, Fischer W R, Gessler F. Effects of physical and chemical protection of soils on adsorption of the insecticidal protein (Cry1Ab) from Bacillus thuringienses at Cry1Ab protein concentrations relevant for experimental field sites. Soil Biology and Biochemistry, 2007, 39: 3034-3042.

[10]Fu Q L, Peng Y W, Huang T, Hu H Q, Deng Y L, Yu X. Effects of ionic strength and sesquioxides on adsorption of the toxin of Bacillus thuringiensis subsp. kurstaki on soils. Pedosphere, 2012, 22(1): 96-102.

[11]Fu Q L, Hu H Q, Chen S W, Huang Q Y, Liang W. Adsorption of the insecticidal protein of Bacillus thuringiensis subsp. kurstaki by some Chinese soils: effects of organic acid ligands addition. Plant and Soil, 2007, 296: 35-41.

[12]Fu Q L, Wang W Q, Hu H Q, Chen S W. Adsorption of the insecticidal protein of Bacillus thuringiensis subsp. kurstaki by minerals: effects of inorganic salts. European Journal of Soil Science, 2008, 59: 216-221.

[13]姚艳玲, 崔海瑞, 卢美贞, 忻雅. 转基因植物释放Bt毒素的土壤环境行为与生物学效应. 土壤学报, 2005, 42(6): 1024-1029.

Yao Y L, Cui H R, Lu M Z, Xin Y. Environmental behavior and biological effects of Bt toxins released from Bt-transgenic plants in soil. Acta Pedologica Sinica, 2005, 42(6): 1024-1029. (in Chinese)

[14]白耀宇, 蒋明星, 程家安, 姜永厚. 转Bt 基因作物Bt 毒蛋白在土壤中的安全性研究. 应用生态学报, 2003, 14(11): 2062-2066.

Bai Y Y, Jiang M X, Cheng J A, Jiang Y Y. Advances in safety studies of soil Bt toxin proteins released from transgenic Bt crops. Chinese Journal of Applied Ecology, 2003, 14(11): 2062- 2066. (in Chinese)

[15]Quiquampoix H. Mechanisms of protein adsorption on surfaces and consequences for extracellular enzyme activity in soil. Soil Biochemistry, 2000, 10: 171-206.

[16]熊毅．土壤胶体. 第二册．北京: 科学出版社, 1985.

Xiong Y. Research Methods of Soil Colloid. Soil Colloids. Vol.2. Beijing: Science Press, 1985. (in Chinese)

[17]Langmuir I. The adsorption of gases on plane surfaces of glass, mica and platinum. Journal of the American Chemical Society, 1918, 40: 1361-1403.

[18]Sepelyak R J, Feldkamp J R, Moody T E, White J L, Hem S L. Adsorption of pepsin by aluminum hydroxide I: Adsorption mechanism. Journal of Pharmaceutical Science, 1984, 73(11): 1514-1517.

[19]Huang Q Y, Jiang M H, Li X Y. Effects of iron and aluminum oxides and kaolinite on adsorption and activity of invertase. Pedosphere, 1998, 8(3): 251-260.

[20]Madliger M, Sander M, Schwarzenbach R P. Adsorption of transgenic insecticidal Cry1Ab protein to SiO2. 2. patch-controlled electrostatic attraction. Environmental Science and Technology, 2010, 44: 8877-8883.

[21]Madliger M, Gasser C A, Schwarzenbach R P, Sander M. Adsorption of transgenic insecticidal Cry1Ab protein to silica particles effects on transport and bioactivity. Environmental Science and Technology, 2011, 45: 4377-4384.

[22]Tapp H, Calamai L, Stotzky G. Adsorption and binding of the insecticidal proteins from Bacillus thuringicnsis subsp. kurstaki and subsp. tecnebrionis on clay minerals. Soil Biology and Biochemistry, 1994, 26: 663-679.

[23]Muchaonyerwa P, Chevallier T, Pantani O L, Nyamugafata P, Mpepereki S, Chenu C. Adsorption of the pesticidal toxin from Bacillus thuringiensis subsp. tenebrionis on tropical soils and their particle-size fractions. Geoderma, 2006, 133: 244-257.

[24]Malekani K, Rice J A, Lin J S. The effect of sequential removal of organic matter on the surface morphology of humin. Soil Science, 1997, 162: 333-342.

[25]刘冬碧, 贺纪正, 刘凡, 李学垣. 中南地区几种土壤的表面电荷特性Ⅳ．氧化铁铝对土壤表面电荷性质的影响. 土壤学报, 2001, 38(1): 123-127.

Liu D B, He J Z, Liu F, Li X Y. Surface charge characteristics of soils in central and southern China IV. The effect of Fe and Al oxides on surface charge characteristics of soils. Acta Pedologica Sinica, 2001, 38(1): 123-127. (in Chinese)