水稻PP2Ac类磷酸酶蛋白质在盐胁迫下的表达

doi:10.3864/j.issn.0578-1752.2012.12.001

摘要/Abstract

摘要： 【目的】了解重要蛋白质的表达模式进而探讨水稻耐盐的分子机理。【方法】采用基于抗体的蛋白质组学策略，用免疫印迹（western blotting）调查了5个PP2Ac类磷酸酶蛋白质在苗期盐胁迫条件下的表达。【结果】发现在耐盐水稻品种兰胜中，OsPP2Ac-4的表达上调，在盐敏感的水稻品种9311中，OsPP2Ac-2、OsPP2Ac-3和OsPP2Ac-5的表达也发生了上调，但OsPP2Ac-4的表达下调。比较2个品种间PP2Ac蛋白质的表达，发现在正常生长条件下，PP2Ac蛋白质的表达没有显著区别且基本保持恒定，其表达变化仅发生在盐胁迫条件下。分析水稻MPSS数据库提供的苗期盐胁迫的转录数据，发现OsPP2Ac-2、OsPP2Ac-3和OsPP2Ac-5在盐胁迫条件下转录水平下调。【结论】发现了4个盐胁迫条件下表达发生变化的PP2Ac蛋白质。

关键词: 水稻, PP2Ac蛋白磷酸酶, 盐胁迫, 免疫印迹

Abstract: 【Objective】The protein expression profiling and the mechanism of rice salt-tolerance were studied.【Method】 The expression patterns of five rice phospatase 2A catalytic subunits (PP2Ac) in seedlings under salt-stressed conditions were surveyed by Western blotting via antibody-based proteomics strategy. 【Result】 The results indicated that the expression of OsPP2Ac-4 protein was up-regulated in salt-tolerant rice variety Lansheng, and the expressions of OsPP2Ac-2, OsPP2Ac-3 and OsPP2Ac-5 were up-regulated in salt-sensitive rice variety 9311, while the expression of OsPP2Ac-4 was down-regulated in 9311. The expression of PP2Ac proteins was stable and in a similar level for Lansheng and 9311 under normal growth conditions, but the abundance variations were occurred only under salt-stressed conditions. Based on the Massively Parallel Signature Sequencing (MPSS) data derived from salt-stressed rice seedlings, it was found that the transcription of OsPP2Ac-2, OsPP2Ac-3 and OsPP2Ac-5 were down-regulated by comparison with control seedlings without salt treatment.【Conclusion】Four salt stress-related rice PP2Ac proteins were identified.

Key words: rice (Oryza sativa L.), PP2Ac phosphatase, salt stress, Western blotting

刘钊, 贾霖, 贾盟, 关明俐, 曹英豪, 刘丽娟, 曹振伟, 李莉云, 刘国振. 水稻PP2Ac类磷酸酶蛋白质在盐胁迫下的表达[J]. 中国农业科学, 2012, 45(12): 2339-2345.

LIU Zhao, JIA Lin, JIA Meng, GUAN Ming-Li, CAO Ying-Hao, LIU Li-Juan, CAO Zhen-Wei, LI Li-Yun, LIU Guo-Zhen. Expression on Profiling of Rice PP2Ac Type Phosphatase Proteins in Seedlings Under Salt-stressed Conditions[J]. Scientia Agricultura Sinica, 2012, 45(12): 2339-2345.

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