中国农业科学 ›› 2012, Vol. 45 ›› Issue (9): 1667-1675.doi: 10.3864/j.issn.0578-1752.2012.09.001

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

小麦转录因子基因W16的功能标记作图和关联分析

 雷梦林, 李昂, 昌小平, 徐兆师, 马有志, 刘惠民, 景蕊莲   

  1. 1.山西大学生物工程学院,太原 030006
    2.中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京 100081
  • 收稿日期:2011-12-30 出版日期:2012-05-01 发布日期:2012-01-13
  • 通讯作者: 通信作者景蕊莲,Tel:010-82105829;E-mail:jingrl@caas.net.cn
  • 作者简介:雷梦林,E-mail:leimenglin35@163.com
  • 基金资助:

    国家“973”计划项目(2010CB125905)、CGIAR挑战计划项目(GCP、G7010.02.01)

Functional Marker Mapping and Association Analysis of Gene W16 in Common Wheat

 LEI  Meng-Lin, LI  Ang, CHANG  Xiao-Ping, XU  Zhao-Shi, MA  You-Zhi, LIU  Hui-Min, JING  Rui-Lian   

  1. 1.山西大学生物工程学院,太原 030006
    2.中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京 100081
  • Received:2011-12-30 Online:2012-05-01 Published:2012-01-13

摘要: 【目的】开发小麦DREB转录因子基因W16的功能标记,进行遗传作图,并结合表型性状关联分析,为利用分子标记进行小麦遗传改良提供依据。【方法】以六倍体普通小麦及其野生近缘种的二倍体和四倍体为材料,克隆W16的DNA序列;根据序列多态性设计分子标记;利用中国春缺四体材料对基因进行染色体定位,用DH群体(旱选10号×鲁麦14)进行精细定位并作图;以154份六倍体普通小麦材料构成的自然群体分析表型性状与基因单倍型的关联特性。【结果】利用中国春缺四体材料先将W16定位在1A染色体上。后利用DH群体将W16定位于染色体1A的CWM517和WMC20标记之间,距左、右标记的遗传距离分别为7.8 cM和19.4 cM。在小麦自然群体中共检测到W16的3种单倍型,分别与单株穗数、穗粒数、每穗小穗数和籽粒饱满度关联。【结论】确定了W16所在的染色体位置,鉴定出HapⅡ为增加单株穗数和籽粒饱满度的优良单倍型,HapⅢ为提高穗粒数的优良单倍型,该基因的功能标记和关联分析结果为小麦分子育种提供了重要信息。

关键词: 普通小麦, W16, 功能标记, 遗传作图, 关联分析

Abstract: 【Objective】 The objective of this study is to develop and map functional markers for W16, a gene of transcription factor DREB (dehydration responsive element binding) family, to analyze the relationship between haplotypes and phenotypic traits, and provide a basis for the genetic improvement by molecular markers in wheat (Triticum aestivum L.). 【Method】 The W16 was cloned from hexaploid wheat, and its diploid and tetraploid wild relative species. Molecular markers were developed according to sequence polymorphisms. Chromosomal location and genetic mapping of W16 were performed by Chinese Spring nulli-tetrasomic lines and a doubled haploid (DH) population (Hanxuan 10 × Lumai 14). The relevance between W16 haplotypes and phenotypic traits was analyzed by using a natural population consisted of 154 historical wheat accessions. 【Result】 The W16 was mapped in the interval between marker CWM517 and WMC20 on chromosome 1A in Chinese Spring nulli-tetrasomic lines and DH population, and the distances were 7.8 cM and 19.4 cM from the left and right flanking markers. In the natural populations, three haplotypes of W16 were detected which were associated with spike per plant, grain per spike, spikelet per spike and grain plumpness. 【Conclusion】 The present research mapped W16 on the chromosomes 1A. HapⅡ is considered as a potential superior haplotype for the improvement of spike per plant and grain plumpness, while HapⅢ is a potential superior haplotype for grain per spike. These functional markers and association analysis results provide important information for the molecular breeding of wheat.

Key words: common wheat, W16, functional marker, genetic mapping, association analysis