陆地棉胞质雄性不育系与保持系线粒体基因组RFLP分析

doi:10.3864/j.issn.0578-1752.2012.02.002

中国农业科学 ›› 2012, Vol. 45 ›› Issue (2): 208-217.doi: 10.3864/j.issn.0578-1752.2012.02.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

陆地棉胞质雄性不育系与保持系线粒体基因组RFLP分析

张晓, 张锐, 史计, 孟志刚, 孙国清, 周焘, 郭三堆

1.中国农业科学院生物技术研究所/国家农作物基因资源与遗传改良重大科学工程，北京 100081
2.长春理工大学生命科学技术学院，长春 130022

收稿日期:2011-07-06 出版日期:2012-01-15 发布日期:2011-11-02
通讯作者: 通信作者郭三堆，Tel/Fax：010-82106140；E-mail：gsdui@mail.caas.net.cn
作者简介:张晓，Tel：010-82106128，Fax：010-82106140
基金资助:
国家自然科学基金资助项目（30771371）

RFLP Analysis of Mitochondrial Genomes Between Cytoplasmic Male Sterile Line and Maintainer Line in Upland Cotton

ZHANG Xiao, ZHANG Rui, SHI Ji, MENG Zhi-Gang, SUN Guo-Qing, ZHOU Tao, GUO San-Dui

1.中国农业科学院生物技术研究所/国家农作物基因资源与遗传改良重大科学工程，北京 100081
2.长春理工大学生命科学技术学院，长春 130022

Received:2011-07-06 Online:2012-01-15 Published:2011-11-02

摘要/Abstract

摘要： 【目的】研究棉花细胞质雄性不育（CMS）系与保持系线粒体基因组的差异，为克隆棉花CMS基因奠定基础。【方法】以atpA, atp6, atp9, ccmB, ccmC, ccmFN1, cob, coxI, coxII, coxIII, matR, nad1bc, nad2, nad4, nad5, nad6, nad7c, nad9, rpl5, rrn18等20个线粒体基因保守序列为探针，利用Southern blot方法对棉花细胞质雄性不育系、保持系线粒体基因组进行RFLP分析。【结果】发现atpA、atp9、ccmB、nad1bc、nad6、nad7c、rrn18等基因在棉花不育系和保持系间存在明显RFLP多态性，其中atpA的差异最明显。【结论】atpA是棉花CMS系和保持系线粒体基因组间的一个明显差异位点。CMS系比保持系缺失一个rrn18拷贝。

关键词: 棉花, 细胞质雄性不育, 线粒体基因组, RFLP, atpA ,

Abstract: 【Objective】 Identification of the differences of mitochondrial genomes between cytoplasmic male sterile (CMS) line and maintainer line of cotton could make a foundation for cloning CMS-associated gene in cotton.【Method】In this study, 20 mitochondrial gene probes, atpA, atp6, atp9, ccmB, ccmC, ccmFN1, cob, coxI, coxII, coxIII, matR, nad1bc, nad2, nad4, nad5, nad6, nad7c, nad9, rpl5, and rrn18, were used to identify the differences between the P30B normal fertile (N-) and the P30A male sterile (S-) cytoplasm with Southern blot method.【Result】Among the 20 genes, atpA, atp9, ccmB, nad1bc, nad6, nad7c and rrn18 revealed restriction fragment length polymorphisms (RFLPs) between N- and S-cytoplasm. The differences in atpA locus were most obvious.【Conclusion】 atpA was an important mitochondrial genomic site that showed an obvious difference between CMS line and maintainer line of cotton. One rrn18 gene copy was deleted in the mitochondrial genome of CMS line.

Key words: cotton (Gossypium hirsutum L.), CMS (cytoplasmic male sterility), mitochondrial genome, RFLP, atpA

张晓, 张锐, 史计, 孟志刚, 孙国清, 周焘, 郭三堆. 陆地棉胞质雄性不育系与保持系线粒体基因组RFLP分析[J]. 中国农业科学, 2012, 45(2): 208-217.

ZHANG Xiao, ZHANG Rui, SHI Ji, MENG Zhi-Gang, SUN Guo-Qing, ZHOU Tao, GUO San-Dui. RFLP Analysis of Mitochondrial Genomes Between Cytoplasmic Male Sterile Line and Maintainer Line in Upland Cotton[J]. Scientia Agricultura Sinica, 2012, 45(2): 208-217.

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陆地棉胞质雄性不育系与保持系线粒体基因组RFLP分析

RFLP Analysis of Mitochondrial Genomes Between Cytoplasmic Male Sterile Line and Maintainer Line in Upland Cotton

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