不同植食性昆虫危害棉花后棉株5种防御基因表达变化

doi:10.3864/j.issn.0578-1752.2011.21.006

摘要/Abstract

摘要： 【目的】了解棉花体内5种防御蛋白基因在棉花防御植食性害虫中的作用。【方法】使用定量PCR分别测定棉铃虫、斜纹夜蛾、甜菜夜蛾危害和机械损伤0（未处理的对照）、6、12、24、48、60 h后，多酚氧化酶（PPO）、叶绿体铜/锌超氧化物歧化酶、ATP合酶β亚基、捕光蛋白复合物Ⅱ、热激蛋白（Hsp70）等5种棉花防御蛋白基因表达量的变化。【结果】在4种危害处理试验中，多酚氧化酶、热激蛋白和叶绿体铜/锌超氧化物歧化酶对昆虫的取食危害应答较强，捕光蛋白复合物Ⅱ对机械损伤的应答较强，ATP合酶β亚基对昆虫的取食危害和机械损伤应答都较强。PPO对棉铃虫危害应答较快，对甜菜夜蛾危害的应答反应较为迟钝，对斜纹夜蛾的危害没有特异的应答。经过上述4种处理，棉花体内Hsp70表达量都有明显的反应，对棉铃虫和甜菜夜蛾危害反应上调较大。ATP合酶β亚基在受到对机械损伤和斜纹夜蛾危害后表达量下调，而在受到棉铃虫和甜菜夜蛾危害后表达量上调。捕光蛋白复合物Ⅱ在受到对机械损伤、甜菜夜蛾和斜纹夜蛾危害后表达量下调，而在受到棉铃虫危害后表达量上调。叶绿体铜/锌超氧化物歧化酶在受到对机械损伤和斜纹夜蛾危害后表达量下调，而在受到棉铃虫和甜菜夜蛾危害后表达量上调，该基因对斜纹夜蛾危害的应答较强，对甜菜夜蛾危害特异性应答较弱。【结论】棉花对于不同昆虫的危害所产生的应答响应不同，具有一定的专一性。

关键词: 棉花, 防御基因, 诱导反应, 荧光定量

Abstract: 【Objective】The objective of this study is to understand the role of 5 defense protein genes defensing the phytophagous pests in cotton.【Method】Using the real-time quantitative PCR, the expression changes of the polyphenol oxidase (PPO), chloroplast Cu/Zn superoxide dismutase, ATP synthase β subunit, light-harvesting protein complex Ⅱ and heat shock protein (Hsp70) genes, which were damaged by Helicoverpa armigera, Spodoptera litura, Spodoptera exigua and mechanical damage for 0 (control), 6, 12, 24, 48, 60 h, respectively, were studied. 【Result】 In the 4 damage treatments, the polyphenol oxidase, heat shock protein and chloroplast Cu/Zn superoxide were stronger in response to insect feeding damage, but the light-harvesting protein complex Ⅱ was more sensitive to the mechanical damage, and ATP synthase β subunit was stronger in response to the insect feeding damage and mechanical damage. PPO responded rapidly to the H. armigera infestation, while the reaction to the S. exigua infestation was rather slowly, and without specific answer to the damage of S. litura. After 4 treatments, Hsp70 expression had a significant change, and it increased larger against the damages caused by H. armigera and S. exigua. ATP synthase β subunit was down-regulated to mechanical damage and S. litura. While after damage caused by H. armigera and S. exigua the expression was up-regulated. The light-harvesting protein complex Ⅱ was down-regulated except the damage caused by H. armigera. Chloroplast Cu/Zn superoxide was down-regulated when damaged by machine and S. litura but it up-regulated to the H. armigera and S. exigua, and it responded stronger to the damage caused by H. armigera than S. exigua.【Conclusion】The response of the cotton to different insect damages was different and with a certain specificity.

Key words: cotton, defense gene, induced responses, quantitative

李静, 张帅, 崔金杰. 不同植食性昆虫危害棉花后棉株5种防御基因表达变化[J]. 中国农业科学, 2011, 44(21): 4377-4384.

LI Jing, ZHANG Shuai, CUI Jin-Jie. Changes of mRNA Expression of Five Defense Genes of Cotton Response to Damages Caused by Different Phytophagous Insects[J]. Scientia Agricultura Sinica, 2011, 44(21): 4377-4384.

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