中国农业科学 ›› 2010, Vol. 43 ›› Issue (15): 3191-3198 .doi: 10.3864/j.issn.0578-1752.2010.15.017

• 园艺 • 上一篇    下一篇

模拟酸雨对茶梅生理生态特性的影响

赵栋,潘远智,邓仕槐,尚鹤,王芳,陈睿

  

  1. (四川农业大学林学院)

  • 收稿日期:2010-03-03 修回日期:2010-04-08 出版日期:2010-08-01 发布日期:2010-08-01
  • 通讯作者: 潘远智

Effects of Simulated Acid Rain on Physiological and Ecological Characteristics of Camellia sasanqua

ZHAO Dong, PAN Yuan-zhi, DENG Shi-huai, SHANG He, WANG Fang, CHEN Rui
  

  1. (四川农业大学林学院)

  • Received:2010-03-03 Revised:2010-04-08 Online:2010-08-01 Published:2010-08-01
  • Contact: PAN Yuan-zhi

摘要:

【目的】探讨不同pH的模拟酸雨对茶梅主要生理生态特性的影响。【方法】采用盆栽方法,研究4种不同pH(2.0、3.0、4.0、5.6)的模拟酸雨对茶梅(Camellia sasanqua)叶片膜损伤、MDA含量、抗氧化物酶活性、叶绿素(Chl)含量以及气体交换参数的影响。【结果】在不同pH酸雨胁迫下,茶梅叶片细胞膜透性和MDA含量均呈逐渐升高的趋势,且二者呈显著正相关;SOD、CAT和POD活性呈先升后降的单峰曲线变化,其中,pH 4.0的酸雨处理SOD及CAT活性最大;pH 3.0处理的POD活性最大;Chl含量随酸雨pH的降低而降低,但降到pH 2.0时突然增加,恢复到与对照相近的水平;Pn、Gs及Tr均呈先升后降的趋势;Ci呈逐渐降低的趋势;而Ls则呈逐渐上升的趋势。【结论】茶梅对酸雨具有较强的抵抗能力,pH 3.0大致可以认为是酸雨对茶梅造成隐形伤害的阀值,因此,可以作为酸雨灾害严重地区园林绿化及植被构建的物种之一。

关键词: 模拟酸雨, 茶梅, 抗性, 抗氧化物酶, 生理生态

Abstract:

【Objective】 The objective of this experiment is to study the effect of simulated acid rain at different pH levels on the physiological and ecological characters of camellia. 【Method】 With pot experiments,this paper studied the effect of simulated acid rain that included 4 different pH values (2.0, 3.0, 4.0, 5.6) on the membrane damage and antioxidant enzyme activity, chlorophyll (Chl) content, and gas exchange parameters of camellia (Camellia sasanqua) seedlings leaves. 【Result】 The results showed that under simulated acid rain of different values stress, the membrane permeability and malondialdehyde (MDA) content of camellia leaves gradually increased, and there was a significant correlation between them. The dismutase (SOD), catalase (CAT) and peroxidase (POD) activities were showed a single-peak curve which was firstly increased and then decreased, and the maximum of SOD and CAT appeared at the pH4.0 point and POD at the pH 3.0. Chl content a gradually decreased with decreasing pH value, and then increased significantly at pH 2.0. The net photosynthetic (Pn), intercellular CO2 concentration (Ci), stomatal conductance (Gs), and transpiration rate (Tr) all showed a trend of increase first and then decrease. The stomatal limitation value (Ls) showed an trend of gradual increase. 【Conclusion】 Results show that camellia has strong resistance to acid rain, pH 3.0 can be used as an invisible critical point of acid rain damage to its leaves. Therefore, camellia can be considered as one of the landscaping and vegetation constructing plants in the acid rain-hit area.

Key words: simulated acid rain, Camellia sasanqua, resistance, antioxidant enzymes, physiology and ecology