节节麦在山东省冬小麦田的扩散蔓延及对甲基二磺隆抗性测定

doi:10.3864/j.issn.0578-1752.2021.05.009

中国农业科学 ›› 2021, Vol. 54 ›› Issue (5): 969-979.doi: 10.3864/j.issn.0578-1752.2021.05.009

节节麦在山东省冬小麦田的扩散蔓延及对甲基二磺隆抗性测定

高兴祥¹(),李健¹(),张帅²,张悦丽¹,房锋¹,李美¹(),柏连阳³(),张双应⁴

¹山东省农业科学院植物保护研究所山东省植物病毒学重点实验室,济南 250100
²全国农业技术推广服务中心,北京 100125
³湖南省农业科学院杂草生物学及安全防控生物学湖南省重点实验室,长沙410125
⁴侨昌现代农业有限公司,山东滨州 256600

收稿日期:2020-04-27 接受日期:2020-06-12 出版日期:2021-03-01 发布日期:2021-03-09
通讯作者: 李美,柏连阳
作者简介:高兴祥,E-mail：xingxiang02@163.com。|李健,E-mail：lijian910@163.cm。
基金资助:
国家重点研发计划(2017YFD0201700);山东省农业科学院农业科技创新工程(CXGC2018E04)

Spread and Resistance Level of Aegilops tauschii to Mesosulfuron- Methyl in Winter Wheat Field of Shandong Province

XingXiang GAO¹(),Jian LI¹(),Shuai ZHANG²,YueLi ZHANG¹,Feng FANG¹,Mei LI¹(),LianYang BAI³(),ShuangYing ZHANG⁴

¹Shandong Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Ji’nan 250100;
²National Agro-Tech Extension and Service Center, Beijing 100125
³Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha 410125
⁴Shandong Qiaochang Modern Agriculture Co. , Ltd, Binzhou 256600, Shandong

Received:2020-04-27 Accepted:2020-06-12 Online:2021-03-01 Published:2021-03-09
Contact: Mei LI,LianYang BAI

摘要/Abstract

摘要：

【背景】节节麦（Aegilops tauschii）是小麦的野生近缘植物,是世界公认的十大恶性杂草之一,可安全有效防除节节麦的除草剂品种很少,目前只有ALS抑制剂类除草剂甲基二磺隆作为苗后茎叶处理剂能够应用,该药剂在小麦田应用多年,部分区域发现效果下降,这可能与抗性有关。【目的】明确山东省冬小麦田节节麦扩散蔓延规律以及对甲基二磺隆的抗性水平和抗性机理,为制定山东省冬小麦田节节麦精准区域防控策略提供理论依据。【方法】分别于2009—2010、2013—2014、2018—2019年3次田间调查节节麦在山东省冬小麦田的分布,分析其扩散蔓延规律,室内采用整株生物测定法测定62个节节麦种群对甲基二磺隆的抗性水平,对中等抗性种群进行靶标基因序列检测。【结果】节节麦在山东省冬小麦田10年间呈现快速增长趋势,分布区域逐渐扩大,相对优势度逐渐增大。2009—2010年度,节节麦主要分布在山东西北平原区、中部山区和北部滨海区,平均相对优势度为3.18,在所有杂草中位列第8位;2013—2014年度,节节麦已在山东西北平原区等5个区域广泛分布,平均相对优势度达到7.33,位列第5位;2018—2019年度,节节麦在山东省全境大面积发生,平均相对优势度为11.38,位列第4位。62个节节麦种群中有20个种群对甲基二磺隆产生一定程度的抗性,这些抗性种群分布在节节麦发生早且用药时间长的西北平原区、西南平洼区和中部山区,其他区域未见抗性种群。其中低抗、中抗种群分别为16个（相对抗性指数在5.00—10.00）、4个（相对抗性指数在10.00—15.00）,分别占种群总数的25.81%和6.45%,最高相对抗性指数达到12.63,无高抗种群。对4个中抗种群进行靶标位点突变测定,未检测到位点突变。【结论】节节麦已在山东省7大种植区域大面积分布,且分布区域和相对优势度逐年提高。未有对甲基二磺隆高抗的节节麦种群产生,但已有32.26%种群产生低等或中等程度的抗性,未检测到靶标位点突变,可能是代谢酶引起的非靶标抗性。生产中应避免过分依赖甲基二磺隆进行节节麦防控,建议推广深翻、土壤封闭和苗后茎叶处理相结合的节节麦综合防控策略,延缓节节麦抗性发展。

关键词: 节节麦, 甲基二磺隆, 山东省, 抗性水平, 扩散蔓延

Abstract:

【Background】Aegilops tauschii, a malignant weed, is a wild relative plant of wheat, which is one of the 10 most malignant weeds recognized in the world. At present, only the ALS inhibitor herbicide mesosulfuron-methyl can be used as treatment agent for post-seedling. The effect of mesosulfuron-methyl was decreased in some areas after years of application in wheat fields, which may be related to resistance. 【Objective】The objective of this study is to clarify the spread law and resistance level of A. tauschii to mesosulfuron-methyl in Shandong Province, and to provide a theoretical basis for the establishment of the precise regional control of A. tauschii in winter wheat field of Shandong Province. 【Method】The distribution and spread of A. tauschii in winter wheat field of Shandong Province was investigated three times in the decade of 2009-2019, and whole-plant dose response experiments were conducted to determine the resistance level of 62 A. tauschii populations collected in Shandong Province to mesosulfuron-methy in glasshouses.【Result】The results on the spread of A. tauschii showed that the distribution area was gradually expanding and the harm degree was more and more serious. In 2009 to 2010, A. tauschii mainly distributed in Shandong northwest plain region, middle mountain region and northern coastal region, with a total relative abundance 3.18; in 2013 to 2014, A. tauschii had been widely distributed in the northwest plain and other 4 regions, with a total relative abundance 7.33; in 2018 to 2019, this weed occurred in a large area throughout Shandong Province, with a total relative abundance 11.38. The results on resistance level showed that 20 populations had a certain degree of resistance to mesosulfuron-methyl, which distributed in Shandong northwest plain region, southwest plain region and middle mountain region, and no resistance population was found in other areas. The low resistance and middle resistance populations were 16 (RI ranged from 5.00 to 10.00) and 4 (RI ranged from 10.00 to 15.00), which accounted for 25.81% and 6.45% of the total population, respectively. The maximum RI was 12.63, and no high resistance population was found. Target site mutations were detected in 4 middle resistance populations, and no site mutation was detected.【Conclusion】The A. tauschii has been distributed all over Shandong Province, and its dominance and harmfulness are increasing year by year. Although there is no high resistance population to mesosulfuron-methyl, but there are 32.26% low or middle resistance populations. No site mutation was found in the target mechanism test. In view of the winter wheat field dominated by A. tauschii, it is not only necessary to rely on mesosulfuron-methyl for control, but also to promote the comprehensive control of A. tauschii combined with deep ploughing and other agricultural measures. The soil-treated herbicide is also a good way to reduce weed resistance.

Key words: Aegilops tauschii, mesosulfuron-methyl, Shandong Province, resistance level, spread and dissemination

高兴祥,李健,张帅,张悦丽,房锋,李美,柏连阳,张双应. 节节麦在山东省冬小麦田的扩散蔓延及对甲基二磺隆抗性测定[J]. 中国农业科学, 2021, 54(5): 969-979.

XingXiang GAO,Jian LI,Shuai ZHANG,YueLi ZHANG,Feng FANG,Mei LI,LianYang BAI,ShuangYing ZHANG. Spread and Resistance Level of Aegilops tauschii to Mesosulfuron- Methyl in Winter Wheat Field of Shandong Province[J]. Scientia Agricultura Sinica, 2021, 54(5): 969-979.

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图/表 5

表1

节节麦种群采集地点信息表"

种植区域 Planting area	种群编号 Population code	采集地点 Collecting site	种植区域 Planting area	种群编号 Population code	采集地点 Collecting site
西北平原区 The northwest plain region	DZ-BMC	德州市夏津县白马城镇 Baimacheng Town, Xiajin County, Dezhou City	中部山区 The middle mountain region	JNA-LS	济南市章丘区龙山镇 Longshan Town, Zhangqiu District, Ji’nan City
	DZ-DJ	德州市宁津县杜集镇 Duji Town, Ningjin County, Dezhou City		JNA-TP	济南市济阳区太平镇 Taiping Town, Jiyang District, Ji’nan City
	DZ-EC	德州市平原县恩城镇 Encheng Town, Pingyuan County, Dezhou City		JNA-XZ	济南市长清区夏张镇 Xiazhang Town, Changqing District, Ji’nan City
	DZ-FS	德州市禹城区房寺镇 Fangsi Town, Yucheng District, Dezhou City		TA-CH	泰安市宁阳县漕河镇 Caohe Town, Ningyang County, Taian City
	DZ-GJ	德州市乐陵县郭家镇 Guojia Town, Laoling County, Dezhou City		TA-DY	泰安市东平县大洋镇 Dayang Town, Dongping County, Taian City
	DZ-HJ	德州市夏津县霍集镇 Huoji Town, Xiajin County, Dezhou City		TA-HJL	泰安市肥城县胡家楼镇 Hujialou Town, Feicheng County, Taian City
	DZ-JMY	德州市武城县甲马营镇 Jiamaying Town, Wucheng County, Dezhou City		TA-MZ	泰安市岱岳区满庄镇 Manzhuang Town, Daiyue District, Taian City
	DZ-M	德州市陵城区糜镇 Mi Town, Lingcheng District, Dezhou City		TA-SZ	泰安市泰山区省庄镇 Shengzhuang Town, Taishan District, Taian City
	DZ-WC	德州市武城县武城镇 Wucheng Town, Wucheng County, Dezhou City		ZB-QL	淄博市临淄区齐陵镇 Qiling Town, Linzi District, Zibo City
	DZ-XSD	德州市夏津县新盛店镇 Xinshengdian Town, Xiajin County, Dezhou City	北部滨海区 The northern coastal region	ZB-QC	淄博市高青县青城镇 Qingcheng Town, Gaoqing County, Zibo City
	LC-DYZ	聊城市冠县定远寨镇 Dingyuanzhai Town, Guan County, Liaocheng City		BZ-TZ	滨州市邹平县台子镇 Taizi Town, Zouping County, Binzhou City
	LC-HY	聊城市东昌府区侯营镇 Houying Town, Dongchangfu District, Liaocheng City		BZ-HJ	滨州市惠民县胡集镇 Huji Town, Huimin County, Binzhou City
	LC-SD	聊城市临清县尚店镇 Shangdian Town, Linqing County, Liaocheng City		BZ-LD	滨州市阳信县劳店镇 Laodian Town, Yangxin County, Binzhou City
	LC-XH	聊城市阳谷县西湖镇 Xihu Town, Yanggu County, Liaocheng City		DY-BS	东营市利津县北宋镇 Beisong Town, Lijin County, Dongying City
	LC-YQH	聊城市高唐县鱼邱湖办事处 Yuqiuhu Office, Gaotang County, Liaocheng City	南部山区 The southern mountain region	LY-GQ	临沂市沂水县高桥镇 Gaoqiao Town, Yishui County, Linyi City
	LC-ZZZ	聊城市高唐县赵寨子镇 Zhaozhaizi Town, Gaotang County, Liaocheng City		LY-LZ	临沂市兰陵县卢柞镇 Luzuo Town, Lanling County, Linyi City
西南平洼区 The southwest plain region	HZ-DK	菏泽市鄄城县董口镇 Dongkou Town, Juancheng County, Heze City		LY-QT	临沂市沂南县青驼镇 Qingtuo Town, Yi’nan County, Linyi City
	HZ-HX	菏泽市牡丹区皇乡镇 Huangxiang Town, Mudan District, Heze City		LY-SL	临沂市郯城县胜利镇 Shengli Town, Tancheng County, Linyi City
	HZ-LH	菏泽市单县莱河镇 Laihe Town, Shan County, Heze City		LY-TS	临沂市平邑县铜石镇 Tongshi Town, Pingyi County, Linyi City
	HZ-SG	菏泽市定陶县冉堌镇 Rangu Town, Dingtao County, Heze City		LY-TY	临沂市费县探义镇 Tanyi Town, Fei County, Linyi City
	HZ-TZ	菏泽市巨野县田庄镇 Tianzhuang Town, Juye County, Heze City		ZZ-ZW	枣庄市滕州市张汪镇 Zhangwang Town, Tengzhou City, Zaozhuang City
	JNI-GC	济宁市汶上县郭仓镇 Guocang Town, Wenshang County, Jining City	胶东丘陵区 Hill regions of eastern Shandong	QD-HTD	青岛市莱西市河头店镇 Hetoudian Town, Laixi City, Qingdao City
	JNI-HG	济宁市梁山县韩岗镇 Hangang Town, Liangshan County, Jining City		QD-YS	青岛市平度市云山镇 Yunshan Town, Pingdu City, Qingdao City
	JNI-LT	济宁市鱼台县罗屯镇 Luotun Town, Yutai County, Jining City		RZ-KG	日照市五莲县叩关镇 Kouguan Town, Wulian County, Rizhao City
	JNI-NZ	济宁市任城区南张镇 Nanzhang Town, Rencheng District, Jining City		RZ-XD	日照市莒县小店镇 Xiaodian Town, Ju County, Rizhao City
	JNI-TL	济宁市嘉祥县瞳里镇 Tongli Town, Jiaxiang County, Jining City		YT-SH	烟台市莱州市沙河镇 Shahe Town, Laizhou City, Yantai City
	JNI-WS	济宁市汶上县汶上镇 Wenshang Town, Wenshang County, Jining City	胶潍河谷平原区 Plain regions of middle Shandong	WF-BFZ	潍坊市安丘市白芬子镇 Baifenzi Town, Anqiu City, Weifang City
	JNI-WZ	济宁市曲阜市王庄镇 Wangzhuang Town, Qufu City, Jining City		WF-CG	潍坊市高密市柴沟镇 Chaigou Town, Gaomi City, Weifang City
	JNI-XX	济宁市曲阜市小雪镇 Xiaoxue Town, Qufu City, Jining City		WF-YQ	潍坊市昌乐县营丘镇 Yingqiu Town, Changle County, Weifang City
	LW-K	莱芜市莱城区口镇 Kou Town, Laicheng District, Laiwu City		WF-YS	潍坊市临朐县沂山镇 Yishan Town, Linqu County, Weifang City
	JNA-GD	济南市长清区归德镇 Guide Town, Changqing District, Ji’nan City		WF-ZG	潍坊市诸城市枳沟镇 Zhigou Town, Zhucheng City, Weifang City

表1

表2

图1

表3

节节麦种群对甲基二磺隆抗性水平"

区域 Region	种群编号 Code name	回归方程 Regression equation (y=)	相关系数 Correlation coefficient	GR₅₀ (95% CL)	相对抗性指数 RI
西北平原区 The northwest plain region	DZ-BMC	1.6414+2.3823x	0.9571	25.69 (19.64-37.22)	5.75
	DZ-DJ	2.7510+1.7126x	0.9625	20.57 (14.84-30.88)	4.60
	DZ-EC	0.8933+2.3445x	0.9540	56.45 (43.99-79.63)	12.63
	DZ-FS	2.5721+1.9455x	0.9801	17.00 (15.38-20.55)	3.80
	DZ-GJ	3.1395+1.3536x	0.979	23.69 (19.48-29.72)	5.30
	DZ-HJ	2.9286+1.3574x	0.9473	33.57 (21.89-67.27)	7.51
	DZ-JMY	1.8215+2.0327x	0.9382	36.61 (30.40-46.21)	8.19
	DZ-M	2.9115+1.7662x	0.9258	15.22 (9.26-25.92)	3.40
	DZ-WC	3.2068+1.3602x	0.9760	20.81 (17.23-25.72)	4.66
	DZ-XSD	3.4077+0.9164x	0.9777	54.64 (37.97-92.98)	12.22
	LC-DYZ	2.4271+1.9942x	0.9035	19.51 (14.87-26.93)	4.36
	LC-HY	2.5370+1.8409x	0.9956	21.77 (18.69-25.83)	4.87
	LC-SD	3.2928+1.5040x	0.9649	13.65 (11.48-16.20)	3.05
	LC-XH	1.2459+2.7259x	0.9480	23.83 (17.05-40.16)	5.33
	LC-YQH	2.3542+1.8971x	0.9761	24.81 (21.22-29.72)	5.55
	LC-ZZZ	3.2431+1.1057x	0.9897	38.81 (29.51-55.77)	8.68
西南平洼区 The southwest plain region	HZ-DK	2.1165+1.6795x	0.9280	52.11 (28.73-219.78)	11.66
	HZ-HX	2.8763+1.7662x	0.9791	15.94 (13.71-18.61)	3.57
	HZ-LH	2.5985+1.7662x	0.9589	22.89 (15.62-39.02)	5.12
	HZ-SG	2.7730+1.7662x	0.9669	18.23 (13.51-25.76)	4.08
	HZ-TZ	2.4712+1.7662x	0.9907	27.03 (22.80-33.00)	6.05
区域 Region	种群编号 Code name	回归方程 Regression equation (y=)	相关系数 Correlation coefficient	GR₅₀ (95% CL)	相对抗性指数 RI
	JNI-GC	3.9007+1.0418x	0.9845	11.35 (8.82-14.34)	2.54
	JNI-HG	3.0402+1.4605x	0.9386	21.97 (14.75-37.52)	4.91
	JNI-LT	2.2569+1.7662x	0.9656	35.73 (24.77-63.73）	7.99
	JNI-NZ	2.4873+1.4998x	0.9814	47.35 (36.84-65.89)	10.59
	JNI-TL	2.4846+1.7662x	0.9233	26.56 (16.40-60.99)	5.94
	JNI-WS	3.7081+0.9489x	0.9851	22.98 (17.72-31.38)	5.14
	JNI-WZ	1.3495+2.5287x	0.9790	27.77 (24.09-32.90)	6.21
	JNI-XX	2.3354+2.1662x	0.9060	16.99 (12.98-22.90)	3.80
中部山区 The middle mountain region	LW-K	1.2173+3.1559x	0.9786	15.80 (14.24-17.60)	3.53
	JNA-GD	2.4627+2.0322x	0.9595	17.72 (12.96-25.45)	3.96
	JNA-LS	3.4260+1.3826x	0.9692	13.75 (11.42-16.53)	3.08
	JNA-TP	3.2598+1.2409x	0.9888	25.26 (20.41-32.53)	5.65
	JNA-XZ	2.6086+1.4977x	0.9578	39.51 (31.49-52.79)	8.84
	TA-CH	2.3271+2.0471x	0.9761	20.22 (15.20-28.67)	4.52
	TA-DY	3.3863+1.5408x	0.9507	11.15 (7.75-15.34)	2.49
	TA-HJL	3.1961+1.7662x	0.9598	10.50 (7.37-14.19)	2.35
	TA-MZ	3.6318+1.3342x	0.9862	10.61 (8.63-12.82)	2.37
	TA-SZ	3.9080+1.2523x	0.9711	4.47 (2.74-7.24)	1.00
	ZB-QL	2.1253+2.0366x	0.9093	25.79 (19.14-38.90)	5.77
北部滨海区 The northern coastal region	ZB-QC	3.5432+1.4877x	0.9274	9.53 (5.03-15.15)	2.13
	BZ-TZ	2.2802+2.1230x	0.9649	19.10 (14.09-27.63)	4.27
	BZ-HJ	3.9106+1.1976x	0.9623	8.12 (6.28-10.10)	1.82
	BZ-LD	3.7325+1.3620x	0.9341	8.52 (6.06-11.22)	1.73
	DY-BS	3.9200+1.2143x	0.9197	7.75 (5.33-10.47)	2.73
南部山区 The southern mountain region	LY-GQ	4.1081+1.1602x	0.9182	5.8718 (3.77-8.28)	3.32
	LY-LZ	3.1540+1.5768x	0.9530	14.82 (11.63-18.28)	1.98
	LY-QT	2.0506+2.5039x	0.9814	15.06 (13.38-17.00）	3.37
	LY-SL	3.5482+1.3820x	0.9893	11.23 (9.23-13.49)	2.51
	LY-TS	3.6364+1.3993x	0.9452	9.43 (6.85-12.24)	1.58
	LY-TY	3.9370+1.1220x	0.9868	8.86 (6.14-11.94)	2.11
	ZZ-ZW	3.4027+1.5723x	0.9868	10.37 (8.65-12.25)	2.32
胶东丘陵区 Hill regions of eastern Shandong	QD-HTD	3.9361+1.1657x	0.9143	8.18 (5.64-11.05)	1.83
	QD-YS	3.6976+1.2110x	0.9115	11.92 (8.78-15.44)	2.67
	RZ-KG	3.4653+1.6632x	0.9581	8.37 (6.06-10.84)	1.31
	RZ-XD	3.7148+1.2709x	0.9243	10.26 (7.46-13.37)	2.30
	YT-SH	3.6279+1.3024x	0.9201	11.31 (5.27-18.94)	2.53
胶潍河谷平原区 Plain regions of middle Shandong	WF-BFZ	3.5350+1.5615x	0.9498	8.67 (6.29-11.24)	1.86
	WF-CG	4.0989+1.1249x	0.9105	6.32 (4.11-8.86)	1.91
	WF-YQ	3.6888+1.4592x	0.9467	7.92 (5.60-10.44)	1.94
	WF-YS	3.6458+1.5950x	0.9586	7.06 (4.91-9.38)	1.77
	WF-ZG	3.6313+1.4882x	0.9472	8.31 (5.95-10.88)	1.41

表3

图2

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引物名称 Primer name	序列 Sequence	扩增位点 Amplification site
Jals-11	5′-CTGCCTCACAGAAATCTCC-3′	122, 197, 205, 376, 377
Jals-12	5′-TCCCCTTTTGTCAGCTCAT-3′	122, 197, 205, 376, 377
Jals-21	5′-AGATTGGCAAGAACAAGCAG-3′	574, 653, 654
Jals-22	5′-CTTATGACAGCACATCCCTACA-3′	574, 653, 654

节节麦在山东省冬小麦田的扩散蔓延及对甲基二磺隆抗性测定

Spread and Resistance Level of Aegilops tauschii to Mesosulfuron- Methyl in Winter Wheat Field of Shandong Province

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