关键词: 花生, 油酸脱氢酶基因, 遗传转化, 实时荧光定量PCR

Abstract:

【Objective】 This article aims at breeding of new materials with high oleate in peanut. (Method) Plant dual expression vector pFGC/2nd, containing oleic desaturase gene, was transferred into full bud explants of peanut via Agrobacterium tumefaciens, and trsnsgenic plants were got by screening with kanamycin. 【Result】 It was better-performing that the bud explants were dipped into Agrobacterium suspension (OD600=0.6) for 8 - 10 min and then cultured on MS medium in the dark for three days. After induction screening for three months or so, a total of 16 conversion plants were produced, of which 11 transgenic plants were positive by PCR analysis. It stated that the foreign gene had been integrated into the receptor plant. The further Real Time PCR indicated that the expression level of four transgenic plants was significantly reduced. 【Conclusion】 It is speculated that target genes maybe suppressed by dsRNA interference, and the high oleate material could be selected.

Key words: peanut, oleate desaturase, genetic transformation, real-time fluorescent quantitative PCR