关键词: 热休克蛋白, 荧光定量PCR, 原位杂交, 组织损伤, 运输应激, 猪

Abstract: Abstract: 【OBJECTIVE】To demonstrate the relationship between the distribution, transcription level of HSPs mRNA and immunity tissue pathological lesion of transport stressed pigs. 【METHOD】In situ hybridization, one-step fluorescence quantitative and histopathological methods were used to invest pathological lesion, HSPs mRNA distribution and transcription level in lymph node and spleen of transport stressed pigs. 【RESULTS】Obvious acute histopathological changes were observed in lymph node and spleen at the beginning of transport stress (1-2 h). The level of HSP70 mRNA transcription increased continuously from the beginning of transportation. The inductions of HSP70 mRNA transcription in lymph node and spleen of 10 h transport stressed pigs were 9.59 and 11.46 times higher than that of the un-transport stressed pigs(P<0.01). However, the transcription levels of HSP90 mRNA increased only at the beginning of transport stress, 59.67 and 13.03 times higher than that of the un-transport stressed pigs at 2 h transport stressed pigs in lymph node and spleen, respectively. The location of HSP70 and HSP90 mRNA don’t change with the transport stress. 【CONCLUSION】Transport stress can affect the transcription of HSPs mRNA, and the effect is different between HSPs families. Furthermore, there are good relationship between the concentration of HSP90 mRNA and pathological lesion of lymph node and spleen, and HSP90 mRNA might be a hallmark to determine stress.

Key words: Heat shock protein, FQ-PCR, in situ hybridization, pathological lesion, Transport stress