关键词: 柿, 单宁, 蛇毒, 酶, 抑制作用, 机理

Abstract: 【Objective】To study the inhibitory effects of persimmon tannins (PT) on the activities of proteolytic enzyme, phospholipase A2, L-amino acid oxidase, arginine esterase, acetylcholinesterase and thrombin-like enzyme from Agkistrodon halys Pallas, Agkistrodon acutus, Naja naja atra venom , and the action mechanism between venom and PT. 【Method】 Different amounts of PT were mixed with snake venoms, after incubation at 37℃ for 1h and centrifuge, the enzyme activities of proteolytic enzyme, phospholipase A2, L-amino acid oxidase, arginine esterase, acetylcholinesterase and thrombin-like enzyme from the supernatants were mensurated, and the precipitates , supernatants and venom were analyzed by SDS-PAGE.【Result】The enzyme activities of proteolytic enzyme, phospholipase A2, L-amino acid oxidase, arginine esterase, acetylcholinesterase, and thrombin like enzyme were inhibited completely when the rate of snake venom and PT reached 1:1 (w/w), and the inhibitory effect was in a dose-dependent manner; PT was found to have very high combinative ability with venom proteins in a non-specifically manner.，but with different selectivity for different protein.【Conclusion】It was suggested that the enzyme activities from snake venoms could be inhibited remarkably by persimmon tannin in vitro by non-specifically combination.

Key words: persimmon tannin, snake venom, inhibition, SDS-PAGE