Characterization and expression analysis of a novel RING-HC gene, ZmRHCP1, involved in brace root development and abiotic stress responses in maize
LI Wen-lan, SUN Qi, LI Wen-cai, YU Yan-li, ZHAO Meng, MENG Zhao-dong
Maize Research Institute, Shandong Academy of Agricultural Sciences/National Engineering Laboratory of Wheat and Maize/Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-Huai River Plain, Ministry of Agriculture, Jinan 250100, P.R.China
AbstractRING is a really interesting new gene which plays important regulatory roles in many developmental processes as well as in plant-environment interactions. In the present report, the ZmRHCP1 gene encoding a putative RING-HC protein was isolated from maize and characterized. The ZmRHCP1 protein contained 310 amino acid residues with a conserved RING-HC zinc-finger motif and two transmembrane (TM) domains. ZmRHCP1 was expressed ubiquitously in various organs (root, stem, leaf, seedling, immature ear, and tassel), but its transcript levels were higher in vegetative organs than in reproductive organs. Moreover, the expression pattern of ZmRHCP1 in brace roots indicated that ZmRHCP1 functions in brace root initiation. In addition, ZmRHCP1 expression was regulated by abiotic stresses. The expression results suggested that ZmRHCP1 plays important roles in brace root development and abiotic stress responses. The findings of the present study provide important information to help us understand the function of ZmRHCP1 in maize.
About author: LI Wen-lan, E-mail: firstname.lastname@example.org
Cite this article:
LI Wen-lan, SUN Qi, LI Wen-cai, YU Yan-li, ZHAO Meng, MENG Zhao-dong. Characterization and expression analysis of a novel RING-HC gene, ZmRHCP1, involved in brace root development and abiotic stress responses in maize[J]. Journal of Integrative Agriculture,
2017, 16(09): 1892-1899.
Baldacci-Cresp F, Moussawi J, Leple J C, Acker R V, Kohler A, Candiracci J, Twyffels L, Spokevicius A V, Bossinger G, Laurans F, Brunel N, Vermeersch M, Boerjan W, Jaziri M E, Baucher M. 2015. PtaRHE1, a Populus tremula×Populus alba RING-H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development. The Plant Journal, 82, 978-990.
Cheung M Y, Zeng N Y, Tong S W, Li F W Y, Zhao K J, Zhang Q, Sun S S M, Lam H M. 2007. Expression of a RING-HC protein from rice improves resistance to Pseudomonas syringae pv. tomato DC3000 in transgenic Arabidopsis thaliana. Journal of Experimental Botany, 58, 4147-4151.
Disch S, Anastasiou E, Sharma V K, Laux T, Fletcher J C, Lenhard M. 2006. The E3 ubiquitin ligase BIG BROTHER controls Arabidopsis organ size in a dosage-dependent manner. Current Biology, 16, 272-279.
Du Q L, Cui W Z, Zhang C H, Yu D Y. 2010. GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in soybean (Glycine max). Molecular Biology Reports, 37, 685-693.
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi T M, Maere S, Beemster G T S, Neyt P, Anami S, Robles P, Micol J L, Inze D, Lijsebettens M V. 2007. The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. The Plant Cell, 19, 417-432.
Freemont P S. 1993. The RING finger - A novel protein sequence motif related to the zinc finger. Annals of the New York Academy of Sciences, 684, 174-192.
Freemont P S, Hanson I M, Trowsdale J. 1991. A novel cysteine-rich sequence motif. Cell, 64, 483-484.
Hochholdinger F, Park W J, Sauer M, Woll K. 2004a. From weeds to crops: Genetic analysis of root development in cereals. Trends in Plant Science, 9, 42-48.
Hochholdinger F, Woll K, Sauer M, Dembinsky D. 2004b. Genetic dissection of root formation in maize (Zea mays) reveals root-type specific developmental programmes. Annals of Botany, 93, 359-368.
Jensen R B, Jensen K L, Jespersen H M, Skriver K. 1998. Widespread occurrence of a highly conserved RING-H2 zinc finger motif in the model plant Arabidopsis thaliana. FEBS Letters, 436, 283-287.
Kam J, Gresshoff P, Shorter R, Xue G P. 2007. Expression analysis of RING zinc finger genes from Triticum aestivum and identification of TaRZF70 that contains four RING-H2 domains and differentially responds to water deficit between leaf and root. Plant Science, 173, 650-659.
Ko J H, Yang S H, Han K H. 2006. Upregulation of an Arabidopsis RING-H2 gene, XERICO, confers drought tolerance through increased abscisic acid biosynthesis. The Plant Journal, 47, 343-355.
Koiwai H, Tagiri A, Katoh S, Katoh E, Ichikawa H, Minami E, Nishizawa Y. 2007. RING-H2 type ubiquitin ligase EL5 is involved in root development through the maintenance of cell viability in rice. The Plant Journal, 51, 92-104.
Laity J H, Lee B M, Wright P E. 2001. Zinc finger proteins: New insights into structural and functional diversity. Current Opinion in Structural Biology, 11, 39-46.
Li W L, Zhao X Y, Zhang X S. 2015. Genome-wide analysis and expression patterns of the YUCCA genes in maize. Journal of Genetics and Genomics, 42, 707-710.
Li Y J, Fu Y R, Huang J G, Wu C A, Zheng C C. 2011. Transcript profiling during the early development of the maize brace root via Solexa sequencing. FEBS Journal, 278, 156-166.
Li Y Z, Wu B J, Yu Y L, Yang G D, Wu C A, Zheng C C. 2011. Genome-wide analysis of the RING finger gene family in apple. Molecular Genetics and Genomics, 286, 81-94.
Lim S D, Yim W C, Moon J C, Kim D S, Lee B M, Jang C S. 2010. A gene family encoding RING finger proteins in rice: Their expansion, expression diversity, and co-expressed genes. Plant Molecular Biology, 72, 369-380.
Liu L F, Jin L, Huang X H, Geng Y T, Li F, Qin Q Q, Wang R, Ji S Y, Zhao S S, Xie Q, Wei C H, Xie C, Ding B, Li Y. 2014. OsRFPH2-10, a ring-H2 finger E3 ubiquitin ligase, is involved in rice antiviral defense in the early stages of rice dwarf virus infection. Molecular Plant, 7, 1057-1060.
Nodzon L A, Xu W H, Wang Y, Pi L Y, Chakrabarty P K, Song W Y. 2004. The ubiquitin ligase XBAT32 regulates lateral root development in Arabidopsis. The Plant Journal, 40, 996-1006.
Park G G, Park J J, Yoon J M, Yu S N, An G H. 2010. A RING finger E3 ligase gene, Oryza sativa delayed seed germination 1 (OsDSG1), controls seed germination and stress responses in rice. Plant Molecular Biology, 74, 467-478.
Sahin-Cevik M, Moore G A. 2006. Isolation and characterization of a novel RING-H2 finger gene induced in response to cold and drought in the interfertile Citrus relative Poncirus trifoliata. Physiologia Plantarum, 126, 153-161.
Saurin A J, Borden K L B, Boddy M N, Freemont P S. 1996. Does this have a familiar RING? Trends in Biochemical Sciences, 21, 208-214.
Schwechheimer C, Willige B C, Zourelidou M, Dohmann E M N. 2009. Examining protein stability and its relevance for plant growth and development. Methods in Molecular Biology, 479, 147-171.
Serrano M, Guzman P. 2004. Isolation and gene expression analysis of Arabidopsis thaliana mutants with constitutive expression of ATL2, an early elicitor-response RING-H2 zinc-finger gene. Genetics, 167, 919-929.
Stone S L, Hauksdottir H, Troy A, Herschleb J, Kraft E, Callis J. 2005. Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis. Plant Physiology, 137, 13-30.
Tian M M, Lou L J, Liu L J, Yu F F, Zhao Q Z, Zhang H W, Wu Y R, Tang S Y, Xia R, Zhu B G, Serino G, Xie Q. 2015. The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt-stress response in Arabidopsis thaliana. The Plant Journal, 82, 81-92.
Varney G T C M. 1993. Rates of water uptake into the mature root system of maize plants. New Phytologist, 123, 775-786.
Wang X L M M, Canny M J. 1994. The branch roots of Zea. IV. The maturation and openness of xylem conduits in first-order branches of soil-grown roots. New Phytologist, 126, 21-29.
Xu R, Li Q Q. 2003. A RING-H2 zinc-finger protein gene RIE1 is essential for seed development in Arabidopsis. Plant Molecular Biology, 53, 37-50.
Zhao X Y, Cheng Z J, Zhang X S. 2006. Overexpression of TaMADS1, a SEPALLATA-like gene in wheat, causes early flowering and the abnormal development of floral organs in Arabidopsis. Planta, 223, 698-707.