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| Ammonium-dependent regulation of ammonium transporter ZmAMT1s expression conferred by glutamine levels in roots of maize |
| HUI Jing*, LIU Zhi*, DUAN Feng-ying, ZHAO Yang, LI Xue-lian, AN Xia, WU Xiang-yu, YUAN Li-xing |
| College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, P.R.China |
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摘要
本文利用玉米水培分根体系发现，局部供铵到缺氮根系可以显著诱导ZmAMT1;1a与ZmAMT1;3基因的表达水平。测定15N标记铵吸收速率与根组织氨基酸含量发现，铵诱导的ZmAMT1s基因表达与根系铵吸收能力及谷氨酰胺含量显著正相关。外界添加谷氨酰胺合成酶抑制剂MSX时，供铵则不能诱导缺氮根系中ZmAMT1;1a与ZmAMT1;3基因的表达，表明铵的同化产物谷氨酰胺，而不是铵本身，负责调控ZmAMT1s基因表达。此外，外界供应不同浓度谷氨酰胺到缺氮根系发现，较低浓度的谷氨酰胺就能够诱导ZmAMT1s基因表达，但高浓度谷氨酰胺却能够抑制其表达。以上研究结果表明，为了严格调节玉米根系铵吸收能力，铵转运蛋白ZmAMT1s基因在转录水平被严谨调控，与根内谷氨酰胺水平密切相关。
Abstract
In maize, two root epidermis-expressed ammonium transporters ZmAMT1;1a and ZmAMT1;3 play major roles in high-affinity ammonium uptake. However, the transcriptional regulation of ZmAMT1s in roots for ensuring optimal ammonium acquisition remains largely unknown. Here, using a split root system we showed that ZmAMT1;1a and ZmAMT1;3 transcript levels were induced by localized ammonium supply to nitrogen-deficient roots. This enhanced expression of ZmAMT1s correlated with increases in 15NH4+ influx rates and tissue glutamine concentrations in roots. When ammonium was supplied together with methionine sulfoximine, an inhibitor of glutamine synthase, ammonium-induced expression of ZmAMT1s disappeared, suggesting that glutamine rather than ammonium itself regulated ZmAMT1s expression. When glutamine was supplied to nitrogen-deficient roots, expression levels of ZmAMT1s were enhanced, and negative feedback regulation could subsequently occur by supply of glutamine at a high level. Thus, our results indicated an ammonium-dependent regulation of ZmAMT1s at transcript levels, and a dual role of glutamine was suggested in the regulation of ammonium uptake in maize roots.
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Received: 18 March 2021
Accepted: 19 May 2021
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Fund: This work was financially supported by the National Natural Science Foundation of China (31471934 and 30971863) and the Major Project of China on New Varieties of GMO Cultivation (2016ZX08003-005).
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| About author: HUI Jing, E-mail: 1244508876@qq.com; Correspondence YUAN Li-xing, Tel: +86-10-62734424, E-mail: yuanlixing@cau.edu.cn
* These authors contributed equally to this study. |
Cite this article:
HUI Jing, LIU Zhi, DUAN Feng-ying, ZHAO Yang, LI Xue-lian, AN Xia, WU Xiang-yu, YUAN Li-xing.
2022.
Ammonium-dependent regulation of ammonium transporter ZmAMT1s expression conferred by glutamine levels in roots of maize. Journal of Integrative Agriculture, 21(8): 2413-2421.
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