水稻早衰突变体es5的鉴定及其突变基因的精细定位

doi:10.3864/j.issn.0578-1752.2018.04.002

Abstract

Abstract: 【Objective】In this study genetic analysis and gene mapping of es5, a mutant with early senescent leaf in rice, were conducted to explore the molecular mechanism of rice leaf senescence.【Method】 es5 was isolated from the progeny of japonica rice cv. Jiahe212 treated by ethyl methane sulfonate (EMS). F2 population was derived from the cross between es5 and Zhonghui8015. The phenotypes of the F1 and the segregation ratio of the F2 were investigated in Hangzhou and Hainan separately. The recessive individuals in F2 were used to locate the gene by the map cloning method. The SOD activity, MDA content, soluble protein content, chlorophyll content, the net photosynthetic rate, the histochemical analysis and the expression of senescence-related genes were analyzed for es5 and Jiahe212 leaves at heading stage. Several main agronomic traits of es5 and Jiahe212 were analyzed under field conditions at maturation stage in Hangzhou.【Result】There was no difference between Jiahe212 and es5 before 4-leaf-stage. Leaf senescence in es5 occurred at 4-leaf-stage. The etiolated area of the rice leaf increased gradually with the growth and development. At elongation stage, the middle and lower leaves showed serious aging. Compared with the Jiahe212, Chl a, Chl b, carotenoid content and photosynthetic capacity of es5 significantly decreased and the plant height, the number of productive panicles per plant, the number of spikelets per panicle, seed setting rate and 1 000-grain weight also significantly reduced. The results of Evans blue and DAB staining assay indicated that there were more dead cells and H2O2 in leaves of es5. At 7 days to 21 days and heading, the SOD activities increased significantly in es5, MDA content increased significantly in 21days after heading, while the soluble protein content significantly decreased at 7 days and 21 days after heading. Dissolved cytoplasm, abnormal chloroplast structure, dissolved chloroplast membrane, blurred base, loose lamellar lamellae, abnormal thylakoid development and more starches grains and osmiophilic granules were observed in the senescent leaf region of es5 by Transmission Electronic Microscope (TEM). qRT-PCR analysis showed that senescence-associated genes Osh36, Osh69, OsI85, RCCR1 were up regulated significantly in es5. Especially, the expression of Osh69 and OsI85, two marker genes about aging, were up regulated 12.7 and 36.6 times respectively. SGR, a gene about degradation of chlorophyll, was up regulated significantly at the same time. Genetic analysis suggested that the phenotype of es5 was controlled by single recessive nuclear gene, and ES5 was fine mapped in a 52.7 kb interval including 8 open reading frames between BF-10 and RM3664 on chromosome 5 by map-based cloning strategy.【Conclusion】In this study several agricultural traits significantly in es5 for the leaf senescence ES5 was located in a 52.7 kb range between BF-10 and RM3664 by map-based cloning strategy.

Key words: Oryza sativa L., early senescence, es5, map-based cloning, fine mapping

WANG BeiFang, CHEN YuYu, ZHANG YingXin, LIU QunEn, SUN Bin, XIANG XiaoJiao, CAO YongRun, CHENG ShiHua, CAO LiYong. Identification and Fine Mapping of an Early Senescent Leaf Mutant es5 in Oryza sativa L.[J].Scientia Agricultura Sinica, 2018, 51(4): 613-625.

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