|
|
|
|
|
| Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils |
| WANG Xiu-rong, SU Shi-ming, ZENG Xi-bai, BAI Ling-yu, LI Lian-fang, DUAN Ran, WANG Ya-nan, WU Cui-xia |
| Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing 100081, P.R.China |
|
|
|
摘要 Fungi capable of arsenic (As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in field soils. In this study, the chlamydospores of Trichoderma asperellum SM-12F1 capable of As resistance, accumulation, and volatilization were inoculated into As-contaminated Chenzhou (CZ) and Shimen (SM) soils, and subsequently As volatilization and availability were assessed. The results indicated that T. asperellum SM-12F1 could reproduce well in As-contaminated soils. After cultivated for 42 days, the colony forming units (cfu) of T. asperellum SM-12F1 in CZ and SM soils reached 1010–1011 cfu g–1 fresh soil when inoculated at a rate of 5.0%. Inoculation with chlamydospores of T. asperellum SM-12F1 could significantly accelerate As volatilization from soils. The contents of volatilized As from CZ and SM soils after being inoculated with chlamydospores at a rate of 5.0% for 42 days were 2.0 and 0.6 μg kg–1, respectively, which were about 27.5 and 2.5 times higher than their corresponding controls of no inoculation (CZ, 0.1 μg kg–1; SM, 0.3 μg kg–1). Furthermore, the available As content in SM soils was decreased by 23.7%, and that in CZ soils increased by 3.3% compared with their corresponding controls. Further studies showed that soil pH values significantly decreased as a function of cultivation time or the inoculation level of chlamydospores. The pH values in CZ and SM soils after being inoculated with 5.0% of chlamydospores for 42 days were 6.04 and 6.02, respectively, which were lowered by 0.34 and 1.21 compared with their corresponding controls (CZ, 6.38; SM, 7.23). The changes in soil pH and As-binding fractions after inoculation might be responsible for the changes in As availability. These observations could shed light on the future remediation of As-contaminated soils using fungi.
Abstract Fungi capable of arsenic (As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in field soils. In this study, the chlamydospores of Trichoderma asperellum SM-12F1 capable of As resistance, accumulation, and volatilization were inoculated into As-contaminated Chenzhou (CZ) and Shimen (SM) soils, and subsequently As volatilization and availability were assessed. The results indicated that T. asperellum SM-12F1 could reproduce well in As-contaminated soils. After cultivated for 42 days, the colony forming units (cfu) of T. asperellum SM-12F1 in CZ and SM soils reached 1010–1011 cfu g–1 fresh soil when inoculated at a rate of 5.0%. Inoculation with chlamydospores of T. asperellum SM-12F1 could significantly accelerate As volatilization from soils. The contents of volatilized As from CZ and SM soils after being inoculated with chlamydospores at a rate of 5.0% for 42 days were 2.0 and 0.6 μg kg–1, respectively, which were about 27.5 and 2.5 times higher than their corresponding controls of no inoculation (CZ, 0.1 μg kg–1; SM, 0.3 μg kg–1). Furthermore, the available As content in SM soils was decreased by 23.7%, and that in CZ soils increased by 3.3% compared with their corresponding controls. Further studies showed that soil pH values significantly decreased as a function of cultivation time or the inoculation level of chlamydospores. The pH values in CZ and SM soils after being inoculated with 5.0% of chlamydospores for 42 days were 6.04 and 6.02, respectively, which were lowered by 0.34 and 1.21 compared with their corresponding controls (CZ, 6.38; SM, 7.23). The changes in soil pH and As-binding fractions after inoculation might be responsible for the changes in As availability. These observations could shed light on the future remediation of As-contaminated soils using fungi.
|
|
Received: 30 November 2013
Accepted:
|
| Fund: the financial support from the National Natural Science Foundation of China (41101296), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD14B02). |
|
Corresponding Authors:
ZENG Xi-bai,Tel: +86-10-82105612, E-mail: zengxibai@caas.cn
E-mail: zengxibai@caas.cn
|
| About author: WANG Xiu-rong, Tel: +86-10-82106009, E-mail: 124922870wxr@ sina.com; SU Shi-ming, Tel: +86-10-82106009, E-mail: sushiming@caas.cn; * These authors contributed equally to this study. |
Cite this article:
WANG Xiu-rong, SU Shi-ming, ZENG Xi-bai, BAI Ling-yu, LI Lian-fang, DUAN Ran, WANG Ya-nan, WU Cui-xia.
2015.
Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils. Journal of Integrative Agriculture, 14(2): 389-397.
|
| Achal V, Pan X L, Fu Q L, Zhang D Y. 2012. Biomineralizationbased remediation of As (III) contaminated soil bySporosarcina ginsengisoli. Journal of Hazardous Materials,201, 178-184?erňanský S, Kolen?ík M, Ševc J, Urík M, Hiller E 2009.Fungal volatilization of trivalent and pentavalent arsenicunder laboratory conditions. Bioresource Technology, 100,1037-1040Dixit S, Hering J G. 2003. Comparison of arsenic (V) andarsenic (III) sorption onto iron oxide minerals: Implicationsfor arsenic mobility. Environmental Science & Technology,37, 4182-4189Edvantoro B B, Naidu R, Megharaj M, Merrington G, SingletonI. 2004. Microbial formation of volatile arsenic in cattle dipsite soils contaminated with arsenic and DDT. Applied SoilEcology, 25, 207-217Elad Y, Chet I. 1983. Improved selective media for isolationof Trichoderma spp. or Fusarium spp. Phytoparasitica,11, 55-58Fayiga A O, Ma L Q. 2006. Using phosphate rock toimmobilize metals in soil and increase arsenic uptakeby hyperaccumulator Pteris vittata. Science of the TotalEnvironment, 359, 17-25Gadd G M. 2004. Microbial influence on metal mobility andapplication for bioremediation. Geoderma, 122, 109-119Huang H, Jia Y, Sun G X, Zhu Y G. 2012. Arsenic speciationand volatilization from flooded paddy soils amendedwith different organic matters. Environmental Science &Technology, 46, 2163-2168Huang J H, Hu K N, Decker B. 2011. Organic arsenic in thesoil environment: speciation, occurrence, transformation,and adsorption behavior. Water, Air, & Soil Pollution, 219,401-415Lewis J, Papavizas G. 1983. Production of chlamydosporesand conidia by Trichoderma spp. in liquid and solid growthmedia. Soil Biology and Biochemistry, 15, 351-357Liu S, Zhang F, Chen J, Sun G X. 2011. Arsenic removalfrom contaminated soil via biovolatilization by geneticallyengineered bacteria under laboratory conditions. Journalof Environmental Sciences, 23, 1544-1550Mestrot A, Feldmann J, Krupp E M, Hossain M S, Roman-RossG, Meharg A A. 2011. Field fluxes and speciation of arsinesemanating from soils. Environmental Science & Technology,45, 1798-1804Mestrot A, Uroic M K, Plantevin T, Islam M R, Krupp E M,Feldmann J R, Meharg A A. 2009. Quantitative and qualitative trapping of arsines deployed to assess loss ofvolatile arsenic from paddy soil. Environmental Science &Technology, 43, 8270-8275Papavizas G. 1982. Survival of Trichoderma harzianum in soiland in pea and bean rhizospheres. Phytopathology, 72,121-125Pokhrel D, Viraraghvavan T. 2008. Arsenic removal from anaqueous solution by modified A. niger biomass: Batchkinetic and isotherm studies. Journal of HazardousMaterials, 150, 818-825Qin J, Lehr C R, Yuan C, Le X C, McDermott T R, Rosen BP. 2009. Biotransformation of arsenic by a Yellowstonethermoacidophilic eukaryotic alga. Proceedings of theNational Academy of Sciences of the United States ofAmerica, 106, 5213-5217Sneh B, Dupler M, Elad Y, Baker R. 1984. Chlamydosporegermination of Fusarium oxysporum f. sp. cucumerinum asaffected by fluorescent and lytic bacteria from a Fusariumsuppressivesoil. Phytopathology, 74, 1115-1124Srivastava P K, Vaish A, Dwivedi S, Chakrabarty D, Singh N,Tripathi R D. 2011. Biological removal of arsenic pollutionby soil fungi. Science of the Total Environment, 409,2430-2442Su S M, Zeng X B, Bai L Y, Li L F, Duan R. 2011. Arsenicbiotransformation by arsenic-resistant fungi Trichodermaasperellum SM-12F1, Penicillium janthinellum SM-12F4,and Fusarium oxysporum CZ-8F1. Science of the TotalEnvironment, 409, 5057-5062Su S M, Zeng X B, Jiang X L, Li L F. 2010. Bioaccumulationand biovolatilisation of pentavalent arsenic by Penicillinjanthinellum, Fusarium oxysporum and Trichodermaasperellum under laboratory conditions. CurrentMicrobiology, 61, 261-266Su S M, Zeng X B, Li L F, Duan R, Bai L Y, Li A G, Wang J,Jiang S. 2012. Arsenate reduction and methylation in thecells of Trichoderma asperellum SM-12F1, Penicilliumjanthinellum SM-12F4, and Fusarium oxysporum CZ-8F1investigated with X-ray absorption near edge structure.Journal of Hazardous Materials, 243, 364-367Tabak H H, Lens P, van Hullebusch E D, Dejonghe W. 2005.Developments in bioremediation of soils and sedimentspolluted with metals and radionuclides -1 Microbialprocesses and mechanisms affecting bioremediation ofmetal contamination and influencing metal toxicity andtransport. Reviews in Environmental Science and Bio/Technology, 4, 115-156Wang P P, Sun G X, Jia Y, Meharg A A, Zhu Y G. 2013.A review on completing arsenic biogeochemical cycle:Microbial volatilization of arsines in environment. Journalof Environmental Sciences, 26, 371-381Wang S L, Zhao X Y. 2009. On the potential of biologicaltreatment for arsenic contaminated soils and groundwater.Journal of Environmental Management, 90, 2367-2376Wenzel W W, Kirchbaumer N, Prohaska T, Stingeder G, LombiE, Adriano D C. 2001. Arsenic fractionation in soils usingan improved sequential extraction procedure. AnalyticaChimica Acta, 436, 309-323Woolson E, Axley J, Kearney P. 1971. Correlation betweenavailable soil arsenic, estimated by six methods, andresponse of corn (Zea mays L.). Soil Science Society ofAmerica Journal, 35, 101-105Ye J, Rensing C, Rosen B P, Zhu Y G. 2012. Arsenicbiomethylation by photosynthetic organisms. Trends inPlant Science, 17, 155-162Zeng X B, Su S M, Jiang X L, Li L F, Bai L Y, Zhang Y R. 2010.Capability of pentavalent arsenic bioaccumulation andbiovolatilization of three fungal strains under laboratoryconditions. CLEAN-Soil, Air, Water, 38, 238-241Zhang J Y, Ding T D, Zhang C L. 2013. Biosorption andtoxicity responses to arsenite (As [III]) in Scenedesmusquadricauda. Chemosphere, 92, 1077-1084Zhao F J, Zhu Y G, Meharg A A. 2013. Methylated arsenicspecies in rice: Geographical variation, origin, and uptakemechanisms. Environmental Science & Technology, 47,3957-3966Zheng R L, Sun G X, Zhu Y G. 2013. Effects of microbialprocesses on the fate of arsenic in paddy soil. ChineseScience Bulletin, 58, 186-193 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
