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| SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum |
| SUN Xiao-chun, GAO Yong-feng, ZHANG Ning, LI Hui-rong, YANG Shu-zhang, LIU Yong-sheng |
1、Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment/State Key Laboratory of Hydraulics and Mountain
River Engineering/College of Life Science, Sichuan University, Chengdu 610064, P.R.China
2、School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, P.R.China |
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摘要 SOM encodes a nucleus-localized CCCH-type zinc finger protein and negatively regulates seed germination in Arabidopsis thaliana. We have previously demonstrated that ectopic expression of SlABI3, an important transcription factor in abscisic acid (ABA) signaling pathway, resulted in alteration of SlSOM expression patterns in both leaf and seed of tomato. In this study, we aimed to elucidate the function of tomato SlSOM in regarding to seed germination and seedling development. Here, we constructed SlSOM over-expression vector pBI121-SOM driven by CaMV 35S promoter, and the recombinant plasmid was incorporated into wild-type tomato by the method of Agrobacterium tumefaciens-mediated transformation. The result showed that over-expression of SlSOM conferred enhanced responses to exogenous ABA application during seed germination and seedling development. In addition, ectopic expression of SlSOM resulted in the alteration of expression level of ABA/GA (gibberellins) metabolic genes, such as SlABA1, SlCYP707A1, SlGA3ox2, and SlGA2ox4, in both leaf and seed. The ABA anabolic gene SlABA1 and the GA catabolic gene SlGA2ox4 were up-regulated while the ABA catabolic gene SlCYP707A1 and the GA anabolic gene SlGA3ox2 were down-regulated. Compared to wild type, the expression level of SlABI5 was increased by about 40–50% in transgenic seeds while adding exogenous ABA treatment. These results support the notion that SlSOM inhibits seed germination by regulating ABA/GA metabolic genes and SlABI5 expression in Solanum lycopersicum.
Abstract SOM encodes a nucleus-localized CCCH-type zinc finger protein and negatively regulates seed germination in Arabidopsis thaliana. We have previously demonstrated that ectopic expression of SlABI3, an important transcription factor in abscisic acid (ABA) signaling pathway, resulted in alteration of SlSOM expression patterns in both leaf and seed of tomato. In this study, we aimed to elucidate the function of tomato SlSOM in regarding to seed germination and seedling development. Here, we constructed SlSOM over-expression vector pBI121-SOM driven by CaMV 35S promoter, and the recombinant plasmid was incorporated into wild-type tomato by the method of Agrobacterium tumefaciens-mediated transformation. The result showed that over-expression of SlSOM conferred enhanced responses to exogenous ABA application during seed germination and seedling development. In addition, ectopic expression of SlSOM resulted in the alteration of expression level of ABA/GA (gibberellins) metabolic genes, such as SlABA1, SlCYP707A1, SlGA3ox2, and SlGA2ox4, in both leaf and seed. The ABA anabolic gene SlABA1 and the GA catabolic gene SlGA2ox4 were up-regulated while the ABA catabolic gene SlCYP707A1 and the GA anabolic gene SlGA3ox2 were down-regulated. Compared to wild type, the expression level of SlABI5 was increased by about 40–50% in transgenic seeds while adding exogenous ABA treatment. These results support the notion that SlSOM inhibits seed germination by regulating ABA/GA metabolic genes and SlABI5 expression in Solanum lycopersicum.
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Received: 31 March 2014
Accepted:
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| Fund: This work was supported by the National Science Fund for Distinguished Young Scholars, China (30825030), the National Natural Science Foundation of China (31171179, 90717110), the National Basic Research Program of China (973, 2011CB100401) and the Advanced Program of Doctoral Fund of Ministry of Education of China (20110181130009). |
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Corresponding Authors:
LIU Yong-sheng, Fax: +86-551-62919399;E-mail: liuyongsheng1122@hfut.edu.cn
E-mail: liuyongsheng1122@hfut.edu.cn
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| About author: S U N X i a o - c h u n , E - m a i l : s u n x i a o c h u n 0 8 @ 1 6 3 . c o m ; |
Cite this article:
SUN Xiao-chun, GAO Yong-feng, ZHANG Ning, LI Hui-rong, YANG Shu-zhang, LIU Yong-sheng.
2015.
SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum. Journal of Integrative Agriculture, 14(2): 326-336.
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| Agustí J, Zapater M, Iglesias D J, Cercós M, Tadeo FR, Talón M. 2007. Differential expression of putative9-cis-epoxycarotenoid dioxygenases and abscisic acidaccumulation in water stressed vegetative and reproductivetissues of citrus. Plant Science, 172, 85-94Bewley J D. 1997. Seed germination and dormancy. The PlantCell, 9, 1055-1066Bogamuwa S, Jang J C. 2013. The Arabidopsis tandem CCCHzinc finger proteins AtTZF4, 5 and 6 are involved in light-,abscisic acid- and gibberellic acid-mediated regulationof seed germination. Plant, Cell and Environment, 36,1507-1519Davies P J. 1995. Plant Hormones: Physiology, Biochemistryand Molecular Biology. Dordrecht, Kluwer Academic, TheNetherlands.DePauw R M, Knox R E, Singh A K, Fox S L, Humphreys D G, Hucl P. 2012. Developing standardized methods forbreeding preharvest sprouting resistant wheat, challengesand successes in Canadian wheat. Euphytica, 188, 7-14Fillatti J J, Kiser J, Rose R, Comai L. 1987. Efficient transferof a glyphosate tolerance gene into tomato using a binaryAgrobacterium tumefaciens vector. Nature Biotechnology,5, 726-730Finkelstein R R, Lynch T J. 2000. The Arabidopsis abscisicacid response gene ABI5 encodes a basic leucine zippertranscription factor. The Plant Cell, 12, 599-609Fischerova L, Fischer L, Vondrakova Z, Vagner M. 2008.Expression of the gene encoding transcription factor PaVP1differs in Picea abies embryogenic lines depending on theirability to develop somatic embryos. Plant Cell Reports, 27,435-441Gao Y F, Liu J K, Zhang Z G, Sun X C, Zhang N, Fan J, Niu XL, Xiao F M, Liu Y S. 2013. Functional characterization oftwo alternatively spliced transcripts of tomato ABSCISICACID INSENSITIVE3 (ABI3) gene. Plant Molecular Biology,82, 131-145Gutierrez L, Wuytswinkel O V, Castelain M, Bellini C. 2007.Combined networks regulating seed maturation. TRENDSin Plant Science, 12, 294-300Huang T, Qu B, Li H P, Zuo D Y, Zhao Z X, Liao Y C. 2012.A maize viviparous 1 gene increases seed dormancy andpreharvest sprouting tolerance in transgenic wheat. Journalof Cereal Science, 55, 166-173Huang Y L, Shen G L, Shi Y J, Wang W G, Zhang Z Z, Shi Y Y,Chen D P. 2008. Effect of different germinating conditionson rice qualities and RVA profile character in early Indicarice. Chinese Agricultural Science Bulletin, 24, 119-122(in Chinese)Kim D H, Yamaguchi S, Lim S, Oh E, Park J, Hanada A, KamiyaY, Choi G. 2008. SOMNUS, a CCCH-type zinc fingerprotein in arabidopsis, negatively regulates light-dependentseed germination downstream of PIL5. The Plant Cell, 20,1260-1277KushiroT, Okamoto M, Nakabayashi K, Yamagishi K, KitamuraS, Asami T, Hirai N, Koshiba T, Kamiya Y, Nambara E. 2004.The Arabidopsis cytochrome P450 CYP707A encodes ABA8´-hydroxylases: Key enzymes in ABA catabolism. TheEMBO Journal, 23, 1647-1656Leung J, Giraudat J. 1998. Abscisic acid signal transduction.Annual Review of Plant Physiology and Plant MolecularBiology, 49, 199-222Liu K, Jiang H, Moore S L, Watkins C B, Jahn M M. 2006.Isolation and characterization of a lipid transfer proteinexpressed in ripening fruit of Capsicum chinense. Planta,223, 672-683Lopez-Molina L, Mongrand S, Chua N H. 2001. A postgerminationdevelopmental arrest checkpoint is mediated by abscisic acidand requires the ABI5 transcription factor in Arabidopsis.Proceedings of the National Academy of Sciences of theUnited States of America, 98, 4782-4787Lopez-Molina L, Mongrand S, McLachlin D T, Chait B T, ChuaN H. 2002. ABI5 acts downstream of ABI3 to execute anABA-dependent growth arrest during germination. The PlantJournal, 32, 317-328McCarty D R, Hattori T, Carson C B, Vasil V, Lazar M, Vasil IK. 1991. The Viviparous-1 developmental gene of maizeencodes a novel transcriptional activator. Cell, 66, 895-905Nakashima K, Yamaguchi-Shinozaki K. 2013. ABA signaling instress-response and seed development. Plant Cell Reports,32, 959-970Nambara E, Naito S, McCourt P. 1992. A mutant of Arabidopsiswhich is defective in seed development and storage proteinaccumulation is a new abi3 allele. The Plant Journal, 2,435-441Nitsch L M C, Oplaat C, Feron R, Ma Q, Wolters-Arts M,Hedden P, Mariani C, Vriezen W H. 2009. Abscisic acidlevels in tomato ovaries are regulated by LeNCED1 andSlCYP707A1. Planta, 229, 1335-1346Ogawa M, Hanada A, Yamauchi Y, Kuwahara A, Kamiya Y,Yamaguchi S. 2003. Gibberellin biosynthesis and responseduring Arabidopsis seed germination. The Plant Cell, 15,1591-1604Oh E, Kang H, Yamaguchi S, Park J, Lee D, Kamiya Y, ChoiG. 2009. Genome-wide analysis of genes targeted byPHYTOCHROME INTERACTING FACTOR 3-LIKE5during seed germination in Arabidopsis. The Plant Cell,21, 403-419Oh E, Yamaguchi S, Kamiya Y, Bae G, Chung W, Choi G. 2006.Light activates the degradation of PIL5 protein to promoteseed germination through gibberellin in Arabidopsis. ThePlant Journal, 47, 124-139Okamoto M, Kuwahara A, Seo M, Kushiro T, Asami T, Hirai N,Kamiya Y, Koshiba T, Nambara E. 2006. CYP707A1 andCYP707A2, which encode abscisic acid 8´-hydroxylases,are indispensable for proper control of seed dormancy andgermination in Arabidopsis. Plant Physiology, 141, 97-107Park J, Lee N, Kim W, Lim S, Choi G. 2011. ABI3 and PIL5collaboratively activate the expression of SOMNUS bydirectly binding to its promoter in imbibed Arabidopsisseeds. The Plant Cell, 4, 1404-1415Piskurewicz U, Jikumaru Y, Kinoshita N, Nambara E, KamiyaY, Lopez-Molina L. 2008. The gibberellic acid signalingrepressor rgl2 inhibits arabidopsis seed germination bystimulating abscisic acid synthesis and ABI5 activity. ThePlant Cell, 20, 2729-2745Piskurewicz U, Turecˇkova´ V, Lacombeand E, Lopez-MolinaL. 2009. Far-red light inhibits germination through DELLAdependentstimulation of ABA synthesis and ABI3 activity.The EMBO Journal, 28, 2259-2271Reidt W, Wohlfarth T, Ellerstrom M, Czihal A, Tewes A, EzcurraI, Rask L, Baumlein H. 2000. Gene regulation during lateembryogenesis: The RY motif of maturation-specific genepromoters is a direct target of FUS3 gene product. ThePlant Journal, 21, 401-408Rock C D. 2000. Pathways to abscisic acid-regulated geneexpression. New Phytologist, 148, 357-396Rodríguez-Gacio M C, Matilla-Vázquez M A, Matilla A J. 2009.Seed dormancy and ABA signaling the breakthrough goes on. Plant Signaling & Behavior, 4, 1035-1048Sambrook J, Russell W R. 2001. Molecular Cloning: ALaboratory Manual. 3rd ed. Cold Spring Harbor LaboratoryPress, Cold Spring Harbor.Seo M, Hanada A, Kuwahara A, Endo A, Okamoto M, YamauchiY, North H, Marion-Poll A, Sun T P, Koshiba T, Kamiya Y,Yamaguchi S, Nambara E. 2006. Regulation of hormonemetabolism in Arabidopsis seeds: Phytochrome regulationof abscisic acid metabolism and abscisic acid regulation ofgibberellin metabolism. The Plant Journal, 48, 354-366Serrani J C, Sanjuán R, Ruiz-Rivero O, Fos M, García-MartínezJ L. 2007. Gibberellin regulation of fruit set and growth intomato. Plant Physiology, 145, 246-257Wang Y P, Li L, Ye T T, Zhao S J, Liu Z, Feng Y Q , Wu Y.2011. Cytokinin antagonizes ABA suppression to seedgermination of Arabidopsis by down regulating ABI5expression. The Plant Journal, 68, 249-261Xiao S H, Zhang X Y, Yan C S, Lin H. 2002. Germplasmimprovement for preharvest sprouting resistance in Chinesewhite-grained wheat: An overview of the current strategy.Euphytica, 126, 35-38Yamauchi Y, Takeda-Kamiya N, Hanada A, Ogawa M,Kuwahara A, Seo M, Kamiya Y, Yamaguchi S. 2007.Contribution of gibberellin deactivation by AtGA2ox2 to thesuppression of germination of dark-imbibed Arabidopsisthaliana seeds. Plant Cell Physiology, 48, 555-561Yao Y Y, Dong C H, Yi Y J, Li X, Zhang X M, Liu J Y. 2014.Regulatory function of AMP1 in ABA biosynthesis anddrought resistance in Arabidopsis. Journal of Plant Biology,57,117-126Zhang M, Leng P, Zhang G L, Li X X. 2009. Cloning andfunctional analysis of 9-cis-epoxycarotenoid dioxygenase(NCED) genes encoding a key enzyme during abscisic acidbiosynthesis from peach and grape fruits. Journal of PlantPhysiology, 166, 1241-1252Zhu J K, Xiong L. 2003. Regulation of abscisic acid biosynthesis.Plant Physiology, 133, 29-36 |
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