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SlGH9-15 regulates tomato fruit cracking with hormonal and abiotic stress responsiveness cis-elements
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| LIN Hao-wei, WU Zhen, ZHOU Rong, CHEN Bin, ZHONG Zhao-jiang, JIANG Fang-ling |
College of Horticulture, Nanjing Agricultural University/Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, Nanjing 210095, P.R.China
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摘要
在发育后期，如遇到不适宜的环境条件，果实极易发生开裂。裂果严重影响果实的生产和销售。本研究利用混池转录组测序（BSR- seq）技术发掘到番茄果实开裂的关键调控基因。研究通过BSR-Seq鉴定到与不规则裂果性状相关的两个区域，分别位于第9和第11号染色体，共包含127个候选基因。进一步通过差异表达分析以及在耐裂和易裂番茄中的qRT-PCR分析，筛选到显著差异表达的候选基因SlGH9-15 (Solyc09g010210)。对GH9基因家族进行生物信息学分析，发现20个SlGH9基因被分为3组。系统发育分析表明，SlGH9-15与细胞壁结构相关基因AtGH9B1, AtGH9B6, OsGH9B1和OsGH9B3密切相关。启动子顺式作用元件分析表明，SlGH9-15可被不同激素(乙烯和脱落酸)及非生物胁迫诱导表达。表达模式分析表明，13个SlGH9基因，特别是SlGH9-15在易裂果基因型中高表达，且随着果实发育成熟，表达量逐渐增加，在红熟期表达量达到最大值。此外，与耐裂果番茄相比，易裂果番茄中纤维素酶活性较高，纤维素含量较低，在红熟期两基因型中该基因表达差异更为显著。本研究首次鉴定到SlGH9-15基因，推测为番茄果实开裂的关键调控基因。研究为阐明番茄裂果的分子机制及其复杂的调控网络提供了新视野。
Abstract
Fruit cracking occurs easily during the late period of fruit development when plants encounter an unsuitable environment, dramatically affecting fruit production and marketing. This study conducted the bulked segregant RNA-Seq (BSR) to identify the key regulatory gene of fruit cracking in tomatoes. BSR-Seq analysis illustrated that two regions associated with irregularly cracking were located on chromosomes 9 and 11, containing 127 candidate genes. Further, through differentially expression analysis and qRT-PCR in cracking-susceptible and cracking-resistant genotypes, the candidate gene SlGH9-15 (Solyc09g010210) with significantly differential expression levels was screened. Bioinformatics analysis of the GH9 gene family revealed that 20 SlGH9 genes were divided into three groups. The phylogenetic analysis showed that SlGH9-15 was closely related to cell wall construction-associated genes AtGH9B1, AtGH9B6, OsGH9B1, and OsGH9B3. The cis-acting elements analysis revealed that SlGH9-15 was activated by various hormones (ethylene and ABA) and abiotic stresses. The expression pattern indicated that 13 SlGH9 genes, especially SlGH9-15, were highly expressed in the cracking-susceptible genotype. Its expression level gradually increased during fruit development and achieved maximum value at the red ripe stage. Additionally, the cracking-susceptible tomato showed higher cellulase activity and lower cellulose content than the cracking-resistant tomato, particularly at the red ripe stage. This study identified SlGH9-15 as a key gene associated with fruit cracking in tomatoes for the first time and gives new insights for understanding the molecular mechanism and complex regulatory network of fruit cracking
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Received: 01 April 2022
Accepted: 29 July 2022
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| Fund: This work was supported by the National Key Research and Development Program of China (2019YFD100190200), the Jiangsu Agricultural Science and Technology Innovation Fund, China (CX(20)3101), and a grant from the Fundamental Research Funds for the Central Universities, China (KYZZ2022004). |
| About author: LIN Hao-wei, E-mail: 2019104067@stu.njau.edu.cn; Correspondence JIANG Fang-ling, Tel: +86-25-84396251, Fax: +86-25-84399083, E-mail: jfl@njau.edu.cn |
Cite this article:
LIN Hao-wei, WU Zhen, ZHOU Rong, CHEN Bin, ZHONG Zhao-jiang, JIANG Fang-ling.
2023.
SlGH9-15 regulates tomato fruit cracking with hormonal and abiotic stress responsiveness cis-elements
. Journal of Integrative Agriculture, 22(2): 447-463.
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