|
|
|
|
|
| Proteomic Analysis of Fruit Bending in Cucumber (Cucumis sativus L.) |
| WANG Li-li, ZHANG Peng, QIN Zhi-wei , ZHOU Xiu-yan |
1、College of Horticulture, Northeast Agricultural University, Harbin 150030, P.R.China
2、Institute of Vegetable, Zhejiang Academy of Agriculture Sciences, Hangzhou 310021, P.R.China |
|
|
|
摘要 In cucumber, fruit shape is an important quality criterion, and fruit bending is known to limit growth, yield, and taste. To investigate the post-transcriptional changes that regulate fruit bending and to better understand the underlying molecular mechanisms, we generated a proteomic profile of the abdomen and back of cucumber bending fruit. Two-dimensional gel electrophoresis (2-DE) allowed the detection of approximately 900 distinct protein spots in each gel, 32 of which were differentially expressed in the abdomen and back of bending cucumber fruit. Ten of the differentially expressed proteins were analyzed using matrix-assisted laser ionization time of flight mass spectrometry (MALDI-TOF/MS). A search of primary databases showed that the identified proteins are involved in various metabolic processes and cellular responses, including photosynthesis metabolism, energy metabolism, defense and stress response, and regulation. The identified proteins included large subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase, which are involved in photosynthesis and photorespiratory metabolism, and isocitrate dehydrogenase, which is involved in the tricarboxylic acid cycle. It is possible that imbalances in catabolic and anabolic processes directly affect the bending of cucumber fruit. The predicted function of the cobalamin- independent methionine synthase isozyme is closely related to ethylene biosynthesis; fruit bending may be regulated by ethylene, or by ethylene signaling crosstalk during fruit development. The 14-3-3 protein is usually considered to be a regulation-related protein, which plays a role in regulating cell hyperplasia, cell differentiation during growth, and apoptosis during senescence. Involvement of guanosine triphosphate (GTP)-binding proteins in signal transmission is known to regulate the development of cells in cucumber fruits and to play a role in fruit shape variation. Patterns of protein expression showed high repeatability. We hypothesize that these proteins may play an important role in growth and bending of cucumber fruits. The results of our study provide insight into the genetic mechanism underlying fruit bending in cucumber, and may help to promote cultivation of new varieties with superior fruits.
Abstract In cucumber, fruit shape is an important quality criterion, and fruit bending is known to limit growth, yield, and taste. To investigate the post-transcriptional changes that regulate fruit bending and to better understand the underlying molecular mechanisms, we generated a proteomic profile of the abdomen and back of cucumber bending fruit. Two-dimensional gel electrophoresis (2-DE) allowed the detection of approximately 900 distinct protein spots in each gel, 32 of which were differentially expressed in the abdomen and back of bending cucumber fruit. Ten of the differentially expressed proteins were analyzed using matrix-assisted laser ionization time of flight mass spectrometry (MALDI-TOF/MS). A search of primary databases showed that the identified proteins are involved in various metabolic processes and cellular responses, including photosynthesis metabolism, energy metabolism, defense and stress response, and regulation. The identified proteins included large subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase, which are involved in photosynthesis and photorespiratory metabolism, and isocitrate dehydrogenase, which is involved in the tricarboxylic acid cycle. It is possible that imbalances in catabolic and anabolic processes directly affect the bending of cucumber fruit. The predicted function of the cobalamin- independent methionine synthase isozyme is closely related to ethylene biosynthesis; fruit bending may be regulated by ethylene, or by ethylene signaling crosstalk during fruit development. The 14-3-3 protein is usually considered to be a regulation-related protein, which plays a role in regulating cell hyperplasia, cell differentiation during growth, and apoptosis during senescence. Involvement of guanosine triphosphate (GTP)-binding proteins in signal transmission is known to regulate the development of cells in cucumber fruits and to play a role in fruit shape variation. Patterns of protein expression showed high repeatability. We hypothesize that these proteins may play an important role in growth and bending of cucumber fruits. The results of our study provide insight into the genetic mechanism underlying fruit bending in cucumber, and may help to promote cultivation of new varieties with superior fruits.
|
|
Received: 31 October 2012
Accepted:
|
| Fund: This study was conducted in Northeast Agriculture University, China and financially supported by the National High-Tech R&D Program of China (863 Program, 2006AA10Z1B9); the National Natural Science Foundation of China (NSFC, 31140058); the Program of Innovative Research Team for the Higher Education Institutions of Heilongjiang Province, China (2009td07). |
|
Corresponding Authors:
QIN Zhi-wei, Tel: +86-451-55190058, Fax: +86-451-55190058, E-mail: qzw303@126.com
E-mail: qzw303@126.com
|
| About author: WANG Li-li, Tel: +86-451-55191192, Fax: +86-451-55190058, E-mail: lily-0606@126.com; ZHANG Peng, Tel: +86-571-86411359, Fax: +86-571-86411359, E-mail:
yinxiang0586@126.com |
Cite this article:
WANG Li-li, ZHANG Peng, QIN Zhi-wei , ZHOU Xiu-yan.
2014.
Proteomic Analysis of Fruit Bending in Cucumber (Cucumis sativus L.). Journal of Integrative Agriculture, 13(5): 963-974.
|
| Ashida H, Danchin A, Yokota A. 2005. Was photosyntheticRuBisCo recruited by acquisitive evolution from RuBisCo-like proteins involved in sulfur metabolism.Research in Microbiology, 156, 611-618Assmann S M. 2002. Heterotrimeric and unconventional GTP binding proteins in plant cell signaling. The PlantCell, 14, s355-s373.Bai S Y, Willard B, Chapin L J, Kinter M T, Francis D M, Stead A D, Jones M L. 2010. Proteomic analysis of pollination-induced corolla senescence in petunia.Journal of Experimental Botany, 61, 1089-1109Berardini T Z, Mundodi S, Reiser L, Huala E, Garcia-Hernandez M, Zhang P, Mueller L A, Yoon J, Doyle A, Lander G, Moseyko N, Yoo D, Xu I, Zoeckler B,Montoya M, Miller N, Weems D, Rhee S Y. 2004.Functional annotation of the Arabidopsis genome usingcontrolled vocabularies. Plant Physiology, 135, 1-11Bischoff F, Molendijk A, Rajendrakumar C S, Palme K.1999. GTP-binding proteins in plants. Cellular andMolecular Life Sciences, 55, 233-56Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalyticalBiochemistry, 72, 248-254Cann K L, Hicks G G. 2007. Regulation of the cellular DNAdouble-strand break response. Biochemistry and Cell Biology (Biochimie et Biologie Cellulaire), 85, 663-674Chang W W, Huang L, Shen M, Webster C, Burlingame A L, Roberts J K. 2000. Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment, and identification of proteins by mass spectrometry. Plant Physiology, 122, 295-317 Chen S E, Hu J R, Wang Y. 2000. Japanese standards of cucumber and tomato introduction. China Vegetables, 3, 54-55 (in Chinese) Corpas F J, Juan B. Barroso J B, Sandalio L M, José M, Palma J M, José A. Lupiá?ez J A, Luis A. del Río L A. 1999. Peroxisomal NADP-dependent isocitrate dehydrogenase. characterization and activity regulation during natural senescence. Plant Physiology, 121, 921- 928. Dafny-Yelin M, Guterman I, Menda N, Ovadis M, Shalit M, Pichersky E, Zamir D, Lewinsohn E, Adam Z, Weiss D, Vainstein A. 2005. Flower proteome: Changes in protein spectrum during the advanced stages of rose petal development. Planta, 222, 37-46 Dalziel K. 1980. Isocitrate dehydrogenase and related oxidative decarboxylases. FEBS Letters, 117, 45-55 Desclos M, Etienne P, Coquet L, Jouenne T, Bonnefoy J, Segura R, Reze S, Ourry A, Avice J C. 2009. A combined 15N tracing/proteomics study in Brassica napus reveals the chronology of proteomics events associated with N remobilization during leaf senescence induced by nitrate limitation or starvation. Proteomics, 9, 3580-3608 Donnini S, Prinsi B, Negri A S, Vigani G, Espen L, Zocchi G. 2010. Proteomic characterization of iron efficiency responses in Cucumis sativus L. roots. BMC Plant Biology, 10, 268. van Doorn W G, Woltering E J. 2008. Physiology and molecular biology of petal senescence. Journal of Experimental Botany, 59, 453-480 Duncan R, Hershey J W. 1984. Evaluation of isoelectric focusing running conditions during two-dimensional i soelectric focusing/sodium dodecyl sulfate- polyacrylamide gel electrophoresis: Variation of gel patterns with changing conditions and optimized isoelectric focusing conditions. Analytical Biochemistry, 138, 144-155 Eichel J, González J C, Hotze M, Matthews R G, Schr?der J. 1995. Vitamin-B12-Independent methionine synthase from a higher plant (Catharanthus Roseus). European Journal of Biochemistry, 230, 1053-1058 Faurobert M, Mihr C, Bertin N, Pawlowski T, Negroni L,Sommerer N, Causse M. 2007. Major proteome variations associated with cherry tomato pericarp development and ripening. Plant Physiology, 143, 1327- 1346. Feller U, Anders I, Demirevska K, 2008. Degradation of Rubisco and other chloroplast proteins under abiotic stress. General and Applied Plant Physiology, Special Issue, 34, 5-18 Ferrer J L, Ravanel S, Robert M, Dumas R. 2004. Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate. Journal of Biological Chemistry, 279, 44235-44238 Fu H, Subramanian R R, Masters S C. 2000. 14-3-3 proteins: Structure, function, and regulation Annual Review of Pharmacology and Toxicology, 40, 617-647 G ao L, Gu X B, Yu D S, Yu R K, Zeng G. 1996. Association of a 14-3-3 protein with CMP-NeuAc: GM1 alpha 2,3-sialyltransferase Biochemical and Biophysical Research Communications, 224, 103-107. Ge C J, Qin Z W, Zhou X Y. 2009. Analysis on evaluation method for cucumber fruit bending and relativities. China Vegetables, 8, 28-31. (in Chinese) Ge C J. 2008. Evaluation of main germplasm resources about different degree of bending in cucumber. MSc thesis, Northeast Agricultural University, China. pp. 46- 53. (in Chinese) Giovanni C, Maurizio B, Luca M, Laura S, Gian M G, Barbara C, Paola O, Luciano Z, Pier G R. 2004. Blue silver: A very sensitive colloidal coomassie G-250 staining for proteome analysis Electrophoresis, 25, 1327-1333 Gonzalez J C, Banerjee R V, Huang S, Sumner J S, Matthews R G. 1992. Comparison of cobalamin- independent and cobalamin-dependent methionine synthases from Escherichia coli: Two solutions to the same chemical problem. Biochemistry, 31, 6045-6056 Hall A. 1990. The cellular functions of small GTP-binding proteins. Science, 249, 635-640 Hao J H, Dong C J, Zhang Z G, Wang X L, Shang Q M. 2012. Insights into salicylic acid responses in cucumber (Cucumis sativus L.) cotyledons based on a comparative proteomic analysis. Plant Science, 187, 69-82 He L Z, Lu X M, Tian J, Yang Y J, Li B, Li J, Guo S R. 2012. Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots. Proteome Science, 10, 42. Hebeler R, Oeljeklaus S, Reidegeld K A, Eisenacher M, Stephan C, Sitek B, Stühler K, Meyer H E, Sturre M J, Dijkwel P P, Warscheid B. 2008. Study of early leaf senescence in Arabidopsis thaliana by quantitative proteomics using reciprocal 14N/15N labeling and difference gel electrophoresis. Molecular and Cellular Proteomics, 7, 108-120 Houtz R L, Portis Jr A R. 2003. The life of ribulose 1 , 5 - b i s p h o s p h a t e carboxylase/oxygenase- posttranslational facts and mysteries. Archives of Biochemistry and Biophysics, 414, 150. Jones D H, Martin H, Madrazo J, Robinson K A, Nielsen P, Roseboom P H, Patel Y, Howell S A, Aitken A. 1995. Expression and structural analysis of 14-3-3 proteins Journal of Molecular Biology, 245, 375-384.Jorrin J V, Maldonado A M, Castillejo M A. 2007. Plant proteome analysis: A 2006 update. Proteomics, 7, 2947- 2962. Koki K, Saito T. 1985. The recovery and occurrence of bending in cucumber ovary and fruit. Journal of the Japanese Society for Horticultural Science, 54, 357-363 (in Japanese) Koki K, Takashi S. 1984. The occurrence of bending in cucumber fruit and effects of planting density and shading on bending. Journal of the Japanese Society for Horticultural Science, 53, 331-337. (in Japanese) Koki K. 1989. Problems of bending in cucumber fruit growing conditions of bending occurrence. Horticulture and Agriculture, 64, 47-52. (in Japanese) Laporte D C, Stueland C S, Ikeda T P. 1989. Isocitrate dehydrogenasekinase/phosphatase. Biochimie, 71, 1051- 1057. LaPorte D C. 1993. The isocitrate dehydrogenase phosphorylation cycle: Regulation and enzymology. Journal of Cellular Biochemistry, 51, 14-18 Li F, Shi J Y, Shen C F, Chen G G, Hu S P, Chen Y X. 2009. Proteomic characterization of copper stress response in Elsholtzia splendens roots and leaves. Plant Molecular Biology, 71, 251-263 Liu H Y, Qin Z W. 2002. Japanese cucumber grading standards for sales. Yangtze River Vegetable, 2, 10. (in Chinese) Liu Y C, Elly C, Yoshida H. Bonnefoy-Berard N, Altman A. 1996. Activation-modulated association of 14-3- 3 proteins with Cbl in T cells Journal of Biological Chemistry, 271, 14591-14595. Lundqvist T, Schneider G. 1991. Crystal structure of activated ribulose-1,5-bisphosphate carboxylase complexed with its sub strate,ribulose-1,5-bisphosphate Journal of Biological Chemistry, 266, 12604-12611 Ma H. 1994. GTP-binding proteins in plants: New members of an old family. Plant Molecular Biology, 26, 1611- 1636. Ma Q H. 2007. Small GTP-binding proteins and their functions in plants. Journal of Plant Growth Regulation, 26, 369-388 Makino K, Shinagawa H, Nakata A. 1984. Cloning and characterization of the alkaline phosphatase positive regulatory gene (phoM) of Escherichia coli. Molecular Genetics and Genomics, 195, 381-390 Masters S C, Fu H. 2001. 14-3-3 proteins mediate an essential anti-apoptotic signal Journal of Biological Chemistry, 276, 45193-45200 Matthews R G, Goulding C W. 1997. Enzyme-catalyzed methyl transfers to thiols: The role of zinc. Current Opinion in Chemical Biology, 1, 332-339 McMurry J, Begley T. 2005. The Organic Chemistry of Biological Pathways. Roberts and Company Publishers, Colorado, USA. pp. 130-190 Molendijk A J, Ruperti B, Palme K. 2004. Small GTPases in vesicle trafficking. Current Opinion in Plant Biology, 7, 694-700 Muslin A J, Tanner J W, Allen P M, Shaw A S. 1996. Interaction of 14-3-3 with signaling proteins is mediated by phosphoserine Cell, 84, 889-897 Nishikiori M, Mori M, Dohi K, Okamura H, Katoh E, Naito S, Meshi T, Ishikawa M. 2011. A host small GTP- binding protein ARL8 plays crucial roles in tobamovirus RNA replication. PLoS Pathogens, 7, e1002409. O’Farrell. 1975. PH: High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry, 250, 4007. Obsil T, Ghirlando R, Klein D C, Ganguly S, Dyda F. 2001. Crystal structure of the 14-3-3zeta: Serotonin N-acetyltransferase complex a role for scaffolding in enzyme regulation. Cell, 105, 257-267. Rison S C G, Hodgman T C, Thornton J M. 2000. Comparison of functional annotation schemes for genomes. Functional & Integrative Genomics, 1, 56-69 Sano H, Ohashi Y. 1995. Involvement of small GTP-binding proteins in defense signal-transduction pathways of higher plants. Proceedings of the National Academy of Sciences of the United States of America, 92, 4138-4144 Schnarrenberger C, Martin W. 2002. Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants. European Journal of Biochemistry, 269, 868-883 Stepanova A N, Robertson-Hoyt J, Yun J, Benavente L M, Xie D Y, Dole?al K, Schlereth A, Jurgens G, Alonso J M. 2008. TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development. Cell, 133, 177-191 Suliman H S, Appling D R, Robertus J D. 2007. The gene for cobalamin-independent methionine synthase is essential in Candida albicans: A potential antifungal target. Archives of Biochemistry and Biophysics, 467, 218-226 Takahashi H, Iwata T, Kitagawa Y, Takahashi R H, Sato Y, Wakabayashi H, Takashima M, Kido H, Nagashima K, Kenney K, Gibbs Jr C J, Kurata T. 1999. Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt- Jakob disease Clinical and Diagnostic Laboratory Immunology, 6, 983-985 T a u r o g R E, Matthews R G. 2006. Activation of methyltetrahydrofolate by cobalamin-independent methionine synthase. Biochemistry, 45, 5092-5102 Thomas P J, Alexander S, Jan T, Michael G P. 2002. Posttranslational modification of plant plasma membrane H(+)-ATPase as a requirement for functional complementation of a yeast transport mutant. The Journal of Biological Chemistry, 277, 6353-6358 Vernoud V, Horton A C, Yang Z B, Nielsen E. 2003. Analysis of the small GTPase gene superfamily of Arabidopsis. Plant Physiology, 131, 1191-1208 Wilson K A, McManus M T, Gordon M E, Jordan T W. 2002. The proteomics of senescence in leaves of white clover (Trifolium repens L.). Proteomics, 2, 1114-1122 Xiao B, Smerdon S J, Jones D H, Dodson G G, Soneji Y, Aitken A, Gamblin S J. 1995. Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways Nature, 376, 188-191. Xie G S, Liu S K, Tetsuo T, You Z B, Zhang D P. 2002. Cloning and expression of VB12-independent methionine synthase gene responsive to alkaline stress in Rice. Acta Genetica Sinica, 29, 1078-1084. (in Chinese) Xiong X R, Chen W M, Feng S Y, Guo M X, Ai J Y, Wu B. 2003. Simulation on botanic rubisco active center. Chinese Journal of Biochemistry and Molecular Biology, 19, 493-498. (in Chinese) Yoshito K, Hiroshi I, Tohru K, Masato N, Takaya S. 1991. Structure and function of signal transducting GTP binding protein. Annual Review of Biochemistry, 60, 349-400 Zhang P, Qin Z W, Wang L L, Zhou X Y, Wang G L. 2011. Mapping quantitative traits loci linked to bending fruit trait in cucumber. Journal of Northeast Agricultural University (English Edition), 18, 1-5 Zhang P, Qin Z W, Wang L L, Zhou X Y. 2010. Genetic analysis on bent characters of cucumber fruit. Journal of Northeast Agricultural University, 41, 29-33 (in Chinese) Zhang P. 2009. Mapping quantitative traits loci and proteomics studies on bending of cucumber fruit. Ph D thesis, Northeast Agricultural University, China. pp. 35- 54. (in Chinese) |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
