|
|
|
|
|
| Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress |
| Mariama KEBBEH, DONG Jing-xian, HUAN Chen, SHEN Shu-ling, LIU Yan, ZHENG Xiao-lin# |
| College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, P.R.China |
|
|
|
|
摘要
芒果在低温贮藏下常发生冷害(CI)。因此，迫切需要采用先进技术来解决芒果低温胁迫的伤害，提升产品的价值。本文研究了0.1 (MT1)和0.2 mmol L-1 (MT2)褪黑素溶液浸泡30分钟处理对‘凯特’芒果在低温藏期间CI、脯氨酸代谢和相关基因表达的影响。结果表明：与对照相比，MT1浓度褪黑素处理显著降低了芒果果实冷藏期间的CI发展，增加了脯氨酸含量；这些变化伴随着脯氨酸生物合成关键酶活性及其编码基因表达的增加，包括吡啶-5-羧酸合成酶(P5CS)、吡啶-5-羧酸还原酶(P5CR)、鸟氨酸D -氨基转移酶(OAT)、P5CS2、P5CR2和OAT3。同时，经MT1处理芒果的脯氨酸脱氢酶(PDH)活性和PDH3表达量低于对照。因此，褪黑素处理通过调控脯氨酸代谢导致脯氨酸的累积，进而有助于增强‘凯特’芒果果实的抗冷性。
Abstract
Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage. Therefore, advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value. This study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI, proline metabolism, and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control), 0.1 (MT1), and 0.2 mmol L–1 (MT2) melatonin solution for 30 min. The results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the control. These changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis, such as pyrroline-5-carboxylate synthetase (P5CS), pyrroline-5-carboxylate reductase (P5CR), ornithine D-aminotransferase (OAT), P5CS2, P5CR2, and OAT3. Additionally, proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled group. Thus, melatonin treatment has regulated proline metabolism resulting in the accumulation of proline, subsequently contributing to enhancing the chilling tolerance of ‘Keitt’ mango fruit.
|
|
Received: 24 June 2022
Accepted: 14 November 2022
|
| Fund: This research project was financially supported by the National Natural Science Foundation of China (32072280). |
| About author: #Correspondence ZHENG Xiao-lin, E-mail: zheng9393@163.com |
Cite this article:
Mariama KEBBEH, DONG Jing-xian, HUAN Chen, SHEN Shu-ling, LIU Yan, ZHENG Xiao-lin.
2023.
Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress. Journal of Integrative Agriculture, 22(3): 935-944.
|
Aghdam M S, Luo Z, Jannatizadeh A, Sheikh-Assadi M, Sharafi Y, Farmani B, Razavi F. 2019. Employing exogenous melatonin applying confers chilling tolerance in tomato fruits by upregulating ZAT2/6/12, giving rise to promoting endogenous polyamines, proline, and nitric oxide accumulation by triggering arginine pathway activity. Food Chemistry, 275, 549–556.
Arnao M B, Hernández R J. 2014. Melatonin: Plant growth regulator and/or biostimulator during stress. Trends in Plant Science, 19, 789–97.
Arnao M B, Hernández R J. 2020. Melatonin in flowering, fruit set and fruit ripening. Trends in Plant Science, 33, 77–87.
Azim M K, Khan I A, Zhang Y. 2014. Characterisation of mango (Mangifera indica L.) transcriptome and chloroplast genome. Plant Molecular Biology, 85, 193–208.
Bai C M, Wu C, Ma L L, Fu A A, Zheng Y Y, Han J W, Li C B, Yuan S Z, Zheng S F, Gao L P, Zhang X H, Wang Q, Meng D M, Zuo J H. 2023. Transcriptomics and metabolomics analyses provide insights into postharvest ripening and senescence of tomato fruit under low temperature. Horticultural Plant Journal, 9, 109–121.
Bajpai A, Khan K, Muthukumar M, Rajan S, Singh N K. 2018. Molecular analysis of anthocyanin biosynthesis pathway genes and their differential expression in mango peel. Genome, 61, 157–166.
Bhardwaj R, Pareek S, González-Aguilar G A, Domínguez-Avila J A. 2021. Changes in the activity of proline-metabolising enzymes are associated with increased cultivar-dependent chilling tolerance in mangos in response to pre-storage melatonin application. Postharvest Biology and Technology, 182, 111702.
Cao S, Bian K, Shi L, Chung H H, Chen W, Yang Z. 2018. Role of melatonin in cell-wall disassembly and chilling tolerance in cold-stored peach fruit. Journal of Agricultural and Food Chemistry, 66, 5663–5670.
Cao S, Cai Y, Yang Z, Zheng Y. 2012. MeJA induces chilling tolerance in loquat fruit by regulating proline and γ-aminobutyric acid contents. Food Chemistry, 133, 1466–1470.
Dautt-Castro M, Ochoa-Leyva A, Contreras-Vergara C A, Pacheco-Sanchez M A, Casas-Flores S, Sanchez-Flores A, Islas-Osuna M A. 2015. Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening. Frontiers in Plant Science, 6, 62.
Dong J X, Kebbeh M, Yan R, Huan C, Jiang T J, Zheng X L. 2021. Melatonin treatment delays ripening in mangoes associated with maintaining the membrane integrity of fruit exocarp during postharvest. Plant Physiology and Biochemistry, 169, 22–28.
Gao H, Lu Z, Yang Y, Wang D, Yang T, Cao M, Cao W. 2018. Melatonin treatment reduces chilling injury in peach fruit by regulating membrane fatty acid contents and phenolic metabolism. Food Chemistry, 245, 659–666.
Gao H, Zhang Z, Lv X, Cheng N, Peng B, Cao W. 2016. Effect of 24-epibrassinolide on chilling injury of peach fruit in relation to phenolic and proline metabolisms. Postharvest Biology and Technology, 111, 390–397.
Gomes F P, Oliva M A, Mielke M S, Almeida A A F, Aquino L A. 2010. Osmotic adjustment, proline accumulation and cell membrane stability in leaves of Cocos nucifera submitted to drought stress. Scientia Horticulturae, 126, 379–384.
Huan C, Du X, Wang L, Kebbeh M, Li H, Yang X, Zheng X. 2021. Transcriptome analysis reveals the metabolisms of starch degradation and ethanol fermentation involved in alcoholic off-flavour development in kiwifruit during ambient storage. Postharvest Biology and Technology, 180, 111621.
Jannatizadeh A. 2019. Exogenous melatonin applying confers chilling tolerance in pomegranate fruit during cold storage. Scientia Horticulturae, 246, 544–549.
Khan W, Razzak S A, Azim M K. 2019. Comparative transcriptomics of mango (Mangifera indica L.) cultivars provide insights of biochemical pathways involved in flavor and color. bioRxiv, doi: 10.1101/196881.
Kishor P B K, Sangam S, Amruth R N, Laxmi P S, Naidu K R, Rao K R S S, Reddy K J, Theriappan P, Sreenivasulu N. 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Current Science, 88, 424–438.
Kong X M, Ge W Y, Wei B D, Zhou Q, Zhou X, Zhao Y B, Ji S J. 2020. Melatonin ameliorates chilling injury in green bell peppers during storage by regulating membrane lipid metabolism and antioxidant capacity. Postharvest Biology and Technology, 170, 111315.
Li P Y, Zheng X L, Liu Y, Zhu Y Y. 2014. Pre-storage application of oxalic acid alleviates chilling injury in mango fruit by modulating proline metabolism and energy status under chilling stress. Food Chemistry, 142, 72–78.
Liu G, Zhang Y, Yun Z, Hu M, Liu J, Jiang Y, Zhang Z. 2020. Melatonin enhances cold tolerance by regulating energy and proline metabolism in litchi fruit. Foods, 9, 454.
Liu J, Sun J, Pan Y, Yun Z, Zhang Z, Jiang G, Jiang Y. 2021. Endogenous melatonin generation plays a positive role in chilling tolerance in relation to redox homeostasis in litchi fruit during refrigeration. Postharvest Biology and Technology, 178, 111554.
Liu Z L, Li L, Luo Z S, Zeng F F, Jiang L, Tang K C. 2016. Effect of brassinolide on energy status and proline metabolism in postharvest bamboo shoot during chilling stress. Postharvest Biology and Technology, 111, 240–246.
Luo C, He X H, Chen H, Hu Y, Ou S J. 2013. Molecular cloning and expression analysis of four actin genes (MiACT) from mango. Biologia Plantarum, 57, 238–244.
Medina-Santamarina J, Zapata P J, Valverde J M, Valero D, Serrano M, Guillén F. 2021. Melatonin treatment of apricot trees leads to maintaining fruit quality attributes during storage at chilling and non-chilling temperatures. Agronomy, 11, 917.
Nunes M C N, Emond J, Brecht J K, Dea S, Proulx E. 2007. Quality curves for mango fruit (cv. Tommy Atkins and Palmer) stored at chilling and non-chilling temperatures. Journal of Food Quality, 30, 104–120.
dos Santos Neto J P, de Assis M W D, Casagrande I P, Júnior L C C, de Almeida Teixeira G H. 2017. Determination of ‘Palmer’ mango maturity indices using portable near-infrared (VIS-NIR) spectrometer. Postharvest Biology and Technology, 130, 75–80.
Shang H, Cao S, Yang Z, Cai Y, Zheng Y. 2011. Effect of exogenous γ-aminobutyric acid treatment on proline accumulation and chilling injury in peach fruit after long-term cold storage. Journal of Agricultural and Food Chemistry, 59, 1264–1268.
Szabados L, Savouré A. 2010. Proline: A multifunctional amino acid. Trends in Plant Science, 15, 89–97.
Tafolla-Arellano J C, Zheng Y, Sun H, Jiao C, Ruiz-May E, Hernández-Oñate M A, Domozych D. 2017. Transcriptome analysis of mango (Mangifera indica L.) fruit epidermal peel to identify putative cuticle-associated genes. Scientific Reports, 7, 1–13.
Wang S Y, Shi X C, Wang R, Wang H L, Liu F, Laborda P. 2020. Melatonin in fruit production and postharvest preservation: A review. Food Chemistry, 320, 126642.
Wang Y, Luo Z, Huang X, Yang K, Gao S, Du R. 2014. Effect of exogenous γ-aminobutyric acid (GABA) treatment on chilling injury and antioxidant capacity in banana peel. Scientia Horticulturae, 168, 132–137.
Zhang W, Jiang H, Cao J, Jiang W. 2021. Advances in biochemical mechanisms and control technologies to treat chilling injury in postharvest fruits and vegetables. Trends in Food Science & Technology, 113, 355–365.
Zheng X L, Tian S P, Gidley M J, Yue H, Li B Q, Xu Y, Zhou Z W. 2007. Slowing the deterioration of mango fruit during cold storage by pre-storage application of oxalic acid. The Journal of Horticultural Science and Biotechnology, 82, 707–714.
|
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
