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| Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber |
| LIU Li-ying, DUAN Liu-sheng, ZHANG Jia-chang, MI Guo-quan, ZHANG Xiao-lan, ZHANG Zhen-xian, REN Hua-zhong |
1.College of Agronomy and Bio-Technology, China Agricultural University, Beijing 100193, P.R.China
2.Horticulture Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China |
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摘要 Low temperature and high salinity are the major abiotic stresses that restrict cucumber growth and production, breeding materials with multiple abiotic resistance are in greatly need. Here we investigated the effect of introducing the LOS5 gene, a key regulator of ABA biosynthesis in Arabidopsis thaliana, under the stress-responsive RD29A promoter into cucumber (Cucumis sativus L. cv. S516). We found that T1 RD29A-LOS5 transgenic lines have enhanced tolerance to cold and salt stresses. Specifically, transgenic lines exhibited dwarf phenotypes with reduced leaf number, shorter internode, decreased length of the biggest leaf, fewer female flowers, shorter fruit neck and lower vitamin C (Vc). The increased cold tolerance can be reflected from the significantly decreased cold index, the reduced electrolyte leakage index and the MDA content upon cold treatment as compared to those in the control. This may result from the accumulation of internal ABA, soluble sugars and proline, and the enhanced activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the transgenic lines. Under salt treatment, the transgenic lines exhibited increased germination index, vigor index, more lateral roots and increased root fresh weight. Moreover, RD29A-LOS5 transgenic plants displayed quicker responses in salt stress than that in low-temperature stress.
Abstract Low temperature and high salinity are the major abiotic stresses that restrict cucumber growth and production, breeding materials with multiple abiotic resistance are in greatly need. Here we investigated the effect of introducing the LOS5 gene, a key regulator of ABA biosynthesis in Arabidopsis thaliana, under the stress-responsive RD29A promoter into cucumber (Cucumis sativus L. cv. S516). We found that T1 RD29A-LOS5 transgenic lines have enhanced tolerance to cold and salt stresses. Specifically, transgenic lines exhibited dwarf phenotypes with reduced leaf number, shorter internode, decreased length of the biggest leaf, fewer female flowers, shorter fruit neck and lower vitamin C (Vc). The increased cold tolerance can be reflected from the significantly decreased cold index, the reduced electrolyte leakage index and the MDA content upon cold treatment as compared to those in the control. This may result from the accumulation of internal ABA, soluble sugars and proline, and the enhanced activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the transgenic lines. Under salt treatment, the transgenic lines exhibited increased germination index, vigor index, more lateral roots and increased root fresh weight. Moreover, RD29A-LOS5 transgenic plants displayed quicker responses in salt stress than that in low-temperature stress.
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Received: 06 January 2012
Accepted:
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| Fund: This work was supported by the National Basic Research Program of China (973 Program, 2009CB11900) and the National Key Research Program of China (2008BADA6B03, 2008BADB1B05 and 2009BADB8B00). |
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Corresponding Authors:
Correspondence REN Hua-zhong, Tel: +86-10-62731009, Fax: +86-10-62732825, E-mail: renhuazhong@cau.edu.cn
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| About author: LIU Li-ying, E-mail: liuliying35@126.com |
Cite this article:
LIU Li-ying, DUAN Liu-sheng, ZHANG Jia-chang, MI Guo-quan, ZHANG Xiao-lan, ZHANG Zhen-xian, REN Hua-zhong.
2013.
Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber. Journal of Integrative Agriculture, 12(5): 825-834.
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