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Journal of Integrative Agriculture  2025, Vol. 24 Issue (5): 1905-1915    DOI: 10.1016/j.jia.2024.07.027
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Involvement of the cytochrome P450 genes CYP6DW3 and CYP4C64 in afidopyropen resistance in Bemisia tabaci Mediterranean (Q biotype)
Li-jun Ma1*, Juan Tang2*, Qing-he Zhang2, Bing-li Gao2, Cheng Qu2, Ran Wang2#, Chen Luo1#

1 College of Plant Protection, Yangzhou University, Yangzhou 225009, China

2 Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

 Highlights 
The high P450 enzyme activity confers afidopyropen resistance in Bemisia tabaci MED.
CYP6DW3 and CYP4C64 are upregulated in resistant population of B. tabaci MED.
Silencing CYP6DW3 and CYP4C64 increases the susceptibility of B. tabaci adults.
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摘要  

烟粉虱Bemisia tabaci寄主植物广泛,是一种世界性的重要害虫,已对多种杀虫剂产生高抗性。双丙环虫酯是近年商品化的丙烯杀虫剂,其作用方式新颖,选择性,被用于烟粉虱的防治。我们前期对一个烟粉虱田间种群进行抗性筛选,获得了双丙环虫酯高抗性种群(HD-Afi种群。本研究发现在HD-Afi种群中,双丙环虫酯和其他常见化学剂交互抗性小, 但HD-Afi种群的P450酶活性是敏感种群HD-S2.1812个候选P450基因中,CYP6DW3CYP4C64基因在HD-Afi种群中显著上调。RNA干扰CYP6DW3CYP4C64基因显著增加烟粉虱成虫对双丙环虫酯的敏感性,证实两个基因与烟粉虱双丙环虫酯的抗性相关。同源性建模和分子对接分析表明,双丙环虫酯与CYP6DW3CYP4C64的结合稳定,结合自由能分别为-6.87-6.11 kcal mol-1。本研究结果表明CYP6DW3CYP4C64基因的诱导促进了烟粉虱对双丙环虫酯的解毒代谢造成烟粉虱抗性发展。



Abstract  

The tobacco whitefly, Bemisia tabaci, is a notorious pest affecting various crops globally, and it exhibits high levels of resistance to various insecticides.  Afidopyropen is a recently commercialized pyropene insecticide for Btabaci control with high selectivity and a novel mode of action.  We previously identified a high level of afidopyropen resistance in a field-collected population after selection in the lab, and named it the HD-Afi strain.  In the present study, minimal cross-resistance in the HD-Afi strain was found between afidopyropen and other common chemical agents.  However, the P450 enzyme activity in HD-Afi was 2.18 times the level in susceptible strain HD-S.  Expression analysis revealed that two of 12 candidate P450 genes, namely CYP6DW3 and CYP4C64, were significantly up-regulated in HD-Afi.  Silencing CYP6DW3 and CYP4C64 by RNA interference (RNAi) substantially increased the susceptibility of whitefly adults, confirming their involvement in afidopyropen resistance.  Homology modeling and molecular docking analyses demonstrated stable binding of afidopyropen to CYP6DW3 and CYP4C64, with binding free energies of –6.87 and –6.11 kcal mol–1, respectively.  The findings of this study suggest that the induction of CYP6DW3 and CYP4C64 facilitates afidopyropen detoxification, contributing to the development of resistance in Btabaci.

Keywords:  Bemisia tabaci        afidopyropen        insecticide detoxification        cytochrome P450 monooxygenase  
Received: 01 April 2024   Online: 19 July 2024   Accepted: 20 June 2024
Fund: 
This study was partly supported by research grants from the Outstanding Youth Foundation of the Beijing Academy of Agriculture and Forestry Sciences, China (YXQN202301), the National Natural Science Foundation of China (32272522), and the Beijing Natural Science Foundation, China (6232005). 
About author:  Lijun Ma, Tel: +86-514-87978110, E-mail: ma_lijun@foxmail.com; Juan Tang, Tel: +86-514-87978110, E-mail: tangjuan981005@163.com; #Correspondence Chen Luo, Tel: +86-514-87978110, E-mail: Luochen@yzu.edu.cn; Ran Wang, Tel: +86-10-51503338, E-mail: rwang1105@126.com * These authors contributed equally to this study.

Cite this article: 

Lijun Ma, Juan Tang, Qinghe Zhang, Bingli Gao, Cheng Qu, Ran Wang, Chen Luo. 2025. Involvement of the cytochrome P450 genes CYP6DW3 and CYP4C64 in afidopyropen resistance in Bemisia tabaci Mediterranean (Q biotype). Journal of Integrative Agriculture, 24(5): 1905-1915.

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