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| Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus |
| ZHOU Hong, MA Zhe, YUAN Jin , FAN Hong-jie |
| College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China |
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摘要 Suppression subtractive hybridization (SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus (SEZ). There were fourteen genomic regions that only presented in virulent strain ATCC35246. These regions encoded 14 proteins, some of them were homologous to proteins associated with cellular surface structure, molecular synthesis, energy metabolism, regulation, transport systems, and other unknown functions. Primers for 6 particular regions were designed from the already published SEZ sequence. Then, we used PCR to evaluate the distribution and conservation of these 6 DNA fragments in various SEZ strains collected from different sources, regions, groups, and times. The results showed that these 6 DNA fragments were widely distributed in SEZ strains, yet they were not existence in the avirulent strain ST171. Moreover, these fragments could not be detected in other Streptococcus groups.
Abstract Suppression subtractive hybridization (SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus (SEZ). There were fourteen genomic regions that only presented in virulent strain ATCC35246. These regions encoded 14 proteins, some of them were homologous to proteins associated with cellular surface structure, molecular synthesis, energy metabolism, regulation, transport systems, and other unknown functions. Primers for 6 particular regions were designed from the already published SEZ sequence. Then, we used PCR to evaluate the distribution and conservation of these 6 DNA fragments in various SEZ strains collected from different sources, regions, groups, and times. The results showed that these 6 DNA fragments were widely distributed in SEZ strains, yet they were not existence in the avirulent strain ST171. Moreover, these fragments could not be detected in other Streptococcus groups.
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Received: 18 September 2011
Accepted:
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| Fund: This study was supported by the Program for New Century Excellent Talents (NCET) at the University of China (NCET-08-0794), the National Transgenic Major Program (2009ZX08009-154B), the Key Technology Program (R65286D), Jiangsu Province Science and Technology Support Program (BE2009388), the Fundamental Research Funds for the Central Universities, China (KYT 201003), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China. |
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Corresponding Authors:
Correspondence FAN Hong-jie, Tel: +86-25-84395328, E-mail: fhj@njau.edu.cn
E-mail: fhj@njau.edu.cn
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Cite this article:
ZHOU Hong, MA Zhe, YUAN Jin , FAN Hong-jie.
2013.
Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus. Journal of Integrative Agriculture, 12(2): 327-333.
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