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| Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress |
| ZHANG Shuai, LI Jing, LÜ Li-min, WANG Chun-yi, LUO Jun-yu , CUI Jin-jie |
| State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China |
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摘要 Plants reshape their transcriptomes, proteomes and metabolomes in response to insect damage. In this study, we used suppression subtractive hybridization to investigate the transcriptomes of two cotton varieties (CCRI41 and CCRI23) under Apolygus lucorum damage. From the CCRI23 libraries we obtained 92 transcripts and from the CCRI41 libraries we obtained 96 transcripts. 26 and 63 of the transcripts from CCRI23 and CCRI41, respectively, had known functions. Using reverse transcription PCR, we detected expression profile of genes with known functions. Ultimately, we identified eight significantly regulated genes, including one downregulated and four upregulated genes from the CCRI41 libraries, and one downregulated and two upregulated genes from the CCRI23 libraries. Only the gene encoding the polyphenol oxidase (PPO) is involved in plant defense against insect herbivores, and the others are related to improving tolerance to insect damage. Quantitative real-time PCR was used to study changes in expression levels during A. lucorum damage in CCRI23 and CCRI41. Significantly regulated genes from CCRI23 showed a response in CCRI23 but not response in CCRI41. Similarly, significantly regulated genes from CCRI41 showed a response in CCRI41 but not response in CCRI23. The results showed that, among transcriptomes of cotton varieties, there are different responses to A. lucorum damage.
Abstract Plants reshape their transcriptomes, proteomes and metabolomes in response to insect damage. In this study, we used suppression subtractive hybridization to investigate the transcriptomes of two cotton varieties (CCRI41 and CCRI23) under Apolygus lucorum damage. From the CCRI23 libraries we obtained 92 transcripts and from the CCRI41 libraries we obtained 96 transcripts. 26 and 63 of the transcripts from CCRI23 and CCRI41, respectively, had known functions. Using reverse transcription PCR, we detected expression profile of genes with known functions. Ultimately, we identified eight significantly regulated genes, including one downregulated and four upregulated genes from the CCRI41 libraries, and one downregulated and two upregulated genes from the CCRI23 libraries. Only the gene encoding the polyphenol oxidase (PPO) is involved in plant defense against insect herbivores, and the others are related to improving tolerance to insect damage. Quantitative real-time PCR was used to study changes in expression levels during A. lucorum damage in CCRI23 and CCRI41. Significantly regulated genes from CCRI23 showed a response in CCRI23 but not response in CCRI41. Similarly, significantly regulated genes from CCRI41 showed a response in CCRI41 but not response in CCRI23. The results showed that, among transcriptomes of cotton varieties, there are different responses to A. lucorum damage.
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Received: 22 March 2013
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31201518). |
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Corresponding Authors:
CUI Jin-jie, Tel: +86-372-2562217, Fax: +86-372-2562296, E-mail: cuijinjie@126.com
E-mail: cuijinjie@126.com
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| About author: ZHANG Shuai, E-mail: zhsjwl@163.com |
Cite this article:
ZHANG Shuai, LI Jing, Lü Li-min, WANG Chun-yi, LUO Jun-yu , CUI Jin-jie.
2014.
Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress. Journal of Integrative Agriculture, 13(6): 1250-1257.
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| Catala R, Medina J, Salinas J. 2011. Integration of low temperature and light signaling during cold acclimation response in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 108, 16475-16480 Cho E K, Choi Y J. 2009. A nuclear-localized HSP70 confers thermoprotective activity and drought-stress tolerance on plants. Biotechnology Letters, 31, 597-606 Conesa A, Gotz S, Garcia-Gomez J M, Terol J, Talon M, Robles M. 2005. Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics, 21, 3674-3676 Constabel C P, Ryan C A. 1998. A survey of wound- and methyl jasmonate-induced leaf polyphenol oxidase in crop plants. Phytochemistry, 47, 507-511 Deng P, Chen L J, Zhang Z L, Lin K J, Ma W H. 2013. Responses of detoxifying, antioxidant and digestive enzyme activities to host shift of Bemisia tabaci (Hemiptera: Aleyrodidae). Journal of Integrative Agriculture, 12, 296-304 Diatchenko L, Lau Y F, Campbell A P, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E D, Siebert P D. 1996. Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proceedings of the National Academy of Sciences of the United States of America, 93, 6025-6030 Futuyma D J, Agrawal A A. 2009. Macroevolution and the biological diversity of plants and herbivores. Proceedings of the National Academy of Sciences of the United States of America, 106, 18054-18061 Guo X M, Li F G, Guo S D, Zhang Y S, Liu J H, Yao J B, Li G Y, Zhang C J, Yang Y X, Wang Y. 2010. Bivalent insect- resistant gene transgenic cotton variety CCRI 41 with high efficiency and broad aadaptability. Agricultural Sciences in China, 9, http://www.sciencedirect.com/science/article/ pii/S1671292710603136# Heidel A J, Baldwin I T. 2004. Microarray analysis of salicylic acid- and jasmonic acid-signalling in responses of Nicotiana attenuata to attack by insects from multiple feeding guilds. Plant Cell and Environment, 27, 1362- 1373. Hernandez J A, Rubio M, Olmos E, Ros-Barcelo A, Martinez- Gomez P. 2004. Oxidative stress induced by long-term plum pox virus infection in peach (Prunus persica). Physiologia Plantarum, 122, 486-495 Hua H, Lu Q, Cai M, Xu C, Zhou D X, Li X, Zhang Q. 2007. Analysis of rice genes induced by striped stemborer (Chilo suppressalis) attack identified a promoter fragment highly specifically responsive to insect feeding. Plant Molecular Biology, 65, 519-530 Iida H, Kitamura T, Honda K 2009. Comparison of egg- hatching rate, survival rate and development time of the immature stage between B- and Q-biotypes of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) on various agricultural crops. Applied Entomology and Zoology, 44, 267-273 Kessler A, Baldwin I T. 2002. Plant responses to insect herbivory: The emerging molecular analysis. Annual Review of Plant Biology, 53, 299-328 Lau O S, Deng X W. 2012. The photomorphogenic repressors COP1 and DET1: 20 years later. Trends in Plant Science, 17, 584-593 Liu Z F, Yan H C, Wang K B, Kuang T Y, Zhang J P, Gui L L, An X M, Chang W R. 2004. Crystal structure of spinach major light-harvesting complex at 2.72 angstrom resolution. Nature, 428, 287-292 Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods, 25, 402-408 Lu Y H, Qiu F, Feng H Q, Li H B, Yang Z C, Wyckhuys K A G, Wu K M. 2008. Species composition and seasonal abundance of pestiferous plant bugs (Hemiptera: Miridae) on Bt Cotton in China. Crop Protection, 27, 465-472 Lu Y H, Wu K M, Guo Y Y. 2007. Flight potential of Lygus lucorum (Meyer-Dur) (Heteroptera: Miridae). Environmental Entomology, 36, 1007-1013 Mahanil S, Attajarusit J, Stout M J, Thipyapong P. 2008. Overexpression of tomato polyphenol oxidase increases resistance to common cutworm. Plant Science, 174, 456- 466. Mewis I, Tokuhisa J G, Schultz J C, Appel H M, Ulrichs C, Gershenzon J. 2006. Gene expression and glucosinolate accumulation in Arabidopsis thaliana in response to generalist and specialist herbivores of different feeding guilds and the role of defense signaling pathways. Phytochemistry, 67, 2450-2462 Morris E R, Walker J C. 2003. Receptor-like protein kinases: The keys to response. Current Opinion in Plant Biology, 6, 339-342 Nibouche S, Faure G, Kleene P, Ouedraogo S. 1998. First step towards integrated pest management on cotton in Burkina Faso. Crop Protection, 17, 697-701 Reymond P, Weber H, Damond M, Farmer E E. 2000. Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. The Plant Cell, 12, 707-719 Sun Y, Zhang Y J, Cao G C, Gu S H, Wu K M, Gao X W, Guo Y Y. 2011. Rice gene expression profiles responding to larval feeding of the striped stem borer at the 1st to 2nd instar stage. Insect Science, 18, 273-281 Tanaka N, Itoh H, Sentoku N, Kojima M, Sakakibara H, Izawa T, Itoh J I, Nagato Y. 2011. The COP1 ortholog PPS regulates the juvenile-adult and vegetative-reproductive phase changes in rice. The Plant Cell, 23, 2143-2154 Tu L L, Zhang X L, Liu D Q, Jin S X, Cao J L, Zhu L F, Deng F L, Tan J F, Zhang C B. 2007. Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis. Chinese Science Bulletin, 52, 3110-3117 Walling L L. 2000. The myriad plant responses to herbivores. Journal of Plant Growth Regulation, 19, 195-216 Wang J, Constabel C P. 2004. Polyphenol oxidase overexpression in transgenic Populus enhances resistance to herbivory by forest tent caterpillar (Malacosoma disstria). Planta, 220, 87-96 Xu J, Duan X, Yang J, Beeching J R, Zhang P. 2013. Enhanced reactive oxygen species scavenging by overproduction of superoxide dismutase and catalase delays postharvest physiological deterioration of cassava storage roots. Plant Physiology, 161, 1517-1528 Yang H M, Xie S X, Wang L, Jing S L, Zhu X P, Li X W, Zeng W, Yuan H Y. 2011. Identification of up-regulated genes in tea leaves under mild infestation of green leafhopper. Scientia Horticulturae, 130, 476-481 Zhang F, Zhu L, He G C. 2004. Differential gene expression in response to brown planthopper feeding in rice. Journal of Plant Physiology, 161, 53-62 Zhang S A, Zhang Y J, Su H H, Gao X W, Guo Y Y. 2009. Identification and expression pattern of putative odorant- binding proteins and chemosensory proteins in antennae of the microplitis mediator (Hymenoptera: Braconidae). Chemical Senses, 34, 503-512 |
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