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Journal of Integrative Agriculture  2019, Vol. 18 Issue (5): 1093-1102    DOI: 10.1016/S2095-3119(18)62133-1
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
Evaluating the efficacy of an attenuated Streptococcus equi ssp. zooepidemicus vaccine produced by multi-gene deletion in pathogenicity island SeseCisland_4
MA Fang1, WANG Guang-yu2, ZHOU Hong1, MA Zhe1, LIN Hui-xing1, FAN Hong-jie1, 3 
1 MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China
2 National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, P.R.China
3 Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, P.R.China
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Streptococcus equi ssp. zooepidemicus (SEZ) is a pathogen associated with a wild range of animal species.  Frequent outbreaks have occurred in recent years in pigs, horses, goats and dogs which is liable to infect humans.  There is a lack of efficient vaccines against this disease and the occurrence of antibiotic resistance may render drug therapies ineffective.  In this study, gene deletion mutant (ΔSEZ) in pathogenicity islands SeseCisland_4 was constructed.  The mutant ΔSEZ had a 52-fold decrease in 50% lethal dose (LD50) and had less capacity to adhere epithelial cells.  Importantly, immunization of mice with attenuated vaccine ΔSEZ at the dose of 102 colony-forming units (CFU) mL–1 elicited a significant humoral antibody response, with an antibody titer of 1:12 800.  Therefore, 102 CFU mL–1 might be used as the appropriate immune dose for the attenuated vaccine ΔSEZ, which provided mice with efficient protection against virulent SEZ.  In addition, the hyperimmune sera against 102 CFU mL–1 attenuated vaccine ΔSEZ could confer significant protection against virulent SEZ infection in the passive immunization experiment and exhibited efficient bactericidal activity in the whole blood assay.  Meanwhile, no viable bacteria was detected in blood when mice were immunized with ΔSEZ at the dose of 102 CFU mL–1 via hypodermic injection.  Thereafter, the mutant ΔSEZ at the dose of 102 CFU mL–1 could confer significant protection in mice and had less negative effects on host, which could be an effective attenuated vaccine candidate for the prevention of SEZ.
Keywords:  Streptococcus equi ssp. zooepidemicus        zoonosis        attenuated vaccine        immune dose  
Received: 04 June 2018   Accepted:
Fund: This study was supported by the National Key R&D Program of China (2017YFD0500203), the National Natural Science Foundation of China (31672574), the Special Fund for Agro-scientific Research in the Public Interest, China (201403054), the Primary Research & Development Plan of Jiangsu Province, China (BE2017341), the Jiangsu Agricultural Science and Technology Innovation Fund [CX (16) 1028], and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).
Corresponding Authors:  Correspondence FAN Hong-jie, Tel/Fax: +86-25-84396219, E-mail: fhj@njau.   
About author:  MA Fang, E-mail:;

Cite this article: 

MA Fang, WANG Guang-yu, ZHOU Hong, MA Zhe, LIN Hui-xing, FAN Hong-jie. 2019. Evaluating the efficacy of an attenuated Streptococcus equi ssp. zooepidemicus vaccine produced by multi-gene deletion in pathogenicity island SeseCisland_4. Journal of Integrative Agriculture, 18(5): 1093-1102.

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