Please wait a minute...
Journal of Integrative Agriculture  2013, Vol. 12 Issue (1): 103-109    DOI: 10.1016/S2095-3119(13)60210-5
ANIMAL SCIENCE · VETERINARY SCIENCE Advanced Online Publication | Current Issue | Archive | Adv Search |
Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits
 NIU Bu-yue, LAN Xiao-ming, YAN Xiao-hong, DI Sheng-wei, WANG Yang, LI Hai-tao, XIONG Yuanzhu, WANG Xi-biao
1.College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P.R.China
2.Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture/Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/Huazhong Agricultural University, Wuhan 430070, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS).

Abstract  The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS).
Keywords:  pig       MMP-23       molecular cloning       expression       litter size  
Received: 29 November 2011   Accepted:
Fund: 

This study was supported by the Educational Commission of Heilongjiang Province, China (11541031).

Corresponding Authors:  Correspondence WANG Xi-biao, Tel: +86-451-55191109, E-mail: wxibiao1967@yahoo.com.cn     E-mail:  wxibiao1967@yahoo.com.cn
About author:  NIU Bu-yue, E-mail: niubuyue@163.com

Cite this article: 

NIU Bu-yue, LAN Xiao-ming, YAN Xiao-hong, DI Sheng-wei, WANG Yang, LI Hai-tao, XIONG Yuanzhu, WANG Xi-biao. 2013. Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits. Journal of Integrative Agriculture, 12(1): 103-109.

[1]Bennett G L, Leymaster K A. 1989. Integration of ovulationrate, potential embryonic viability and uterine capacityinto a model of litter size in swine. Journal of AnimalScience, 67, 1230-1241

[2]Curry Jr T E, Osteen K G. 2003. The matrix metalloproteinasesystem: changes, regulation, and impact throughoutthe ovarian and uterine reproductive cycle. EndocrineReview, 24, 428-465

[3]Davidson R K, Waters J G, Kevorkian L, Darrah C, CooperA, Donell S T, Clark I M. 2006. Expression profiling ofmetalloproteinases and their inhibitors in synovium andcartilage. Arthritis Research and Therapy, 8, 124-134

[4]Egeblad M, Werb Z. 2002. New functions for the matrixmetalloproteinases in cancer progression. NatureReview Cancer, 2, 161-174

[5]Li K, Ren J, Xing Y, Zhang Z, Ma J, Guo Y, Huang L. 2009.Quantitative trait loci for litter size and prenatal loss ina White Duroc×Chinese Erhualian resource population.Animal Genetics, 40, 963-966

[6]Liu B H. 1998. Statistical Genomics: Linkage, Mappingand QTL Analysis. CRC Press, LLC, USA.Ohnishi J, Ohnishi E, Jin M, Hirano W, Nakane D, MatsuiH, Kimura A, Sawa H, Nakayama K, Shibuya H, et al.2001. Cloning and characterization of a rat ortholog ofMMP-23 (matrix metalloproteinase-23), a unique typeof membrane-anchored matrix metalloproteinase andconditioned switching of its expression during theovar ian fol l i cular deve lopment  MolecularEndocrinology, 15, 747-764

[7]Ohnishi J, Ohnishi E, Shibuya H, Takahashi T. 2005.Functions for proteinases in the ovulatory process.Biochimical et Biophysica Acta (Proteins andProteomics), 1751, 95-109

[8]Ohnishi J, Ohnishi E, Takahashi T. 2004. The possibleinvolvement of matrix metalloproteinase-23 (MMP-23)activity in ovulatory processes and conditionedswitching of its expression during folliculardevelopment In: Proceedings of 37th Annual Meetingof the Society for the Study of Reproduction. Vancouver,BC, Canada.

[9]Onteru S K, Ross J W, Rothschild M F. 2009. The role ofgene discovery, QTL analyses and gene expression inreproductive traits in the pig. Society ReproductionFertility Supplement, 66, 87-102

[10]Soller M. 1994. Marker assisted selection - an overview.Animal Biotechnology, 5, 193-207

[11]Sun X, Mei S, Tao H, Wang G, Su L, Jiang S, Deng C, XiongY, Li F. 2011. Microarray profiling for differential geneexpression in PMSG-hCG stimulated preovulatoryovarian follicles of Chinese Taihu and LargeWhite sows.BMC Genomics, 12, 111.

[12]Velasco G, Pendás A M, Fueyo A, Knäuper V, Murphy G,López-Otín C. 1999. Cloning and characterization ofhuman MMP-23, a new matrix metalloproteinasepredominantly expressed in reproductive tissues andlacking conserved domains in other family members Journal of Biological Chemistry, 274, 4570-4576

[13]Wilkie P J, Paszek AA, Beattie C W, Alexander L J,WheelerM B, Schook L B. 1999. A genomic scan of porcinereproductive traits reveals possible quantitative traitloci (QTLs) for number of corpora lutea. MammalianGenome, 10, 573-578

[14]Xiong Y Z. 1999. Pig Biochemistry and MolecularGenetics: Experimental Introduction. ChineseAgriculture Press, Beijing. (in Chinese)Yong L D, Leymaster K A, Christenson R K. 1996.Opportunities for indirect selection for uterine capacityof swine. Journal of Animal Science, 74, 119-123
[1] Jialing Fu, Qingjiang Wu, Xia Wang, Juan Sun, Li Liao, Li Li, Qiang Xu. A novel histone methyltransferase gene CgSDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2633-2648.
[2] Lin Chen, Chao Li, Jiahao Zhang, Zongrui Li, Qi Zeng, Qingguo Sun, Xiaowu Wang, Limin Zhao, Lugang Zhang, Baohua Li. Physiological and transcriptome analyses of Chinese cabbage in response to drought stress[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2255-2269.
[3] Yutong Zhang, Hangwei Liu, Song Cao, Bin Li, Yang Liu, Guirong Wang.

Identification of transient receptor potential channel genes and functional characterization of TRPA1 in Spodoptera frugiperda  [J]. >Journal of Integrative Agriculture, 2024, 23(6): 1994-2005.

[4] YANG Wei-bing, ZHANG Sheng-quan, HOU Qi-ling, GAO Jian-gang, WANG Han-Xia, CHEN Xian-Chao, LIAO Xiang-zheng, ZHANG Feng-ting, ZHAO Chang-ping, QIN Zhi-lie.

Transcriptomic and metabolomic analysis provides insights into lignin biosynthesis and accumulation and differences in lodging resistance in hybrid wheat [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1105-1117.

[5] Liyao Su, Min Wu, Tian Zhang, Yan Zhong, Zongming (Max) Cheng.

Identification of key genes regulating the synthesis of quercetin derivatives in Rosa roxburghii through integrated transcriptomics and metabolomics [J]. >Journal of Integrative Agriculture, 2024, 23(3): 876-887.

[6] Jun Zhou, Qing Lin, Xueyan Feng, Duanyang Ren, Jinyan Teng, Xibo Wu, Dan Wu, Xiaoke Zhang, Xiaolong Yuan, Zanmou Chen, Jiaqi Li, Zhe Zhang, Hao Zhang.

Evaluating the performance of genomic selection on purebred population by incorporating crossbred data in pigs [J]. >Journal of Integrative Agriculture, 2024, 23(2): 639-648.

[7] Caiyun Liu, Lulu Wu, Shuyue Fan, Yongsheng Tao, Yunkui Li.

The protective effect of cyclodextrin on the color quality and stability of Cabernet Sauvignon red wine [J]. >Journal of Integrative Agriculture, 2024, 23(1): 310-323.

[8] Akmaral Baidyussen, Gulmira Khassanova, Maral Utebayev, Satyvaldy Jatayev, Rystay Kushanova, Sholpan Khalbayeva, Aigul Amangeldiyeva, Raushan Yerzhebayeva, Kulpash Bulatova, Carly Schramm, Peter Anderson, Colin L. D. Jenkins, Kathleen L. Soole, Yuri Shavrukov. Assessment of molecular markers and marker-assisted selection for drought tolerance in barley (Hordeum vulgare L.)[J]. >Journal of Integrative Agriculture, 2024, 23(1): 20-38.
[9] Mu Zeng, Binhu Wang, Lei Liu, Yalan Yang, Zhonglin Tang. Genome-wide association study identifies 12 new genetic loci associated with growth traits in pigs[J]. >Journal of Integrative Agriculture, 2024, 23(1): 217-227.
[10] Atiqur RAHMAN, Md. Hasan Sofiur RAHMAN, Md. Shakil UDDIN, Naima SULTANA, Shirin AKHTER, Ujjal Kumar NATH, Shamsun Nahar BEGUM, Md. Mazadul ISLAM, Afroz NAZNIN, Md. Nurul AMIN, Sharif AHMED, Akbar HOSAIN. Advances in DNA methylation and its role in cytoplasmic male sterility in higher plants[J]. >Journal of Integrative Agriculture, 2024, 23(1): 1-19.
[11] ZHANG Li-hua, ZHU Ling-cheng, XU Yu, LÜ Long, LI Xing-guo, LI Wen-hui, LIU Wan-da, MA Feng-wang, LI Ming-jun, HAN De-guo. Genome-wide identification and function analysis of the sucrose phosphate synthase MdSPS gene family in apple[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2080-2093.
[12] XU Kui, ZHOU Yan-rong, SHANG Hai-tao, XU Chang-jiang, TAO Ran, HAO Wan-jun, LIU Sha-sha, MU Yu-lian, XIAO Shao-bo, LI Kui. Pig macrophages with site-specific edited CD163 decrease the susceptibility to infection with porcine reproductive and respiratory syndrome virus[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2188-2199.
[13] LIAO Zhen-qi, DAI Yu-long, WANG Han, Quirine M. KETTERINGS, LU Jun-sheng, ZHANG Fu-cang, LI Zhi-jun, FAN Jun-liang. A double-layer model for improving the estimation of wheat canopy nitrogen content from unmanned aerial vehicle multispectral imagery[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2248-2270.
[14] HOU Qian-dong, HONG Yi, WEN Zhuang, SHANG Chun-qiong, LI Zheng-chun, CAI Xiao-wei, QIAO Guang, WEN Xiao-peng. Molecular characterization of the SAUR gene family in sweet cherry and functional analysis of PavSAUR55 in the process of abscission[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1720-1739.
[15] XIE Lei, QIN Jiang-tao, RAO Lin, CUI Deng-shuai, TANG Xi, XIAO Shi-jun, ZHANG Zhi-yan, HUANG Lu-sheng. Effects of carcass weight, sex and breed composition on meat cuts and carcass trait in finishing pigs[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1489-1501.
No Suggested Reading articles found!