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| ASimpleMethod for the Isolation andPurification of 2,4-Dihydroxy-7-Methoxy- 2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings |
| LI Jing, LIU Xin-gang, DONG Feng-shou, XU Jun, GUO Li-qun, KONG Zhi-qiang, TIAN Ying-ying, WU Yan-bin , ZHENG Yong-quan |
1.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2.Henan Institute of Science and Technology, Xinxiang 453003, P.R.China |
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摘要 2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), the dominant benzoxazinoid hydroxamic acid in maize (Zea Mays L.), serves as important factors of resistance against insects and microbial diseases, allelochemicals used in competition with other plants. In this paper, a novel and simple method for the isolation and purification of DIMBOA from maize seedlings was developed. Frozen shoots from 7-d-old maize seedlings (1 000×g) were firstly defrosted and then were directly homogenized and extracted with ethyl acetate. The macerate was allowed to stand at room temperature (25±2)°C for 1 h to allow enzymatic release of DIMBOA from DIMBOA-glucoside. Then the ethyl acetate phase was filtered, dried and evaporated to dryness. The resulting light-tan, semicrystalline residue was stored at -20°C for 24 h. Upon recrystallization from acetone-hexane, a relative higher yield (0.58 g) of pure DIMBOA crystals was obtained compared with the yield afforded by Woodward methodology (0.26 g).
Abstract 2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), the dominant benzoxazinoid hydroxamic acid in maize (Zea Mays L.), serves as important factors of resistance against insects and microbial diseases, allelochemicals used in competition with other plants. In this paper, a novel and simple method for the isolation and purification of DIMBOA from maize seedlings was developed. Frozen shoots from 7-d-old maize seedlings (1 000×g) were firstly defrosted and then were directly homogenized and extracted with ethyl acetate. The macerate was allowed to stand at room temperature (25±2)°C for 1 h to allow enzymatic release of DIMBOA from DIMBOA-glucoside. Then the ethyl acetate phase was filtered, dried and evaporated to dryness. The resulting light-tan, semicrystalline residue was stored at -20°C for 24 h. Upon recrystallization from acetone-hexane, a relative higher yield (0.58 g) of pure DIMBOA crystals was obtained compared with the yield afforded by Woodward methodology (0.26 g).
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Received: 26 December 2011
Accepted:
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| Fund: This research was financially supported by the National Natural Science Foundation of China (30900951). |
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Corresponding Authors:
Correspondence ZHNEG Yong-quan, Tel/Fax: +86-10-62815908, E-mail: yongquan_zheng@yahoo.com.cn
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| About author: LI Jing, Mobile: 18222914598, E-mail: lijing2011@gmail.com |
Cite this article:
LI Jing, LIU Xin-gang, DONG Feng-shou, XU Jun, GUO Li-qun, KONG Zhi-qiang, TIAN Ying-ying, WU Yan-bin , ZHENG Yong-quan.
2013.
ASimpleMethod for the Isolation andPurification of 2,4-Dihydroxy-7-Methoxy- 2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings. Journal of Integrative Agriculture, 12(1): 95-102.
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