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Journal of Integrative Agriculture  2012, Vol. 12 Issue (10): 1644-1653    DOI: 10.1016/S1671-2927(00)8697
PHYSIOLOGY & BIOCHEMISTRY · TILLAGE · CULTIVATION Advanced Online Publication | Current Issue | Archive | Adv Search |
Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.
 LIU Jing, HOU Zhi-hui, LIU Guo-hua, HOU Li-xia,  LIU Xin
Key Laboratory of Plant Biotechnology in Universities of Shandong Province/College of Life Science, Qingdao Agricultural University,Qingdao 266109, P.R.China
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摘要  Pharmacological, laser scanning confocal microscopic (LSCM), and spectrophotographic approaches were used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement in response to ethylene in Vicia faba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase (NR) activity in guard cells or leaves of V. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/Dcysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.

Abstract  Pharmacological, laser scanning confocal microscopic (LSCM), and spectrophotographic approaches were used to study the roles of hydrogen sulfide (H2S) and nitric oxide (NO) in signaling transduction of stomatal movement in response to ethylene in Vicia faba L. Ethylene treatment resulted in the dose-dependent stomatal closure under light, and this effect was blocked by the inhibitors of H2S biosynthesis in V. faba L. Additionally, ethylene induces H2S generation and increases L-/D-cysteine desulfhydrase (pyridoxalphosphate-dependent enzyme) activity in leaves of V. faba L. Inhibitors of H2S biosynthesis have no effect on the ethylene-induced stomatal closure, NO accumulation, and nitrate reductase (NR) activity in guard cells or leaves of V. faba L. Moreover, the ethylene-induced increase of H2S levels and L-/Dcysteine desulfhydrase activity declined when NO generation was inhibited. Therefore, we conclude that H2S and NO probably are involved in the signal transduction pathway of ethylene-induced stomatal closure. H2S may represent a novel component downstream of NO in the ethylene-induced stomatal movement in V. faba L.
Keywords:  hydrogen sulfide       nitric oxide       ethylene       stomatal closure       Vicia faba L.  
Received: 21 October 2011   Accepted:
Fund: 

This study was supported by the National Natural Science Foundation of China (30970288 and 31170237), the National Science Foundation of Shandong Province, China (ZR2010CM024), and the Foundation of State Key Laboratory of Plant Physiology and Biochemistry, China (SKLPPBKF11001).

Corresponding Authors:  Correspondence LIU Xin, Tel: +86-532-88030224, E-mail: liuxin6080@yahoo.com.cn     E-mail:  liuxin6080@yahoo.com.cn

Cite this article: 

LIU Jing, HOU Zhi-hui, LIU Guo-hua, HOU Li-xia, LIU Xin. 2012. Hydrogen Sulfide May Function Downstream of Nitric Oxide in Ethylene- Induced Stomatal Closure in Vicia faba L.. Journal of Integrative Agriculture, 12(10): 1644-1653.

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