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| Comparative Study on the Expression of Genes Involved in Carotenoid and ABA Biosynthetic Pathway in Response to Salt Stress in Tomato |
| DUAN Hui-kun, ZHU Yan, LI Wen-long, HUA Xue-jun, LIU Yong-xiu, DENG Xin |
1.Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China
2.Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R.China
3.Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China |
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摘要 1 Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R.China 3 Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China Carotenoid biosynthetic pathway produces not only pigments that protect photosynthetic system against photo-oxidative damage, but also precursors of abscisic acid, the major hormone regulates stress responses. To understand the response of carotenoid biosynthetic pathway to salt stress, the expression of the genes involved in carotenoid and ABA biosynthesis were compared in cultivated tomato Solanum lycopersicon cv. Moneymaker and its relative wild genotype S. pimpinellifolium (PI365967) together with the contents of carotenoids and ABA. The results showed that 11 of the 15 genes investigated were up-regulated and four unaltered in Moneymaker after 5 h of salt stress; whereas only four genes were up-regulated, four unaltered, and seven down-regulated in PI365967 after stress. Further comparison revealed that 11 salinity-induced genes were expressed significantly lower in Moneymaker than in PI365967 under normal condition, and 8 of them were induced to similar levels after salt stress. In consistence, ABA level was doubled in Moneymaker but kept consistent in PI365967 after salt stress, though the contents of neoxanthin, violaxanthin, β-carotene, lutein, and total carotenoids were kept unchanged in both species. Since it is known that PI365967 is more tolerant to salt stress than Moneymaker, we proposed that the constitutive high level of carotenoid and ABA biosynthetic pathway under normal growth condition could be benefit to PI365967 for establishing the early response to salt stress. In addition, CrtR-b1 and CrtR-b2 that encode β-carotenoid hydroxylases were the only genes in carotenoid biosynthetic pathway that were up-regulated by salt stress in both species. The CrtR-b2 gene was cloned from both species and no essential difference was found in the encoded amino acid sequences. Transformation of CrtR-b2 to tobacco improved the seed germination under salt stress condition, indicating that the hydrolysis of β-carotenoid is the target of transcriptional regulation of the carotenoid biosynthesis in both tomato cultivar and wild relative.
Abstract 1 Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R.China 3 Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China Carotenoid biosynthetic pathway produces not only pigments that protect photosynthetic system against photo-oxidative damage, but also precursors of abscisic acid, the major hormone regulates stress responses. To understand the response of carotenoid biosynthetic pathway to salt stress, the expression of the genes involved in carotenoid and ABA biosynthesis were compared in cultivated tomato Solanum lycopersicon cv. Moneymaker and its relative wild genotype S. pimpinellifolium (PI365967) together with the contents of carotenoids and ABA. The results showed that 11 of the 15 genes investigated were up-regulated and four unaltered in Moneymaker after 5 h of salt stress; whereas only four genes were up-regulated, four unaltered, and seven down-regulated in PI365967 after stress. Further comparison revealed that 11 salinity-induced genes were expressed significantly lower in Moneymaker than in PI365967 under normal condition, and 8 of them were induced to similar levels after salt stress. In consistence, ABA level was doubled in Moneymaker but kept consistent in PI365967 after salt stress, though the contents of neoxanthin, violaxanthin, β-carotene, lutein, and total carotenoids were kept unchanged in both species. Since it is known that PI365967 is more tolerant to salt stress than Moneymaker, we proposed that the constitutive high level of carotenoid and ABA biosynthetic pathway under normal growth condition could be benefit to PI365967 for establishing the early response to salt stress. In addition, CrtR-b1 and CrtR-b2 that encode β-carotenoid hydroxylases were the only genes in carotenoid biosynthetic pathway that were up-regulated by salt stress in both species. The CrtR-b2 gene was cloned from both species and no essential difference was found in the encoded amino acid sequences. Transformation of CrtR-b2 to tobacco improved the seed germination under salt stress condition, indicating that the hydrolysis of β-carotenoid is the target of transcriptional regulation of the carotenoid biosynthesis in both tomato cultivar and wild relative.
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Received: 03 March 2011
Accepted:
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| Fund: This work was supported by the Knowledge Innovation Key Program of the Chinese Academy of Sciences (KSCXZYW- N-013). |
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Corresponding Authors:
DENG Xin, Tel: +86-10-62836261, Fax: +86-10-62836936, E-mail: deng@ibcas.ac.cn
E-mail: deng@ibcas.ac.cn
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| About author: DUAN Hui-kun, E-mail: duanhk001@126.com; |
Cite this article:
DUAN Hui-kun, ZHU Yan, LI Wen-long, HUA Xue-jun, LIU Yong-xiu, DENG Xin.
2012.
Comparative Study on the Expression of Genes Involved in Carotenoid and ABA Biosynthetic Pathway in Response to Salt Stress in Tomato. Journal of Integrative Agriculture, 12(7): 1093-1102.
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| [1]Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410. [2]Bartley G E, Scolnik P A. 1995. Plant carotenoids: pigments for photoprotection, visual attraction, and human health. The Plant Cell, 7, 1027-1038. [3]Chaudhary N, Nijhawan A, Khurana J P, Khurana P. 2010. Carotenoid biosynthesis genes in rice: structural analysis, genome-wide expression profiling and phylogenetic analysis. Molecular of Genetics and Genomics, 283, 13-33. [4]Chenna R, Sugawara H, Koike T, Lopez R, Gibson T J, Higgins D G, Thompson J D. 2003. Multiple sequence alignment with the clustal series of programs. Nucleic Acids Research, 31, 3497-3500. [5]Chomczynski P, Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Analytical Biochemistry, 162, 156-159. [6]Davison P A, Hunter C N, Horton P. 2002. Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature, 418, 203-206. [7]DellaPenna D, Pogson B J. 2006. Vitamin synthesis in plants: tocopherols and carotenoids. Annual Review of Plant Biology, 57, 711-738. [8]Demmig-Adams B, Gilmore A M, Adams W W. 1996. Carotenoids 3: in vivo function of carotenoids in higher plants. FASEB Journal, 10, 403-412. [9]Doyle J J, Doyle J L. 1990. Isolation of plant DNA from fresh tissue. Focus, 12, 13-15. [10]Fang J, Chai C, Qian Q, Li C, Tang J, Sun L, Huang Z, Guo X, Sun C, Liu M. 2008. Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice. The Plant Journal, 54, 177-189. [11]Fang J, Chu C. 2008. Abscisic acid and the pre-harvest sprouting in cereals. Plant Signal and Behavior, 3, 1046-1048. [12]Fray R, Wallace A, Praser P D. 1995. Constitutive expression of a fruit phytoene synthase gene in transgenic tomatoes cause 8 dwarfism by redirecting metabolites from the gibberellin pathway. The Plant Journal, 8, 693-701. [13]Frisch D A, Harris-Haller L W, Yokubaitis N T, Thomas T L, . 2012, CAAS. All rights reserved. Published by Elsevier Ltd. Hardin S H, Hall T C. 1995. Complete sequence of the binary vector bin 19. Plant Molecular Biology, 27, 405-409. [14]Galpaz N, Ronen G, Khalfa Z, Zamir D, Hirschberg J. 2006. A chromoplast-specific carotenoid biosynthesis pathway is revealed by cloning of the tomato whiteflower locus. The Plant Cell, 18, 1947-1960. [15]Galpaz N, Wang Q, Menda N, Zamir D, Hirschberg J. 2008. Abscisic acid deficiency in the tomato mutant highpigment 3 leading to increased plastid number and higher fruit lycopene content. The Plant Journal, 53, 717-730. [16]Hirschberg J. 2001. Carotenoid biosynthesis in flowering plants. Current Opinion in Plant Biology, 4, 210-218. [17]Hoagland D R, Arnon D I. 1950. The water-culture method for growing plants without soil. In: California Agricultural Experiment Station Circular 347. College of Agriculture, University of California, Berkeley, CA, USA. pp. 1-39. [18]Horsh R B, Fry J E, Hoffmann N L, Eichholtz D, Rogers S G, Fraley R T. 1985. A simple and general method for transferring genes into plants. Science, 227, 1229-1231. [19]Hsu Y T, Kao C H. 2003. Role of abscisic acid in cadmium tolerance of rice (Oryza sativa L.) seedlings. Plant Cell and Environment, 26, 867-874. [20]Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K. 2001. Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis. The Plant Journal, 27, 325-333. [21]Li Z, Ahn T K, Avenson T J, Ballottari M, Cruz J A, Kramer D M, Bassi R, Fleming G R, Keasling J D, Niyogi K K. 2009. Lutein accumulation in the absence of zeaxanthin restores nonphotochemical quenching in the Arabidopsis thaliana npq1 mutant. The Plant Cell, 21, 1798-1812. [22]Lichtenthaler H K, Schwender J, Disch A, Rohmer M. 1997. Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathway. FEBS Letters, 400, 271-274. [23]Olson J A, Krinsky N I. 1995. Introduction: the colorful, fascinating world of the carotenoids: important physiologic modulators. FASEB Journal, 9, 1547-1550. [24]Park H, Kreunen S S, Cuttriss A J, DellaPenna D, Pogson B J. 2002. Identification of the carotenoid isomerase provides insight into carotenoid biosynthesis, prolamellar body formation, and photomorphogenesis. The Plant Cell, 14, 321-332. [25]Pogson B J, Rissler H M. 2000. Genetic manipulation of carotenoid biosynthesis and photoprotection. Philosophical Transactions of the Royal Society (B), 355, 1395-1403. [26]Qin X, Zeevaart J A. 1999. The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean. Proceedings of the National Academy of Sciences of the United States of America, 96, 15354-15361. [27]Rock C D, Zeevaart J A. 1991. The aba mutant of Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 88, 7496-7499. [28]Sun W, Xu X, Zhu H, Liu A, Liu L, Li J, Hua X. 2010. Comparative transcriptomic profiling of salt tolerant wild tomato species and salt sensitive tomato cultivar. Plant and Cell Physiology, 51, 997-1006. [29]Taji T, Seki M, Satou M, Sakurai T, Kobayashi M, Ishiyama K, Narusaka Y, Narusaka M, Zhu J K, Shinozaki K. 2004. Comparative genomics in salt tolerance between Arabidopsis and Arabidopsis-related halophyte salt cress using Arabidopsis Microarray. Plant Physiology, 135, 1697-1709. [30]Tan B, Schwartz S, Zeevaart J, McCarty D. 1997. Genetic control of abscisic acid biosynthesis in maize. Proceedings of the National Academy of Sciences of the United States of America, 94, 12235-12240. [31]Thayer S, Bjorkman O. 1990. Leaf xanthophyll content and composition in sun and shade determined by HPLC. Photosynthesis Research, 23, 331-343. [32]Tian L, DellaPenna D. 2004. Progress in understanding the origin and functions of carotenoid hydroxylases in plants. Archives of Biochemistry and Biophysics, 430, 22-29. [33]Tian L, Magallanes-Lundback M, Musetti V, DellaPenna D. 2003. Functional analysis of beta-and epsilon-ring carotenoid hydroxylases in Arabidopsis. The Plant Cell, 15, 1320-1332. [34]Volkov V, Wang B, Dominy P, Fricke W, Amtmann A. 2004. Thellungiella halophila, a salt-tolerant relative of Arabidopsis thaliana, possesses effective mechanisms to discriminate between potassium and sodium. Plant, Cell and Environment, 27, 1-14. [35]Wang N, Fang W, Han H, Sui N, Li B, Meng Q W. 2008. Overexpression of zeaxanthin epoxidase gene enhances the sensitivity of tomato PSII photoinhibition to high light and chilling stress. Physiologia Plantarum, 132, 384-396. [36]Welsch R, Wust F, Bar C, Al-Babili S, Beyer P. 2008. A third phytoene synthase is devoted to abiotic stress-induced abscisic acid formation in rice and defines functional diversification of phytoene synthase genes. Plant Physiology, 147, 367-380. [37]Wong C E, Li Y, Labbe A, Guevara D, Nuin P, Whitty B, Diaz C, Golding G B, Gray G R, Weretilnyk E A, et al. 2006. Transcriptional profiling implicates novel interactions between abiotic stress and hormonal responses in Thellungiella, a close relative of Arabidopsis. Plant Physiology, 140, 1437-1450. [38]Xiong L, Lee H, Ishitani M, Zhu J K. 2002. Regulation of osmotic stress-responsive gene expression by the LOS6/ABA1 locus in Arabidopsis. The Journal of Biological Chemistry, 277, 8588-8596. [39]Yang J, Guo Z. 2007. Cloning of a 9-cis-epoxycarotenoid dioxygenase gene (SgNCED1) from Stylosanthes guianensis and its expression in response to abiotic stresses. Plant Cell Reports, 26, 1383-1390. |
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