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| Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum |
| CHEN Shuai, LIU Guan-shan, WANG Yuan-ying, SUN Yu-he , CHEN Jia |
1. Key Laboratory of Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences,Qingdao 266101, P.R.China
2. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China |
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摘要 Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca2+ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRTPCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.
Abstract Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca2+ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRTPCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses.
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Received: 15 July 2010
Accepted:
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| Fund: This study was supported in part by the Special Grand Science and Technology Projects for China National Tobacco Corporation (110200701022, 110200902036), China and the open subject from the State Key Laboratory of Plant Physiology and Biochemistry (PPB08004). |
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Corresponding Authors:
Correspondence LIU Guan-shan, Tel: +86-532-88703168, Fax: +86-532-88703168, E-mail: liuguanshan2002@163.com
E-mail: liuguanshan2002@163.com
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Cite this article:
CHEN Shuai, LIU Guan-shan, WANG Yuan-ying, SUN Yu-he , CHEN Jia.
2011.
Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum. Journal of Integrative Agriculture, 10(12): 1851-1860.
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