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Journal of Integrative Agriculture
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Degradation Effects on Dichlorvos by a Biocontrol Strain, Trichoderma atroviride T23
SUN Jia-nan1, 2*, SI Gao-yue1, 2*, LIU Hong-yi1, 2, LI Ya-qian1, 2, WANG Xin-hua1, 2CHEN Jie1, 2

1 School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, P.R.China

2 The State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, P.R.China

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Abstract  Excessive use of organophosphate pesticides (OP), such as dichlorvos, in farming system poses a threat to human health through potential contamination of environment. To date, biodegradation has been prospected most promising approach to eliminate environmental OP residues. Trichoderma species as a biological control microorganism is often exposed to the chemical pesticides applied in environments, so it is necessary to understand the mechanism of degradation of dichlorvos by Trichoderma. In this study, dichlorvos significantly inhibited the growth, sporulation and pigmentation of T. atroviride T23, and the dichlorvos degradation activity of T23 required the initial induction effect of dichlorvos and the culture conditions, including the nutrient and pH values of the medium. Various changed primary and secondary metabolites released from T23 in the presence of dichlorvos were speculated as the energy and antioxidants for the strain itself to tolerate dichlorvos stress. The results showed that T23 could produce a series of enzymes, especially the intracellular enzymes, to degrade dichlorvos. The activities of the intracellular enzyme generated by T23 were differentially changed along time course and especially relied on initial dichlorvos concentration, ammonium sulfate and phosphate added in the medium. In conclusion, some dichlorvos-induced chemical degradation related enzymes of T23 were proved to be involved in the degradation of dichlorvos.
Keywords:  Trichoderma atroviride T23       dichlorvos        intracellular enzyme        induced enzyme activity  
Online: 24 January 2023  
Fund: This work was supported by the National Natural Science Foundation of China (31872015), Shanghai Science and Technology Innovation Action Program of the Shanghai Science and Technology Commission (21N41900200), Shanghai Agriculture Applied Technology Development Program (2022-02-08-00-12-F0-01143), the China Agriculture Research System of MOF and MAR, the National Key R&D Program of China 2017YFD0200403.
About author:  SUN Jia-nan, E-mail:; SI Gao-yue, E-mail:; Correspondence CHEN Jie, Tel: +86-21-34206141, E-mail:

Cite this article: 

SUN Jia-nan, SI Gao-yue, LIU Hong-yi, LI Ya-qian, WANG Xin-hua, CHEN Jie. 2023. Degradation Effects on Dichlorvos by a Biocontrol Strain, Trichoderma atroviride T23. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2023.01.009

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