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| Expression Analysis of Restorer Alleles-Induced Genes in Pepper |
| Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University |
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摘要 Fertility restoration of cytoplasmic male-sterility in pepper (Capsicum annuum L.) is useful for commercial production ofhybrid seeds. However, the mechanism of fertility restoration has not been determined. We previously constructed acDNA library and identified some genes related to fertility restoration in pepper using suppression subtractive hybridizationtechnology. In this study, the expression patterns of 20 genes were investigated using semi-quantitative RT-PCR. Threegenes expressed only in restorer lines, but not in sterility lines. Four genes expressed only in anther, but not in otherorgans. Among these 7 genes, the clone TG31 was observed to specifically express in anther of restorer lines. The workdescribed here provides a comprehensive overview on the expression pattern of the genes that are induced by restoreralleles in pepper. It will also contribute to the current understanding of molecular networks for the regulation of fertilityrestoration.
Abstract Fertility restoration of cytoplasmic male-sterility in pepper (Capsicum annuum L.) is useful for commercial production ofhybrid seeds. However, the mechanism of fertility restoration has not been determined. We previously constructed acDNA library and identified some genes related to fertility restoration in pepper using suppression subtractive hybridizationtechnology. In this study, the expression patterns of 20 genes were investigated using semi-quantitative RT-PCR. Threegenes expressed only in restorer lines, but not in sterility lines. Four genes expressed only in anther, but not in otherorgans. Among these 7 genes, the clone TG31 was observed to specifically express in anther of restorer lines. The workdescribed here provides a comprehensive overview on the expression pattern of the genes that are induced by restoreralleles in pepper. It will also contribute to the current understanding of molecular networks for the regulation of fertilityrestoration.
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Received: 28 July 2010
Accepted:
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Corresponding Authors:
Correspondence SHEN Huo-lin, Tel: +86-10-62732831, E-mail: SHL1606@cau.edu.cn
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| About author: GUO Shuang, Ph D, E-mail: guoshuang6286757@163.com |
Cite this article:
GUO Shuang, MA Ning, YANG Wen-cai, SUN Yu-jie, SHEN Huo-lin.
2011.
Expression Analysis of Restorer Alleles-Induced Genes in Pepper. Journal of Integrative Agriculture, 10(7): 1010-1015.
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