Identification and transfer of resistance to Fusarium head blight from Elymus repens chromosome arm 7StL into wheat

doi:10.1016/j.jia.2024.03.027

Journal of Integrative Agriculture

2025, Vol. 24

Issue (11): 4138-4152 DOI: 10.1016/j.jia.2024.03.027

Crop Science

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Identification and transfer of resistance to Fusarium head blight from Elymus repens chromosome arm 7StL into wheat

Fei Wang^{1, 2*}, Xin Zhao^{1, 2*}, Xianghai Yu^{1, 2}, Wei Zhu^{1, 2}, Lili Xu², Yiran Cheng¹, Yazhou Zhang^{1, 2}, Yi Wang^{1, 2}, Jian Zeng³, Xing Fan^{1, 2}, Lina Sha⁴, Haiqin Zhang⁴, Yonghong Zhou^{1, 2}, Dandan Wu^{1, 2#}, Houyang Kang^{1, 2#}

¹State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China

²Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China

³College of Resources, Sichuan Agricultural University, Chengdu 611130, China

⁴College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China

Highlights
● Development and identification of a new wheat–Elymus repens 7DS·7StL translocation line with Fusarium head blight (FHB) resistance.
● Elymus repens chromosome arm 7StL carries the dominant FHB resistance locus, preliminarily designated as QFhb.Er-7StL.
● The 7DS·7StL translocation confers a significant enhancement of FHB resistance in wheat.

Abstract
References

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摘要

小麦赤霉病（Fusarium head blight，FHB）主要是由禾谷镰孢菌（Fusarium graminearum，Fg）引起的一种严重危害小麦生产的真菌病害。偃麦草（Elymus repens, 2n=6x=42，StStStHH）是小麦的野生近缘种，具有许多抗生物和非生物胁迫的优良性状。为了将该野生种质的抗赤霉病基因转移并应用于小麦育种，我们从偃麦草与普通小麦杂交的衍生后代中鉴定了一个新的抗赤霉病易位系K140-7。通过基因组原位杂交（GISH）、非变性-荧光原位杂交（ND-FISH）、寡核苷酸-FISH涂染（Oligo-FISH painting）、单拷贝基因-FISH（single gene FISH）和小麦55K SNP芯片分析，发现K140-7是一个细胞遗传学稳定的7DS·7StL易位系，易位断点（340.8-342.5 Mb）靠近染色体7D的着丝粒区域（336.3-341.7 Mb）。基于二倍体拟鹅观草（Pseudoroegneria libanotica）St参考基因组，开发了21个7StL特异的简单序列重复（SSR）分子标记。在不同小麦背景中对7DS·7StL易位进行基因型和表型分析，表明外源片段7StL赋予了小麦赤霉病抗性且抗病性为显性，将该赤霉病抗性位点命名为QFhb.Er-7StL。进一步将该位点向三种遗传背景的小麦品种进行转移，创制了3份农艺性状更为优良的第二代7DS·7StL易位系，可用于小麦赤霉病抗性遗传改良。

Abstract

Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is one of the most destructive fungal diseases affecting global wheat production. Elymus repens (2n=6×=42, StStStStHH), a wild relative of wheat, exhibits numerous biotic and abiotic stress resistance characteristics. In previous studies, FHB resistance of E. repens has been transferred into common wheat through a wheat–E. repens partial amphidiploid and derivative lines. This study reports the development, characterization, and breeding utilization of K140-7, a novel wheat–E. repens translocation line conferring resistance to FHB. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) analyses demonstrated that K140-7 contained 40 common wheat chromosomes and two 7D·St translocation chromosomes. Subsequent characterization using oligonucleotide-FISH painting and single-gene FISH markers confirmed that the 7D fragment was a 7D short arm and the St fragment was a 7St long arm. Therefore, K140-7 was further identified as a 7DS·7StL translocation line with genetic compensation. Wheat 55K SNP array analysis of K140-7 demonstrated the 7DS·7StL translocation event. Field evaluations demonstrated that K140-7 exhibits agronomic performance comparable to its wheat parent. Based on St reference genome of Pseudoroegneria libanotica, 21 simple sequence repeats (SSR) markers specific to 7StL were developed. Genetic analysis established that 7StL confers FHB resistance and carries the dominant FHB resistance locus, designated as QFhb.Er-7StL. Introgression of QFhb.Er-7StL into elite wheat cultivars has generated three second-generation 7DS·7StL translocation lines with enhanced agronomic traits. This study provides valuable novel germplasms and specific molecular markers for FHB resistance breeding in wheat.

Keywords: Fusarium head blight (FHB) Elymus repens wheat translocation line breeding transfer

Received: 01 November 2023 Accepted: 03 January 2024 Online: 06 March 2024

Fund: This work was supported by the National Natural Science Foundation of China (31971883 and 32200180), the Major Program of National Agricultural Science and Technology of China (NK20220607), the Special Projects of the Central Government in Guidance of Local Science and Technology Development, China (2023ZYD0088), the Science and Technology Bureau of Sichuan Province, China (2021YFYZ0002, 2022ZDZX0014, 2023NSFSC1995, and 2022YFSY0035), and the Science and Technology Bureau of Chengdu City, China (2022-YF05-00449-SN).

About author: #Correspondence Houyang Kang, Tel: +86-28-86293067, E-mail: houyang.kang@sicau.edu.cn; Dandan Wu, Tel: +86-28-82650350, E-mail: wudandan@sicau.edu.cn * These authors contributed equally to this study.

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Fei Wang, Xin Zhao, Xianghai Yu, Wei Zhu, Lili Xu, Yiran Cheng, Yazhou Zhang, Yi Wang, Jian Zeng, Xing Fan, Lina Sha, Haiqin Zhang, Yonghong Zhou, Dandan Wu, Houyang Kang. 2025. Identification and transfer of resistance to Fusarium head blight from Elymus repens chromosome arm 7StL into wheat. Journal of Integrative Agriculture, 24(11): 4138-4152.

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