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| In vitro and in silico studies of salicylic acid on systemic induced resistance against bacterial leaf blight disease and enhancement of crop yield |
Wannaporn THEPBANDIT1, Narendra Kumar PAPATHOTI1, Jayasimha Rayulu DADDAM2, Nguyen Huy HOANG1, Toan LE THANH3, Chanon SAENGCHAN1, Kumrai BUENSANTEAI1
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1 School of Crop Production Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
2 Department of Animal Science, Agriculture Research Organization, Rishon Lezion 7505101, Israel
3 Department of Plant Protection, Can Tho University, Can Tho 900000, Vietnam
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Abstract
Salicylic acid (SA) is an effective elicitor to promote plant defenses and growth. This study aimed to investigate rice (Oryza sativa L.) cv. Khao Dawk Mali 105 treated with salicylic acid (SA)-Ricemate as an enhanced plant protection mechanism against bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo). Results indicated that the use of SA-Ricemate as a foliar spray at concentrations of more than 100 mg L–1 can reduce the severity of BLB by 71%. SA-Ricemate treatment also increased the hydrogen peroxide (H2O2) content of rice leaf tissues over untreated samples by 39–61%. Malondialdehyde (MDA) in rice leaves treated with SA-Ricemate also showed an increase of 50–65% when comparing to non-treated samples. The differential development of these defense compounds was faster and distinct when the SA-Ricemate-treated rice was infected with Xoo, indicating plant-induced resistance. Besides, SA-Ricemate elicitor at a concentration of 50–250 mg L–1 was correlated with a substantial increase in the accumulation of total chlorophyll content at 2.53–2.73 mg g–1 of fresh weight which suggests that plant growth is activated by SA-Ricemate. The catalase- and aldehyde dehydrogenase-binding sites were searched for using the CASTp server, and the findings were compared to the template. Chemsketch was used to design and optimize SA, which was then docked to the catalase and aldehyde dehydrogenase-binding domains of the enzymes using the GOLD 3.0.1 Software. SA is shown in several docked conformations with the enzymes catalase and aldehyde dehydrogenase. All three catalase amino acids (GLN7, VAL27, and GLU38) were discovered to be involved in the creation of a strong hydrogen bond with SA when SA was present. In this mechanism, the aldehyde dehydrogenase amino acids LYS5, HIS6, and ASP2 were all implicated, and these amino acids created strong hydrogen bonds with SA. In field conditions, SA-Ricemate significantly reduced disease severity by 78% and the total grain yield was significantly increased which was an increase of plant height, tiller per hill, and panicle in three field trials during Aug–Nov 2017 and 2018. Therefore, SA-Ricemate can be used as an alternative elicitor on replacing harmful pesticides to control BLB disease with a high potential of increasing rice defenses, growth, and yield components.
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Received: 21 December 2021
Accepted: 13 February 2022
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This work was supported by the Suranaree University of Technology, Thailand, the Thailand Science Research and Innovation (TSRI), and the National Science, Research and Innovation Fund, Thailand (NSRF) (90464).
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| About author: Wannaporn THEPBANDIT, E-mail: w.thepbandit@gmail.com; Correspondence Kumrai BUENSANTEAI, E-mail: kumrai@sut.ac.th |
Cite this article:
Wannaporn THEPBANDIT, Narendra Kumar PAPATHOTI, Jayasimha Rayulu DADDAM, Nguyen Huy HOANG, Toan LE THANH, Chanon SAENGCHAN, Kumrai BUENSANTEAI.
2023.
In vitro and in silico studies of salicylic acid on systemic induced resistance against bacterial leaf blight disease and enhancement of crop yield. Journal of Integrative Agriculture, 22(1): 170-184.
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Adss I, Hamza H, Hafez E, Heikal H. 2017. Enhancing tomato fruits post-harvest resistance by salicylic acid and hydrogen peroxide elicitors against rot caused by Alternaria solani. Mansoura Journal of Agriclture Chemistry and Biochemistry, 8, 1–8.
Alvarez M E, Pennell R I, Meijer P J, Ishikawa A, Dixon, R A, Lamb C. 1998. Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell, 92, 773–784.
Aminot A, Rey F. 2002. Standard procedure for the determination of chlorophyll a by spectroscopic methods. ICES: Techniques in Marine Environmental Sciences, 30, 1–17.
Aranega-Bou O L, de la Finiti I M, García-Agustín P, González-Bosch C. 2014. Priming of plant resistance by natural compounds. Hexanoic acid as a model. Frontiers in Plant Science, 5, 488.
Bastas K K. 2014. Importance of reactive oxygen species in plant–pathogen interactions. Selcuk Journal of Agriculture and Food Sciences, 28, 11–26.
Bolwell G P. 1999. Role of active oxygen species and NO in plant defence responses. Current Opinion in Plant Biology, 2, 287–294.
Buensanteai N, Yuen G, Prathuangwong S. 2008. The biocontrol bacterium Bacillus amyloliquefaciens KPS46 produces auxin, surfactin and extracellular proteins for enhanced growth of soybean plant. Thai Journal of Agricultural Science, 41, 101–116.
Chithrashree, Udayashankar A C, Chandra Nayaka S, Reddy M S, Srinivas C. 2011. Plant growth-promoting rhizobacteria mediate induced systemic resistance in rice against bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. Biological Control, 59, 114–122.
Corbineau F, Gay-Mathieu C, Vinel D, Côme D. 2002. Decrease in sunflower (Helianthus annuus) seed viability caused by high temperature as related to energy metabolism, membrane damage and lipid composition. Physiologia Plantarum, 116, 489–496.
Daddam J, Sreenivasulu B, Peddanna K, Umamahesh K. 2020a. Designing, docking and molecular dynamics simulation studies of novel cloperastine analogues as anti-allergic agents: Homology modeling and active site prediction for the human histamine H1 receptor. RSC Advances, 10, 4745–4754.
Daddam J, Sreenivasulu B, Umamahesh K, Peddanna K, Rao D. 2020b. In silico studies on anti-stress compounds of ethanolic root extract of Hemidesmus indicus L. Current Pharmaceutical Biotechnology, 21, 502–515.
Daddam J R, Dowlathabad M, Panthangi S, Jasti P. 2014. Molecular docking and P-glycoprotein inhibitory activity of flavonoids. Interdisciplinary Sciences, 6, 167–175.
Deenamo N, Kuyyogsuy A, Khompatara K, Chanwun T, Ekchaweng K, Churngchow N. 2018. Salicylic acid induces resistance in rubber tree against Phytophthora palmivora. International Journal of Molecular Sciences, 19, 1883.
Dempsey D A, Klessig D F. 1995. Signals in plant disease resistance. Microbes and Infection, 93, 167–186.
Dempsey D M A, Klessig D F. 2017. How does the multifaceted plant hormone salicylic acid combat disease in plants and are similar mechanisms utilized in humans? BMC Biology, 15, 23–23.
Draper H H, Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology, 186, 421–431.
Ferreira D, Falahati F, Linden C, Buckley R F, Ellis K A, Savage G, Villemagne V L, Rowe C C, Ames D, Simmons A, Westman E. 2017. A ‘Disease Severity Index’ to identify individuals with Subjective Memory Decline who will progress to mild cognitive impairment or dementia. Scientific Reports, 7, 44368.
Furniss J J, Spoel S H. 2015. Cullin-RING ubiquitin ligases in salicylic acid-mediated plant immune signaling. Frontiers in Plant Science, 6, 154.
Gao M, Yin X, Yang W, Lam S M, Tong X, Liu J, Wang X, Li Q, Shui G, He Z. 2017. GDSL lipases modulate immunity through lipid homeostasis in rice. PLoS Pathogens, 13, e1006724.
Gaweł S, Wardas M, Niedworok E, Wardas P. 2004. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci Lekarskie (Warsaw, Poland: 1960), 57, 453–455.
Ghazanfar M, Raza W, Iqbal Z, Ahmad S, Qamar M. 2019. Antifungal activity by resistance inducing salicylic acid and plant extracts against postharvest rots of tomato. International Journal of Biosciences (IJB), 14, 264–274.
Gorni P, Pacheco A. 2016. Growth promotion and elicitor activity of salicylic acid in Achillea millefolium L. African Journal of Biotechnology, 15, 657–665.
Hadi M R, Balali G. 2010. The effect of salicylic acid on the reduction of Rizoctonia solani damage in potato. American–Eurasian Journal of Agricultural & Environmental Sciences, 7, 492–496.
Hao W, Guo H, Zhang J, Hu G, Yao Y, Dong J. 2014. Hydrogen peroxide is involved in salicylic acid-elicited rosmarinic acid production in Salvia miltiorrhiza cell cultures. Scientific World Journal, 2014, 843764.
Harathi N, Reddy S, Sura M, Daddam J. 2021. Structure prediction, molecular simulations of RmlD from Mycobacterium tuberculosis, and interaction studies of Rhodanine erivatives for anti-tuberculosis activity. Journal of Molecular Modeling, 27, 75.
Hayat S, Khan N A, Alyemeni M. 2013. Salicylic Acid: Plant Growth and Development. Springer, Dordrecht, The Netherlands.
Hussain M, Hamid M I, Ghazanfar M U. 2015. Salicylic acid induced resistance in fruits to combat against postharvest pathogens: A review. Archives of Phytopathology and Plant Protection, 48, 34–42.
IRRI (International Rice Research Institute). 2017. Shaping greater research outcomes. [2017-03-20]. Retrieved from http://irri.org/our-work/research/rice-and-the-environment
Kareem F, Rihan H, Fuller M P. 2019. The effect of exogenous applications of salicylic acid on drought tolerance and up-regulation of the drought response regulon of Iraqi wheat. Journal of Crop Science and Biotechnology, 22, 37–45.
Kauss H, Jeblick W. 1995. Pretreatment of parsley suspension cultures with salicylic acid enhances spontaneous and elicited production of H2O2. Plant Physiology, 108, 1171–1178.
Kawasaki J, Herath S. 2011. Impact assessment of climate change on rice production in Khon Kaen province, Thailand. The International Society for Southeast Asian Agricultural Sciences, 17, 14–28.
Ke Y, Hui S, Yuan M. 2017. Xanthomonas oryzae pv. oryzae inoculation and growth rate on rice by leaf clipping method. Bio-Protocol, 7, e2568.
Krishnan N, Gandhi K, Peeran M F, Muthurajan R, Kuppusamy P, Thiruvengadam R. 2014. Management of bacterial leaf blight disease in rice with endophytic bacteria. World Applied Sciences Journal, 28, 2229–2241.
Kurjogi M, Satapute P, Jogaiah S, Abdelrahman M, Daddam J R, Ramu V, Tran L S P 2018. Computational modeling of the Staphylococcal enterotoxins and their interaction with natural antitoxin compounds. International Journal of Molecular Sciences, 19, 133.
Kuzniak E, Urbanek H. 2012. The involvement of hydrogen peroxide in plant responses to stresses. Acta Physiologiae Plantarum, 22, 195–203.
Leon J, Lawton M A, Raskin I. 1995. Hydrogen peroxide stimulates salicylic acid biosynthesis in tobacco. Plant Physiology, 108, 1673–1678.
López-Gresa M P, Lisón P, Yenush L, Conejero V, Rodrigo I, Bellés J M. 2016. Salicylic acid is involved in the basal resistance of tomato plants to citrus exocortis viroid and tomato spotted wilt virus. PLoS ONE, 11, e0166938.
Mousavi K S M, Khara J, Heidari R. 2009. Effect of salicylic acid on photosynthetic pigment content in ocimum bacilicum under uv-c radiation stress. Journal of Cell and Molecular Research, 1, 57–60.
Moussa H R, El-Gamal S M. 2010. Effect of salicylic acid pretreatment on cadmium toxicity in wheat. Biologia Plantarum, 54, 315–320.
Nazar R, Umar S, Khan N A, Sareer O. 2015. Salicylic acid supplementation improves
photosynthesis and growth in mustard through changes in proline accumulation and ethylene formation under drought stress. South African Journal of Botany, 98, 84–94.
Niu L, Liao W. 2016. Hydrogen peroxide signaling in plant development and abiotic responses: crosstalk with nitric oxide and calcium. Frontiers in Plant Science, 7, 230.
Noh T H, Lee D K, Park J C, Shim H K, Choi M Y, Kang M H, Kim J D. 2007. Effects of bacterial leaf blight occurrence on rice yield and grain quality in different rice growth stage. Research in Plant Disease, 13, 20–23.
Nomura H, Komori T, Uemura S, Kanda Y, Shimotani, K, Nakai K, Furuichi T, Takebayashi K, Sugimoto T, Sano S, Suwastika I N, Fukusaki E, Yoshioka H, Nakahira Y, Shiina T. 2012. Chloroplast-mediated activation of plant immune signalling in Arabidopsis. Nature Communications, 3, 926.
Pallardy S G 2008. CHAPTER 5 - Photosynthesis. In: Physiology of Woody Plants. 3 ed. Academic Press, USA.
Pancheva T V, Popova L P, Uzunova A N. 1996. Effects of salicylic acid on growth and photosynthesis in barley plants. Journal of Plant Physiology, 149, 57–63.
Papathoti N, Lingampally N, Parameshwar J, Khan M, Rayalu J, Hameeda B. 2016. In silico and in vitro studies of fungicidal nature of lipopeptide (Iturin A) from Bacillus amyloliquefaciens RHNK 22 and its plant growth promoting traits. Indian Phytopathology, 69, 569–574.
Parashar A, Yusuf M, Fariduddin Q, Ahmad A. 2014. Salicylic acid enhances antioxidant system in Brassica juncea grown under different levels of manganese. International Journal of Biological Macromolecules, 70, 551–558.
Peng H, Chen Z, Fang Z, Zhou J, Xia Z, Gao L, Chen L, Li L, Li T, Zhai W, Zhang W. 2015. Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection. Scientific Reports, 5, 12165.
Razmi N, Ebadi A, Daneshian J, Jahanbakhsh S. 2017. Salicylic acid induced changes on antioxidant capacity, pigments and grain yield of soybean genotypes in water deficit condition. Journal of Plant Interactions, 12, 457–464.
Reddy D R M, Rouse D I, Rao A V. 1979. Relationship of bacterial leaf blight caused by Xanthomonas oryzae severity to grain yield of rice. Phytopathology, 69, 967–969.
Regalado M J, Ramos P. 2018. Field Testing of a Rice Crop Postharvest Management Protocol for Reduced Postproduction Losses and Improved Product Quality. Philippine Rice Research Institute, Maligaya, Science City of Munoz, Nueva Ecija, Philippines.
Rice I N F G E O, Institute I R R. 1996. Standard Evaluation System for Rice. International Rice Research Institute, Philippines.
Sadeghipour O. 2012. Impact of exogenous salicylic acid application on some traits of common bean (Phaseolus vulgaris L.) under water stress conditions. International Journal of Agriculture and Crop Science, 4, 685–690.
Sahebani N, Hadavi N. 2009. Induction of H2O2 and related enzymes in tomato roots infected with root knot nematode (M. javanica) by several chemical and microbial elicitors. Biocontrol Science and Technology, 19, 301–313.
Saleh A, Abduljabbar I. 2019. Review on the Role of Salicylic Acid in Plants. Al-Musaib Technical College/Al-Furat Al-Awsat Technical University, Iraq.
Shaik A H, Shaik S R, Daddam J R, Ali D, Manoharadas S, Arafah M W, Kodidhela L D 2021.
Maslinic acid and gallic acid protective efficacy on lipids, lipoproteins and lipid metabolizing enzymes against isoproterenol administered cardiotoxicity: An in vivo and in silico molecular docking evidences. Journal of King Saud University (Science), 33, 101230.
Silverman F, Seskar M, Kanter D, Schweizer P, Metraux J P, Raskin I. 1995. Salicylic acid in rice (biosynthesis, conjugation, and possible role). Plant Physiology, 108, 633–639.
Singh V K, Upadhyay R S 2014. Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L. Botanical Studies, 55, 66.
Smith S M, Li C, Li J. 2017. Hormone function in plants. In: Li J, Li C, Smith S M, eds., Hormone Metabolism and Signaling in Plants. Academic Press, USA. pp. 1–38.
Sood N, Sohal B, Lore J. 2013. Foliar application of benzothiadiazole and salicylic acid to combat sheath blight disease of rice. Rice Science, 20, 349–355.
Thakur M, Sohal B S. 2013. Role of elicitors in inducing resistance in plants against pathogen infection: A review. ISRN Biochemistry, 2013, 762412.
Le Thanh T, Thumanu K, Wongkaew S, Boonkerd N, Teaumroong N, Phansak P, Buensanteai N. 2017. Salicylic acid-induced accumulation of biochemical components associated with resistance against Xanthomonas oryzae pv. oryzae in rice. Journal of Plant Interactions, 12, 108–120.
Thepbandit W, Papathoti N K, Daddam J R, Thumanu K, Siriwong S, Le Thanh T, Buensanteai N. 2021. Identification of salicylic acid mechanism against leaf blight disease in Oryza sativa by SR-FTIR microspectroscopic and docking studies. Pathogens, 10, 652.
Timsina J, Wolf J, Guilpart N, van Bussel L G J, Grassini P, van Wart J, Hossain A, Rashid H, Islam S, van Ittersum M K. 2018. Can Bangladesh produce enough cereals to meet future demand? Agricultural Systems, 163, 36–44.
Tripathi D, Raikhy G, Kumar D. 2019. Chemical elicitors of systemic acquired resistance - Salicylic acid and its functional analogs. Current Plant Biology, 17, 48–59.
Ueda H, Kugimiya S, Tabata J, Kitamoto H, Mitsuhara I. 2019. Accumulation of salicylic acid in tomato plant under biological stress affects oviposition preference of Bemisia tabaci. Journal of Plant Interactions, 14, 73–78.
Walters D R, Ratsep J, Havis N D. 2013. Controlling crop diseases using induced resistance: challenges for the future. Journal of Experimental Botany, 64, 1263–1280.
War A R, Paulraj M G, War M Y, Ignacimuthu S. 2011. Role of salicylic acid in induction of plant defense system in chickpea (Cicer arietinum L.). Plant Signaling and Behavior, 6, 1787–1792.
Zhang Y, Smith P, Maximova S N, Guiltinan M J. 2015. Application of glycerol as a foliar spray activates the defence response and enhances disease resistance of Theobroma cacao. Molecular Plant Pathology, 16, 27–37.
Zoeller M, Stingl N, Krischke M, Fekete A, Waller F, Berger S, Mueller M J. 2012. Lipid profiling of the Arabidopsis hypersensitive response reveals specific lipid peroxidation and fragmentation processes: Biogenesis of pimelic and azelaic acid. Plant Physiology, 160, 365–378.
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