Please wait a minute...
Journal of Integrative Agriculture  2022, Vol. 21 Issue (7): 2065-2075    DOI: 10.1016/S2095-3119(21)63805-4
Special Issue: 动物科学合辑Animal Science
Animal Science · Veterinary Medicine Advanced Online Publication | Current Issue | Archive | Adv Search |
Integration of genome-wide association study and selection signatures reveals genetic determinants for skeletal muscle production traits in an F2 chicken population
LI Yu-dong1, 2, 3, BAI Xue1, 2, 3, LIU Xin1, 2, 3, WANG Wei-jia1, 2, 3, LI Zi-wei1, 2, 3, WANG Ning1, 2, 3, XIAO Fan4, GAO Hai-he4, GUO Huai-shun4, LI Hui1, 2, 3, WANG Shou-zhi1, 2, 3
1 Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150030, P.R.China 2 Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, P.R.China 3 School of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, P.R.China 4 Fujian Sunnzer Biotechnology Development Co., Ltd., Guangze 354100, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

本研究以东北农业大学鸡F2资源群体(NEAURP)为材料,利用 Illumina HiSeq PE150平台进行全基因组测序(26个F0个体进行10×重测序,519个F2个体进行3×重测序)。使用SAMtools进行SNP calling,BEAGLE 4.0在默认参数设置下进行基因型填充。经过质量控制和基因型填充后,共有7,890,258个SNPs用于分析。根据GRCg6a参考基因组,使用ANNOVAR软件进行SNP注释。基于混合线性模型(MLM),使用GEMMA软件进行全基因组关联分析。使用FST和π两种选择信号方法评估F2群体的遗传分化和遗传多样性。 GWAS与选择信号的整合分析表明,控制鸡骨骼肌产肉性状的遗传因子主要位于第1染色体(168.95Mb-172.43Mb)和第4染色体(74.37Mb-75.23Mb)上,共鉴定出17个可能影响目标性状的位置候选基因( LRCH1、CDADC1、CAB39L、LOC112531568、LOC112531569、FAM124A、FOXO1、NBEA、GPALPP1、RUBCNL、ARL11、KPNA3、LHFP、GBA3、LOC112532426、KCNIP4、SLIT2),其中KPNA3FOXO1是与鸡产肉性状相关的强烈候选基因。本研究的主要创新点是结合GWAS和选择信号分析方法解析鸡骨骼肌产肉性状的遗传结构,发现了一些新的影响鸡产肉性状的基因组区域和候选基因。



Abstract  Improving the production of broiler chicken meat has been a goal of broiler breeding programs worldwide for many years.  However, the genetic architectures of skeletal muscle production traits in chickens have not yet been fully elucidated.  In the present study, a total of 519 F2 birds, derived from a cross of Arbor Acres broiler and Baier layer, were re-sequenced (26 F0 individuals were re-sequenced at a 10-fold depth; 519 F2 individuals were re-sequenced at a 3-fold depth) and the coupling of genome-wide association study (GWAS) and selection signatures (FST (fixation index) and θπ (nucleotide diversity)) was carried out to pinpoint the associated loci and genes that contribute to pectoral muscle weight (PMW) and thigh muscle weight (TMW).  A total of 7 890 258 single nucleotide polymorphisms (SNPs) remained to be analyzed after quality control and imputation.  The integration of GWAS and selection signature analyses revealed that genetic determinants responsible for skeletal muscle production traits were mainly localized on chromosomes 1 (168.95–172.43 Mb) and 4 (74.37–75.23 Mb).  A total of 17 positional candidate genes (PCGs) (LRCH1, CDADC1, CAB39L, LOC112531568, LOC112531569, FAM124A, FOXO1, NBEA, GPALPP1, RUBCNL, ARL11, KPNA3, LHFP, GBA3, LOC112532426, KCNIP4, and SLIT2) were identified in these regions.  In particular, KPNA3 and FOXO1 were the most promising candidates for meat production in chickens.  These findings will help enhance our understanding of the genetic architecture of chicken muscle production traits, and the significant SNPs identified could be promising candidates for integration into practical breeding programs such as genome-wide selection (GS) to improve the meat yield of chickens.


Keywords:  chicken       muscle growth and development       GWAS       selection signature  
Received: 06 June 2021   Accepted: 20 July 2021
Fund: This work was supported by the National Natural Science Foundation of China (31572394), the China Agriculture Research System of MOF and MARA (CARS-41), and the White Feather Broiler Breeding Joint Project of the Ministry of Agriculture and Rural Affairs of China (19190526).
About author:  LI Yu-dong, E-mail: 710827104@qq.com, liyudong886@163.com; Correspondence WANG Shou-zhi, Tel: +86-451-55191495, E-mail: shouzhiwang@neau.edu.cn

Cite this article: 

LI Yu-dong, BAI Xue, LIU Xin , WANG Wei-jia, LI Zi-wei, WANG Ning, XIAO Fan, GAO Hai-he, GUO Huai-shun, LI Hui, WANG Shou-zhi. 2022. Integration of genome-wide association study and selection signatures reveals genetic determinants for skeletal muscle production traits in an F2 chicken population. Journal of Integrative Agriculture, 21(7): 2065-2075.

Abdalhag M A, Zhang T, Fan Q C, Zhang X Q, Zhang G X, Wang J Y, Wei Y, Wang Y J. 2015. Single nucleotide polymorphisms associated with growth traits in Jinghai yellow chickens. Genetics and Molecular Research (GMR), 14, 16169–16177.
Andersson L, Georges M. 2004. Domestic-animal genomics: Deciphering the genetics of complex traits. Nature Reviews Genetics, 5, 202–212.
Ankra-Badu G A, Shriner D, Le Bihan-Duval E, Mignon-Grasteau S, Pitel F, Beaumont C, Duclos M J, Simon J, Porter T E, Vignal A, Cogburn L A, Allison D B, Yi N, Aggrey S E. 2010. Mapping main, epistatic and sex-specific QTL for body composition in a chicken population divergently selected for low or high growth rate. BMC Genomics, 11, 107.
Baldi G, Soglia F, Laghi L, Tappi S, Rocculi P, Tavaniello S, Prioriello D, Mucci R, Maiorano G, Petracci M. 2019. Comparison of quality traits among breast meat affected by current muscle abnormalities. Food Research International, 115, 369–376.
Browning B L, Browning S R. 2009. A unified approach to genotype imputation and haplotype-phase inference for large data sets of trios and unrelated individuals. American Journal of Human Genetics, 84, 210–223.
Chen B, Xu J, He X, Xu H, Li G, Du H, Nie Q, Zhang X. 2015. A genome-wide mRNA screen and functional analysis reveal FOXO3 as a candidate gene for chicken growth. PLoS ONE, 10, e0137087.
Dou T, Li Z, Wang K, Liu L, Rong H, Xu Z, Huang Y, Gu D, Chen X, Hu W, Zhang J, Zhao S, Jois M, Li Q, Ge C, Te Pas M F W, Jia J. 2018. Regulation of myostatin expression is associated with growth and muscle development in commercial broiler and DMC muscle. Molecular Biology Reports, 45, 511–522.
Duggal P, Gillanders E M, Holmes T N, Bailey-Wilson J E. 2008. Establishing an adjusted p-value threshold to control the family-wide type 1 error in genome wide association studies. BMC Genomics, 9, 516.
Godoy T F, Moreira G C, Boschiero C, Gheyas A A, Gasparin G, Paduan M, Andrade S C, Montenegro H, Burt D W, Ledur M C, Coutinho L L. 2015. SNP and INDEL detection in a QTL region on chicken chromosome 2 associated with muscle deposition. Animal Genetics, 46, 158–163.
Grossman S R, Shlyakhter I, Karlsson E K, Byrne E H, Morales S, Frieden G, Hostetter E, Angelino E, Garber M, Zuk O, Lander E S, Schaffner S F, Sabeti P C. 2010. A composite of multiple signals distinguishes causal variants in regions of positive selection. Science, 327, 883–886.
Hirschhorn J N, Daly M J. 2005. Genome-wide association studies for common diseases and complex traits. Nature Reviews Genetics, 6, 95–108.
Hu Z L, Park C A, Reecy J M. 2019. Building a livestock genetic and genomic information knowledgebase through integrative developments of Animal QTLdb and CorrDB. Nucleic Acids Research, 47, D701–D710.
Huang Y, Xiao L, Zhang Z, Zhang R, Wang Z, Huang C, Huang R, Luan Y, Fan T, Wang J, Shen C, Zhang S, Wang X, Randall J, Zheng B, Wu J, Zhang Q, Xia G, Xu C, Chen M, et al. 2019. The genomes of pecan and Chinese hickory provide insights into Carya evolution and nut nutrition. Gigascience, 8, giz036. 
Jia X, Ouyang H, Abdalla B A, Xu H, Nie Q, Zhang X. 2017. MiR-16 controls myoblast proliferation and apoptosis through directly suppressing BCL2 and FOXO1 activities. Biochimica et Biophysica Acta Gene Regulatory Mechanisms, 1860, 674–684.
Kamei Y, Miura S, Suzuki M, Kai Y, Mizukami J, Taniguchi T, Mochida K, Hata T, Matsuda J, Aburatani H, Nishino I, Ezaki O. 2004. Skeletal muscle FOXO1 (FKHR) transgenic mice have less skeletal muscle mass, down-regulated Type I (slow twitch/red muscle) fiber genes, and impaired glycemic control. The Journal of Biological Chemistry, 279, 41114–41123. 
Kong B W, Hudson N, Seo D, Lee S, Khatri B, Lassiter K, Cook D, Piekarski A, Dridi S, Anthony N, Bottje W. 2017. RNA sequencing for global gene expression associated with muscle growth in a single male modern broiler line compared to a foundational Barred Plymouth Rock chicken line. BMC Genomics, 18, 82. 
Lee S, Dong H H. 2017. FoxO integration of insulin signaling with glucose and lipid metabolism. The Journal of Endocrinology, 233, R67–R79. 
Leng L, Wang S, Li Z, Wang Q, Li H. 2009. A polymorphism in the 3´-flanking region of insulin-like growth factor binding protein 2 gene associated with abdominal fat in chickens. Poultry Science, 88, 938–942.
Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R, 1000 Genome Project Data Processing Subgroup. 2009. The sequence alignment/map format and SAMtools. Bioinformatics, 25, 2078–2079.
Li M, Tian S, Jin L, Zhou G, Li Y, Zhang Y, Wang T, Yeung C K, Chen L, Ma J, Zhang J, Jiang A, Li J, Zhou C, Zhang J, Liu Y, Sun X, Zhao H, Niu Z, Lou P, et al. 2013. Genomic analyses identify distinct patterns of selection in domesticated pigs and Tibetan wild boars. Nature Genetics, 45, 1431–1438.
Liu L X, Dou T F, Li Q H, Rong H, Tong H Q, Xu Z Q, Huang Y, Gu D H, Chen X B, Ge C R, Jia J J. 2016. Myostatin mRNA expression and its association with body weight and carcass traits in Yunnan Wuding chicken. Genetics and Molecular Research, 15, doi: 10.4238/gmr15048967.
Liu X, Zhang H, Li H, Li N, Zhang Y, Zhang Q, Wang S, Wang Q, Wang H. 2008. Fine-mapping quantitative trait loci for body weight and abdominal fat traits: Effects of marker density and sample size. Poultry Science, 87, 1314–1319.
Liu Y, Yang X, Jing X, He X, Wang L, Liu Y, Liu D. 2017. Transcriptomics analysis on excellent meat quality traits of skeletal muscles of the chinese indigenous Min Pig compared with the Large White breed. International Journal of Molecular Sciences, 19, 21. 
Liu Z, Sun C J, Qu L, Wang K H, Yang N. 2016. Genome-wide detection of selective signatures in chicken through high density SNPs. PLoS ONE, 11, e0166146.
Lyu S, Arends D, Nassar M K, Brockmann G A. 2017. Fine mapping of a distal chromosome 4 QTL affecting growth and muscle mass in a chicken advanced intercross line. Animal Genetics, 48, 295–302.
Mariadassou M, Ramayo-Caldas Y, Charles M, Femenia M, Renand G, Rocha D. 2020. Detection of selection signatures in Limousin cattle using whole-genome resequencing. Animal Genetics, 51, 815–819.
McCarthy M I, Abecasis G R, Cardon L R, Goldstein D B, Little J, Ioannidis J P, Hirschhorn J N. 2008. Genome-wide association studies for complex traits: Consensus, uncertainty and challenges. Nature Reviews Genetics, 9, 356–369.
Meyer K. 2007. WOMBAT: A tool for mixed model analyses in quantitative genetics by restricted maximum likelihood (REML). Journal of Zhejiang University Science (B), 8, 815–821. 
Le Mignon G, Pitel F, Gilbert H, Le Bihan-Duval E, Vignoles F, Demeure O, Lagarrigue S, Simon J, Cogburn L A, Aggrey S E, Douaire M, Le Roy P. 2009. A comprehensive analysis of QTL for abdominal fat and breast muscle weights on chicken chromosome 5 using a multivariate approach. Animal Genetics, 40, 157–164.
Mir N A, Rafiq A, Kumar F, Singh V, Shukla V. 2017. Determinants of broiler chicken meat quality and factors affecting them: A review. Journal of Food Science and Technology, 54, 2997–3009.
NRC (National Research Council). 1994. Nutrient Requirements of Poultry. 9th ed. National Academies Press, Washington, D.C.
Olszewski P K, Rozman J, Jacobsson J A, Rathkolb B, Strömberg S, Hans W, Klockars A, Alsiö J, Risérus U, Becker L, Hölter S M, Elvert R, Ehrhardt N, Gailus-Durner V, Fuchs H, Fredriksson R, Wolf E, Klopstock T, Wurst W, Levine A S, et al. 2012. Neurobeachin, a regulator of synaptic protein targeting, is associated with body fat mass and feeding behavior in mice and body-mass index in humans. PLoS Genetics, 8, e1002568.
O’Neill B T, Lee K Y, Klaus K, Softic S, Krumpoch M T, Fentz J, Stanford K I, Robinson M M, Cai W, Kleinridders A, Pereira R O, Hirshman M F, Abel E D, Accili D, Goodyear L J, Nair K S, Kahn C R. 2016. Insulin and IGF-1 receptors regulate FoxO-mediated signaling in muscle proteostasis. The Journal of Clinical Investigation, 126, 3433–3446. 
Pampouille E, Berri C, Boitard S, Hennequet-Antier C, Beauclercq S A, Godet E, Praud C, Jégo Y, Le Bihan-Duval E. 2018. Mapping QTL for white striping in relation to breast muscle yield and meat quality traits in broiler chickens. BMC Genomics, 19, 202.
Pertille F, Zanella R, Felicio A M, Ledur M C, Peixoto J O, Coutinho L L. 2015. Identification of polymorphisms associated with production traits on chicken (Gallus gallus) chromosome 4. Genetics and Molecular Research, 14, 10717–10728.
Petracci M, Cavani C. 2012. Muscle growth and poultry meat quality issues. Nutrients, 4, 1–12.
Sato S, Ohtake T, Uemoto Y, Okumura Y, Kobayashi E. 2012. Polymorphism of insulin-like growth factor 1 gene is associated with breast muscle yields in chickens. Animal Science Journal, 83, 1–6.
Wang K, Li M Y, Hakonarson H. 2010. ANNOVAR: Functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Research, 38, e164.
Wright D, Rubin C J, Martinez Barrio A, Schütz K, Kerje S, Brändström H, Kindmark A, Jensen P, Andersson L. 2010. The genetic architecture of domestication in the chicken: Effects of pleiotropy and linkage. Molecular Ecology, 19, 5140–5156.
Xie L, Luo C, Zhang C, Zhang R, Tang J, Nie Q, Ma L, Hu X, Li N, Da Y, Zhang X. 2012. Genome-wide association study identified a narrow chromosome 1 region associated with chicken growth traits. PLoS ONE, 7, e30910.
Xu L, Yang L, Wang L, Zhu B, Chen Y, Gao H, Gao X, Zhang L, Liu G E, Li J. 2019. Probe-based association analysis identifies several deletions associated with average daily gain in beef cattle. BMC Genomics, 20, 31.
Yu Y, Fuscoe J C, Zhao C, Guo C, Jia M, Qing T, Bannon D I, Lancashire L, Bao W, Du T, Luo H, Su Z, Jones W D, Moland C L, Branham W S, Qian F, Ning B, Li Y, Hong H, Guo L, et al. 2014. A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages. Nature Communications, 5, 3230.
Zhang G X, Zhang T, Wei Y, Ding F X, Zhang L, Wang J Y. 2015. Functional identification of an exon 1 substitution in the myostatin gene and its expression in breast and leg muscle of the Bian chicken. British Poultry Science, 56, 639–644.
Zhang H, Liang Q, Wang N, Wang Q, Leng L, Mao J, Wang Y, Wang S, Zhang J, Liang H, Zhou X, Li Y, Cao Z, Luan P, Wang Z, Yuan H, Wang Z, Zhou X, Lamont S J, Da Y, et al. 2020. Microevolutionary dynamics of chicken genomes under divergent selection for adiposity. Science, 23, 101193. 
Zhang H, Liu S H, Zhang Q, Zhang Y D, Wang S Z, Wang Q G, Wang Y X, Tang Z Q, Li H. 2011. Fine-mapping of quantitative trait loci for body weight and bone traits and positional cloning of the RB1 gene in chicken. Journal of Animal Breeding and Genetics, 128, 366–375.
Zhang H, Zhang Y D, Wang S Z, Liu X F, Zhang Q, Tang Z Q, Li H. 2010. Detection and fine mapping of quantitative trait loci for bone traits on chicken chromosome one. Journal of Animal Breeding and Genetics, 127, 462–468.
Zhang Z, Chen Z, Ye S, He Y, Huang S, Yuan X, Chen Z, Zhang H, Li J. 2019. Genome-wide association study for reproductive traits in a duroc pig population. Animals (Basel), 9, 732.
Zhou X, Stephens M. 2012. Genome-wide efficient mixed-model analysis for association studies. Nature Genetics, 44, 821–824.
Zhou Z, Li M, Cheng H, Fan W, Yuan Z, Gao Q, Xu Y, Guo Z, Zhang Y, Hu J, Liu H, Liu D, Chen W, Zheng Z, Jiang Y, Wen Z, Liu Y, Chen H, et al. 2018. An intercross population study reveals genes associated with body size and plumage color in ducks. Nature Communications, 9, 2648.

[1] Myeong-Hyeon Min, Aye Aye Khaing, Sang-Ho Chu, Bhagwat Nawade, Yong-Jin Park. Exploring the genetic basis of pre-harvest sprouting in rice through a genome-wide association study-based haplotype analysis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2525-2540.
[2] Lei Shi, Yanyan Sun, Yunlei Li, Hao Bai, Jingwei Yuan, Hui Ma, Yuanmei Wang, Panlin Wang, Aixin Ni, Linlin Jiang, Pingzhuang Ge, Shixiong Bian, Yunhe Zong, Jinmeng Zhao, Adamu M. Isa, Hailai H. Tesfay, Jilan Chen. Asymmetric expression of CA2 and CA13 linked to calcification in the bilateral mandibular condyles cause crossed beaks in chickens[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2379-2390.
[3] Yingzhen Wang, Ying Wu, Xinlei Wang, Wangmei Ren, Qinyao Chen, Sijia Zhang, Feng Zhang, Yunzhi Lin, Junyang Yue, Yongsheng Liu.

Genome wide association analysis identifies candidate genes for fruit quality and yield in Actinidia eriantha  [J]. >Journal of Integrative Agriculture, 2024, 23(6): 1929-1939.

[4] Yongchao Hao, Fanmei Kong, Lili Wang, Yu Zhao, Mengyao Li, Naixiu Che, Shuang Li, Min Wang, Ming Hao, Xiaocun Zhang, Yan Zhao.

Genome-wide association study of grain micronutrient concentrations in bread wheat [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1468-1480.

[5] Ying Ding, Qiong Zhi, Qisheng Zuo, Kai Jin, Wei Han, Bichun Li.

Transcriptome-based analysis of key signaling pathways affecting the formation of primordial germ cell in chickens [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1644-1657.

[6] Lingzhai Meng, Mengmeng Yu, Suyan Wang, Yuntong Chen, Yuanling Bao, Peng Liu, Xiaoyan Feng, Tana He, Ru Guo, Tao Zhang, Mingxue Hu, Changjun Liu, Xiaole Qi, Kai Li, Li Gao, Yanping Zhang, Hongyu Cui, Yulong Gao.

A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1658-1670.

[7] Ping Xu, Hao Li, Haiyuan Li, Ge Zhao, Shengjie Dai, Xiaoyu Cui, Zhenning Liu, Lei Shi, Xiaohua Wang.

Genome-wide and candidate gene association studies identify BnPAP17 as conferring the utilization of organic phosphorus in oilseed rape [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1134-1149.

[8] Mu Zeng, Binhu Wang, Lei Liu, Yalan Yang, Zhonglin Tang. Genome-wide association study identifies 12 new genetic loci associated with growth traits in pigs[J]. >Journal of Integrative Agriculture, 2024, 23(1): 217-227.
[9] ZHANG Jin, WANG Jie, WANG Qiao, CUI Huan-xian, DING Ji-qiang, WANG Zi-xuan, Mamadou Thiam, LI Qing-he, ZHAO Gui-ping. Immunogenetic basis of chicken’s heterophil to lymphocyte ratio revealed by genome-wide indel variants analysis[J]. >Journal of Integrative Agriculture, 2023, 22(9): 2810-2823.
[10] YAN Sheng-nan, YU Zhao-yu, GAO Wei, WANG Xu-yang, CAO Jia-jia, LU Jie, MA Chuan-xi, CHANG Cheng, ZHANG Hai-ping. Dissecting the genetic basis of grain color and pre-harvest sprouting resistance in common wheat by association analysis[J]. >Journal of Integrative Agriculture, 2023, 22(9): 2617-2631.
[11] WANG Jie, LEI Qiu-xia, CAO Ding-guo, ZHOU Yan, HAN Hai-xia, LIU Wei, LI Da-peng, LI Fu-wei, LIU Jie. Whole genome SNPs among 8 chicken breeds enable identification of genetic signatures that underlie breed features[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2200-2212.
[12] ZHAO Ruo-nan, CHEN Si-yuan, TONG Cui-hong, HAO Jie, LI Pei-si, XIE Long-fei, XIAO Dan-yu, ZENG Zhen-ling, XIONG Wen-guang. Insights into the effects of pulsed antimicrobials on the chicken resistome and microbiota from fecal metagenomes[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1857-1869.
[13] WANG Yong-li, HUANG Chao, YU Yang, CAI Ri-chun, SU Yong-chun, CHEN Zhi-wu, ZHENG Maiqing, CUI Huan-xian.

Dietary aflatoxin B1 induces abnormal deposition of melanin in the corium layer of the chicken shank possibly via promoting the expression of melanin synthesis-related genes [J]. >Journal of Integrative Agriculture, 2023, 22(6): 1847-1856.

[14] SHAN Yan-ju, JI Gai-ge, ZHANG Ming, LIU Yi-fan, TU Yun-jie, JU Xiao-jun, SHU Jing-ting, ZOU Jian-min. Use of transcriptome sequencing to explore the effect of CSRP3 on chicken myoblasts[J]. >Journal of Integrative Agriculture, 2023, 22(4): 1159-1171.
[15] CUI Huan-xian, LUO Na, GUO Li-ping, LIU Lu, XING Si-yuan, ZHAO Gui-ping, WEN Jie. TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken[J]. >Journal of Integrative Agriculture, 2023, 22(3): 853-863.
No Suggested Reading articles found!