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Journal of Integrative Agriculture  2021, Vol. 20 Issue (1): 100-108    DOI: 10.1016/S2095-3119(20)63190-2
Special Issue: 玉米耕作栽培合辑Maize Physiology · Biochemistry · Cultivation · Tillage 棉花合辑Cotton
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Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit
CHEN Yuan1, LIU Zhen-yu1, Leila I. M. Tambel2, ZHANG Xiang1, CHEN Yuan1, CHEN De-hua1 
Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College, Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China
2 Agricultural Research Cooperation, Biotechnology and Biosafety Research Center, Khartoum 999129, Sudan
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为了阐明减氮施肥对抗虫棉棉蕾中Bt杀虫蛋白含量的影响,在2015-2016棉花生长季,以常规种泗抗1号(SK-1)和杂交种泗抗3号(SK-3)为试验材料,分别进行了0、75、150、225、300 kg/公顷的施氮量处理。在不同施氮量处理下,随着施氮量从常规施氮量(300kg/公顷)降低到0 kg/公顷,单株蕾数、棉蕾体积和棉蕾干重均呈下降趋势,同时棉蕾Bt杀虫蛋白含量也相应下降。氮代谢分析结果表明,减氮条件下可溶性蛋白含量、GPT和GOT活性降低,游离氨基酸含量、肽酶和蛋白酶活性升高。相关性分析结果表明,缺氮条件下Bt蛋白含量的降低与氮代谢生理变化相关。综上所述,减氮条件下,棉蕾生长和棉蕾中Bt杀虫蛋白含量均呈下降趋势,因此在蕾期适当的施氮量在促进棉蕾生长的同时也可提高棉花抗虫性。在人间游历 爱是最壮观的迁徙。

To clarify the effect of the N deficit on the amount of square Bt insecticidal protein, different N application rates (0, 75, 150, 225, and 300 kg ha–1) were imposed on the conventional cultivar Sikang 1 (SK-1) and hybrid cultivar Sikang 3 (SK-3) during 2015–2016 cotton growth seasons.  Under different N application rates, the square number per plant, square volume and square dry weight reduced when the N rates decreased from conventional rate (300 kg ha–1) to 0 kg ha–1.  And the square Bt protein content decreased accordingly.  The analysis of N metabolism showed that soluble protein content, GPT and GOT activities decreased, free amino acid, peptidase and protease activities increased under N deficit.  Correlation analysis indicated that the reduced Bt protein content under N deficit was related to altered N metabolism.  In conclusion, square development and the amount of square Bt toxin both decreased under N deficit, indicating that promoting the square development under appropriate N application rate would also promote the insect resistance during squaring stage.
Keywords:  Bt cotton        reproductive organ        insecticidal protein       N deficit        N metabolism   
Received: 12 November 2019   Accepted:
Fund: This study was supported by the National Natural Science Foundation of China (31901462 and 31671613), the National Key Research and Development Program of China (2018YFD0100406 and 2018YFD1000907), the Natural Science Foundation of the Jiangsu Higher Education Institutions, China (18KJB210013 and 17KJA210003), and the Natural Science Foundation of Jiangsu Province, China (BK20191439).
Corresponding Authors:  Correspondence CHEN De-hua, Tel: +86-514-87979357, E-mail:   

Cite this article: 

CHEN Yuan, LIU Zhen-yu, Leila I. M. TAMBEL, ZHANG Xiang, CHEN Yuan, CHEN De-hua . 2021. Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit. Journal of Integrative Agriculture, 20(1): 100-108.

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