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| Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate |
| ZHANG Xiang, LÜ Chun-hua, CHEN Yuan, WANG Gui-xia, CHEN Yuan , CHEN De-hua |
1.Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China
2.Department of Soil and Crop Sciences, Texas A&M University, College Station 77843, USA |
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摘要 Expression of insecticidal protein for transgenic Bacillus thuringiensis (Bt) cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio (C/N) and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha-1, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of insectiocidal efficacy in Bt cotton, especially in peak boll period.
Abstract Expression of insecticidal protein for transgenic Bacillus thuringiensis (Bt) cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio (C/N) and insecticidal efficacy of two Bt cotton cultivars. C/N ratio and Bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the Bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf Bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and Bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha-1, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf Bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of insectiocidal efficacy in Bt cotton, especially in peak boll period.
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Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (31171479), the Key Laboratory Foundation of Jiangsu Province, China (10KJA210057), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China, the Doctoral Advisor Foundation of Education Department of China (20113250110001), the Project of National Agricultural Modern Industrial Technology System Post Experts, China (CARS-18-20), and the Graduate Student Scientific Research Innovation Projects of Jiangsu Province, China (CXLX11_1019). |
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Corresponding Authors:
CHEN De-hua, Tel: +86-514-87979357, Fax: +86-514-87996817, E-mail: dehuachen2002@aliyun.com
E-mail: dehuachen2002@aliyun.com
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| About author: CHEN De-hua, Tel: +86-514-87979357, Fax: +86-514-87996817, E-mail: dehuachen2002@aliyun.com |
Cite this article:
ZHANG Xiang, Lü Chun-hua, CHEN Yuan, WANG Gui-xia, CHEN Yuan , CHEN De-hua.
2014.
Relationship Between Leaf C/N Ratio and Insecticidal Protein Expression in Bt Cotton as Affected by High Temperature and N Rate. Journal of Integrative Agriculture, 13(1): 82-88.
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