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| The effects of high temperature level on square Bt protein concentration of Bt cotton |
| WANG Jun, CHEN Yuan, YAO Meng-hao, LI Yuan, WEN Yu-jin, CHEN Yuan, ZHANG Xiang, CHEN De-hua |
1、Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China
2、Department of Soil and Crop Sciences, Texas A&M University, College Station, TX77843, USA
3、Syngenta Biotechnology Inc., Carry, NC 27519, USA |
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摘要 Higher boll worm survival rates were detected after high temperature presented during square period in Bt cotton. The objective of this study was to investigate the effects of high temperature level on the Bt efficacy of two different types of Bt cotton cultivars at squaring stage. During the 2011 to 2013 cotton growth seasons, high temperature treatments ranged from 34 to 44°C in climate chambers, and field experiments under high temperature weather with various temperature levels were conducted to investigate the effects of the high temperature level on square Bt protein concentration and nitrogen metabolism. The climate chamber experiments showed that the square insecticidal protein contents reduced after 24 h elevated temperature treatments for both cultivars, whereas significant declines of the square insecticidal protein contents were detected at temperature >38°C, and only slightly numerical reductions were observed when temperature below 38°C. Similar high temperature responses were also observed at the two field experimental sites in 2013. Correspondingly, high temperature below 38°C seems have little effect on the square amino acid concentrations, soluble protein contents, glutamic- pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) activities as well as protease and peptidase activities; however, when the temperature was above 38°C, reduced soluble protein contents, enhanced amino acid concentrations, decreased GPT and GOT activities, bolstered protease and peptidase activities in square were detected. In general, the higher the temperature is (>38°C), the larger the changes for the above compound contents and key enzymes activities of the square protein cycle. The findings indicated that the unstable insect resistance of the square was related to high temperature level during square stage.
Abstract Higher boll worm survival rates were detected after high temperature presented during square period in Bt cotton. The objective of this study was to investigate the effects of high temperature level on the Bt efficacy of two different types of Bt cotton cultivars at squaring stage. During the 2011 to 2013 cotton growth seasons, high temperature treatments ranged from 34 to 44°C in climate chambers, and field experiments under high temperature weather with various temperature levels were conducted to investigate the effects of the high temperature level on square Bt protein concentration and nitrogen metabolism. The climate chamber experiments showed that the square insecticidal protein contents reduced after 24 h elevated temperature treatments for both cultivars, whereas significant declines of the square insecticidal protein contents were detected at temperature >38°C, and only slightly numerical reductions were observed when temperature below 38°C. Similar high temperature responses were also observed at the two field experimental sites in 2013. Correspondingly, high temperature below 38°C seems have little effect on the square amino acid concentrations, soluble protein contents, glutamic- pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) activities as well as protease and peptidase activities; however, when the temperature was above 38°C, reduced soluble protein contents, enhanced amino acid concentrations, decreased GPT and GOT activities, bolstered protease and peptidase activities in square were detected. In general, the higher the temperature is (>38°C), the larger the changes for the above compound contents and key enzymes activities of the square protein cycle. The findings indicated that the unstable insect resistance of the square was related to high temperature level during square stage.
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Received: 26 January 2015
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31171479, 31301263 and 31471435), the Doctoral Advisor Foundation of Ministry of Education of China (20113250110001), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD), and the Three New Technology Foundation of Agriculture in Jiangsu Province, China (SXGC(2014)317). |
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Corresponding Authors:
CHEN De-hua, Tel: +86-514-87979357,Fax: +86-514-87996817, E-mail: cdh@yzu.edu.cn
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| About author: WANG Jun, E-mail: 505417379@qq.com; |
Cite this article:
WANG Jun, CHEN Yuan, YAO Meng-hao, LI Yuan, WEN Yu-jin, CHEN Yuan, ZHANG Xiang, CHEN De-hua.
2015.
The effects of high temperature level on square Bt protein concentration of Bt cotton. Journal of Integrative Agriculture, 14(10): 1971-1979.
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