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| Effective remediation of aged HMW-PAHs polluted agricultural soil by the combination of Fusarium sp. and smooth bromegrass (Bromus inermis Leyss.) |
| SHI Wei1, ZHANG Xue-na1, JIA Hai-bin1, FENG Sheng-dong1, YANG Zhi-xin1, ZHAO Ou-ya1, LI Yu-ling2 |
1 Key Laboratory for Farmland Eco-Environment of Hebei Province/College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding 0710001, P.R.China
2 College of Forestry, Agricultural University of Hebei, Baoding 0710001, P.R.China |
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Abstract Fusarium sp. strain ZH-H2 is capable to degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs), smooth bromegrass (Bromus inermis Leyss.) can also degrade 4- to 6-ring PAHs. Pot experiments were conducted to investigate how bromegrass and different inoculum sizes of ZH-H2 clean up HMW-PAHs in agricultural soil derived from a coal mine area. The results showed that, compared with control, different sizes of inocula of ZH-H2 effectively degraded HMW-PAHs, with removal rates of 19.01, 34.25 and 29.26% for 4-, 5- and 6-ring PAHs in the treatment with 1.0 g kg–1 ZH-H2 incubation after 90 d. After 5 mon of cultivation, bromegrass reached degradation rate of these compounds by 12.66, 36.26 and 36.24%, respectively. By adding strain ZH-H2 to bromegrass, HMW-PAHs degradation was further improved up to 4.24 times greater than bromegrass (W), in addition to the degradation rate of Bbf decrease. For removal rates of both 5- and 6-ring PAHs, addition of 0.5 g kg–1 Fusarium ZH-H2 to pots with bromegrass performed better than addition of 0.1 g kg–1, while the highest concentration of 1.0 g kg–1 Fusarium ZH-H2 did not further improve degradation. Degradation of 4-ring PAHs showed no significant difference among different ZH-H2 incubations with bromegrass treatments. We found that the degradation rates of 4-, 5- and 6-ring PAHs in all treatments are significantly correlated in a positive, linear manner with activity of lignin peroxidase (LiP) (r=0.8065, 0.9350 and 0.9165, respectively), while degradation of 5- and 6-ring PAHs is correlated to polyphenoloxidase (PPO) activity (r=0.7577 and 07806). Our findings suggest that the combination of Fusarium sp. ZH-H2 and bromegrass offers a suitable alternative for phytoremediation of aged PAH-contaminated soil in coal mining areas, with a recommended inoculation size of 0.5 g Fusarium sp. ZH-H2 per kg soil.
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Received: 21 November 2015
Accepted:
Online: 19 April 2016
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| Fund: This study was supported by the National High-Tech R&D Program of China (863 Program) (2012AA101403), the Educational Commission of Hebei Province of China (Z2013058), the Human Resources Department of Hebei Province of China (2013–2016 Project), the Educational Commission of Hebei Province of China (ZD2013013). |
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Corresponding Authors:
YANG Zhi-xin, Tel: +86-312-7528236, E-mail: yangzhixin@126.com
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| About author: SHI Wei, Mobile: +86-15933568252, E-mail: 649985324@qq.com |
Cite this article:
SHI Wei, ZHANG Xue-na, JIA Hai-bin, FENG Sheng-dong, YANG Zhi-xin, ZHAO Ou-ya, LI Yu-ling.
2017.
Effective remediation of aged HMW-PAHs polluted agricultural soil by the combination of Fusarium sp. and smooth bromegrass (Bromus inermis Leyss.). Journal of Integrative Agriculture, 16(01): 199-209.
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