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Journal of Integrative Agriculture  2016, Vol. 15 Issue (10): 2353-2362    DOI: 10.1016/S2095-3119(15)61251-5
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Selection and characterisation of lactic acid bacteria isolated from different origins for ensiling Robinia pseudoacacia and Morus alba L. leaves
NI Kui-kui1, YANG Hui-xiao1, HUA Wei2, WANG Yan-ping1, PANG Hui-li1
1 Henan Provincial Key Laboratory of Ion Beam Bio-Engineering, Zhengzhou University, Zhengzhou 450051, P.R.China
2 Henan Sanoterre Bio-Tec Company, Zhengzhou 450000, P.R.China
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Abstract      The objective of this study was to isolate lactic acid bacteria (LAB) strains from different origins and to select the best strains for ensiling Robinia pseudoacacia (RB) and Morus alba L. (MB) leaves. The LAB strains were inoculated into the extracted liquid obtained from RB and MB leaves to evaluate the fermentation products. 11 LAB strains were selected for further experiments based on the highest products of lactic or acetic acid, including 1 strain of Weissella confusa, 2 of Lactobacillus reuteri and 8 of Lactobacillus plantarum. The API 50 CH fermentation experiment indicated that all of the selected 11 LAB strains utilised most of the carbohydrates. All the strains grew at temperatures between 10 and 45°C and at a pH of 3.5 to 4.5; however, L. reuteri F7 and F8 tolerated a pH as low as 3.0. All 11 LAB strains showed antibacterial activity against Listeria monocytogens, Escherichia coil, Salmonella sp. and Acetobacter pasteurianus; however, after excluding the effect of organic acids, only F7 and F8 still exhibited antibacterial activity. The present study indicated that the selected 11 LAB strains could be used to prepare silages of RB and MB leaves, especially L. reuteri F7 and F8.
Keywords:  lactic acid bacteria        Morus alba L.        Robinia pseudoacacia        silage  
Received: 16 September 2015   Accepted:

This work was supported by the Key Technique Project from Henan Province, China (152102110045 and 152102310064).

Corresponding Authors:  WANG Yan-ping, Mobile: +86-13938583206, E-mail:; PANG Hui-li, Tel: +86-371-67897722, E-mail:   
About author:  NI Kui-kui, E-mail:;

Cite this article: 

NI Kui-kui, YANG Hui-xiao, HUA Wei, WANG Yan-ping, PANG Hui-li. 2016. Selection and characterisation of lactic acid bacteria isolated from different origins for ensiling Robinia pseudoacacia and Morus alba L. leaves. Journal of Integrative Agriculture, 15(10): 2353-2362.

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