|
|
|
|
|
| Identification of additional QTLs for flowering time by removing the effect of the maturity gene E1 in soybean |
| LU Si-jia, LI Ying, WANG Jia-lin, NAN Hai-yang, CAO Dong, LI Xiao-ming, SHI Dan-ning, FANG Chao, SHI Xin-yi, YUAN Xiao-hui, Jun Abe, LIU Bao-hui, KONG Fan-jiang |
1、Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of
Sciences, Harbin 150081, P.R.China
2、University of Chinese Academy of Sciences, Beijing 100049, P.R.China
3、State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, P.R.China
4、Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan |
|
|
|
摘要 The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines (RILs) into two sub-populations: one with the E1 allele and another with the e1nl allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two (qFT-B1 and qFT-H) in RILs with the E1 allele and one (qFT-J-2) in the RILs with the e1nl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.
Abstract The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines (RILs) into two sub-populations: one with the E1 allele and another with the e1nl allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two (qFT-B1 and qFT-H) in RILs with the E1 allele and one (qFT-J-2) in the RILs with the e1nl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.
|
|
Received: 13 January 2015
Accepted:
|
| Fund: Supported by the National Natural Science Foundation of China (31430065, 31571686,31201222 and 31371643), the Open Foundation of the Key Laboratory of Soybean Molecular Design Breeding, the “Hundred Talents” Program of the CAAS the Strategic Action Plan for Science and Technology Innovation of the Chinese Academy of Sciences (XDA08030108), the Natural Science Foundation of Heilongjiang Province, China (ZD201001,JC201313), the Research and Development of Applied Technology Project, Harbin, China (2014RFQYJ055), the Scientific Research Foundation for Returned Chinese Scholars of Heilongjiang Province, China (LC201417), the Science Foundation for Creative Research Talents of Harbin Science and Technology Bureau, China (2014RFQYJ046). |
|
Corresponding Authors:
KONG Fan-jiang, Tel/Fax: +86-451-86691226, E-mail: kongfj@iga.ac.cn; LIU Bao-hui, Tel/Fax: +86-451-86685735, E-mail: liubh@iga.ac.cn
E-mail: kongfj@iga.ac.cn;liubh@iga.ac.cn
|
| About author: LU Si-jia, E-mail: jmslsj0310@163.com;* These authors contributed equally to this study. |
Cite this article:
LU Si-jia, LI Ying, WANG Jia-lin, NAN Hai-yang, CAO Dong, LI Xiao-ming, SHI Dan-ning, FANG Chao, SHI Xin-yi, YUAN Xiao-hui, Jun Abe, LIU Bao-hui, KONG Fan-jiang.
2016.
Identification of additional QTLs for flowering time by removing the effect of the maturity gene E1 in soybean. Journal of Integrative Agriculture, 15(1): 42-49.
|
| Bachlava E, Dewey R E, Burton J W, Cardinal A J. 2009.Mapping and comparison of quantitative trait loci for oleicacid seed content in two segregating soybean populations.Crop Science, 49, 433-442Bernard R. 1971. Two major genes for time of flowering andmaturity in soybeans. Crop Science, 11, 242-244Bonato E R, Vello N A. 1999. E6, a dominant gene conditioningearly flowering and maturity in soybeans. Genetics and Molecular Biology, 22, 229-232Buzzell R. 1971. Inheritance of a soybean flowering responseto fluorescent-daylength conditions. Canadian Journal ofGenetics and Cytology, 13, 703-707Buzzell R, Voldeng H. 1980. Inheritance of insensitivity to longdaylength. Soybean Genetics Newsletter, 7, 26-29Cober E R, Morrison M J. 2010. Regulation of seed yield andagronomic characters by photoperiod sensitivity and growthhabit genes in soybean. Theoretical and Applied Genetics,120, 1005-1012Cober E R, Voldeng H D. 2001. A new soybean maturity andphotoperiod-sensitivity locus linked to E1 and T. CropScience, 41, 698-701Fehr W, Caviness C, Burmood D, Pennington J. 1971. Stageof development descriptions for soybeans, Glycine max (L.)Merrill. Crop Science, 11, 929-931Gai J Y, Wang Y J, Wu X L, Chen S Y. 2007. A comparativestudy on segregation analysis and QTL mapping ofquantitative traits in plants-with a case in soybean. Frontiersof Agriculture in China, 1, 1-7Kong F J, Liu B H, Xia Z J, Sato S, Kim B M, Watanabe S,Yamada T, Tabata S, Kanazawa A, Harada K, Abe J. 2010.Two coordinately regulated homologs of FLOWERINGLOCUS T are involved in the control of photoperiodicflowering in soybean. Plant Physiology, 154, 1220-1231Kong F J, Nan H Y, Cao D, Li Y, Wu F F, Wang J L, Lu S J,Yuan X H, Cober E R, Abe J, Liu B H. 2014. A new dominantgene E9 conditions early flowering and maturity in soybean.Crop Science, 54, 1-7Liu B H, Fujita T, Yan Z H, Sakamoto S, Xu D H, Abe J. 2007.QTL mapping of domestication-related traits in soybean(Glycine max). Annals of Botany, 100, 1027-1038Liu B H, Kanazawa A, Matsumura H, Takahashi R, Harada K, AbeJ. 2008. Genetic redundancy in soybean photoresponsesassociated with duplication of the phytochrome A gene.Genetics, 180, 995-1007Liu B H, Watanabe S, Uchiyama T, Kong F J, Kanazawa A, XiaZ J, Nagamatsu A, Arai M, Yamada T, Kitamura K, MasutaC, Harada K, Abe J. 2010. The soybean stem growth habitgene Dt1 is an ortholog of Arabidopsis TERMINAL FLOWER1. Plant Physiology, 153, 198-210McBlain B, Bernard R. 1987. A new gene affecting the time offlowering and maturity in soybeans. Journal of Heredity,78, 160-162Nan H Y, Cao D, Zhang D Y, Li Y, Lu S J, Tang L L, Yuan X H,Liu B H, Kong F J. 2014. GmFT2a and GmFT5a redundantlyand differentially regulate flowering through interaction withand upregulation of the bZIP transcription factor GmFDL19in soybean. PLOS ONE, 9, e97669.Qi X P, Li M W, Xie M, Liu X, Ni M, Shao G H, Song C, Yim A KY, Tao Y, Wong F L, Isobe S, Wong C F, Wong K S, Xu C,Li C, Wang Y, Guan R, Sun F, Fan G, Xiao Z, et al. 2014.Identification of a novel salt tolerance gene in wild soybeanby whole-genome sequencing. Nature Communications,5, 4340.Ray J D, Hinson K, Mankono J, Malo M F. 1995. Geneticcontrol of a long-juvenile trait in soybean. Crop Science,35, 1001-1006Song Q J, Marek L F, Shoemaker R C, Lark K G, ConcibidoV C, Delannay X, Specht J E, Cregan P B. 2004. A newintegrated genetic linkage map of the soybean. Theoreticaland Applied Genetics, 109, 122-128Tasma I M, Lorenzen L L, Green D E, Shoemaker R C. 2001.Mapping genetic loci for flowering time, maturity, andphotoperiod insensitivity in soybean. Molecular Breeding,8, 25-35Van Ooijen J. 2004. MapQTL®5. Software for the mapping ofquantitative trait loci in experimental populations. KyazmaBV, Wageningen.Voorrips R. 2002. MapChart: Software for the graphicalpresentation of linkage maps and QTLs. Journal of Heredity,93, 77-78Watanabe S, Hideshima R, Xia Z, Tsubokura Y, Sato S,Nakamoto Y, Yamanaka N, Takahashi R, Ishimoto M,Anai T, Tabata S, Harada K. 2009. Map-based cloning ofthe gene associated with the soybean maturity locus E3.Genetics, 182, 1251-1262Watanabe S, Xia Z, Hideshima R, Tsubokura Y, Sato S,Yamanaka N, Takahashi R, Anai T, Tabata Satoshi,Kitamura K, Harada K. 2011. A map-based cloning strategyemploying a residual heterozygous line reveals that theGIGANTEA gene is involved in soybean maturity andflowering. Genetics, 188, 395-407Xia Z J, Watanabe S, Yamada T, Tsubokura Y, Nakashima H,Zhai H, Anai T, Sato S, Yamazaki T, Lü S X, Wu H, TabataS, Harada K. 2012. Positional cloning and characterizationreveal the molecular basis for soybean maturity locus E1that regulates photoperiodic flowering. Proceedings ofthe National Academy of Sciences of the United States ofAmerica, 109, E2155-E2164.Yamanaka N, Ninomiya S, Hoshi M, Tsubokura Y, Yano M,Nagamura Y, Sasaki T, Harada K. 2001. An informativelinkage map of soybean reveals QTLs for flowering time,leaflet morphology and regions of segregation distortion.DNA Research, 8, 61-72Yang J, Hu C C, Hu H, Yu R D, Xia Z, Ye X Z, Zhu J.2008. QTLNetwork: Mapping and visualizing geneticarchitecture of complex traits in experimental populations.Bioinformatics, 24, 721-723 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
