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Journal of Integrative Agriculture  2021, Vol. 20 Issue (10): 2781-2796    DOI: 10.1016/S2095-3119(20)63454-2
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
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Increased ammonification, nitrogenase, soil respiration and microbial biomass N in the rhizosphere of rice plants inoculated with rhizobacteria
ZHANG Jun-hua1*, HUANG Jing1*, Sajid HUSSAIN1, ZHU Lian-feng1, CAO Xiao-chuang1, ZHU Chun-quan1, JIN Qian-yu1, ZHANG Hui2
1 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China
2 Agricultural Resources and Environment Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China
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Azospirillum brasilensePseudomonas fluorescens是应用广泛的植物根际促生菌。目前Azospirillum brasilensePseudomonas fluorescens对稻田土壤氮循环和水稻生长发育的影响尚不清楚。本研究通过两年田间试验(2016-2017)解析了Azospirillum brasilensePseudomonas fluorescens对水稻根际土壤氮素转化和供氮能力的影响,明确了Azospirillum brasilensePseudomonas fluorescens在稻田肥料减施增效中的作用。微生物接种包括4个处理,分别为生理盐水接种(对照,M0),水稻幼苗接种Azospirillum brasilense(Mb),水稻幼苗接种Pseudomonas fluorescens(Mp),水稻幼苗接种Azospirillum brasilensePseudomonas fluorescens的混合物(Mbp)。氮肥施用水平包括4个处理,分别为0 kg N hm-2(N0)),90 kg N hm-2(N90),180 kg N hm-2(N180),270 kg N hm-2 (N270)。结果表明,与M0相比,Mbp与Mp处理显著增强了水稻根际土壤氨化作用强度,高氮条件下提升作用更显著。与M0相比,Mbp与Mb处理显著增强了水稻根际土壤固氮酶活性,低氮条件下提升作用更显著。接种用的Azospirillum brasilensePseudomonas fluorescens不参与水稻根际土壤的硝化和反硝化过程。根际促生菌与氮肥的交互作用对土壤呼吸速率与微生物量氮有显著影响。在Mbp处理中,N90、N180、N270处理的土壤供氮能力与水稻产量无显著差异。水稻幼苗接种Azospirillum brasilensePseudomonas fluorescens的混合物,可将该地区氮肥施用量降至90 kg N hm-2

Azospirillum brasilense and Pseudomonas fluorescens are well-known plant growth promoting rhizobacteria.  However, the effects of A. brasilense and P. fluorescens on the N cycles in the paddy field and rice plant growth are little known.  This study investigated whether and how A. brasilense and P. fluorescens contribute to the N transformations and N supply capacities in the rhizosphere, and clarified the effects of A. brasilense and P. fluorescens on the N application rate in rice cultivation.  Inoculations with A. brasilense and P. fluorescens coupled with N application rate trials were conducted in the paddy field in 2016 and 2017.  The inoculations of rice seedlings included four treatments: sterile saline solution (M0), A. brasilense (Mb), P. fluorescens (Mp), and co-inoculation with a mixture of A. brasilense and P. fluorescens (Mbp).  The N application rate included four levels: 0 kg N ha–1 (N0), 90 kg N ha–1 (N90), 180 kg N ha–1 (N180), and 270 kg N ha–1 (N270).  The results indicated that the Mbp and Mp treatments significantly enhanced the ammonification activities in the rhizosphere compared with the M0 treatment, especially for higher N applications, while the Mbp and Mb treatments greatly enhanced the nitrogenase activities in the rhizosphere compared with the M0 treatments, especially for lower N applications.  Azospirillum brasilense and P. fluorescens did not participate in the nitrification processes or the denitrification processes in the soil.  The soil respiration rate and microbial biomass N were greatly affected by the interactions between the rhizobacteria inoculations and the N fertilizer applications.  In the Mbp treatment, N supply capacities and rice grain yields showed no significant differences among the N90, N180, and N270 applications.  The N application rate in the study region can be reduced to 90 kg N ha–1 for  rice seedlings co-inoculated with a mixture of A. brasilense and P. fluorescens.
Keywords:   N transformation        paddy soil        plant growth promoting rhizobacteria        rice productivity  
Received: 08 April 2020   Accepted:
Fund: This study was financially supported by the National Key Research and Development Program of China (2016YFD0200801, 2016YFD0200805), the National Natural Science Foundation of China (31872857), the Foundation of State Key Laboratory of Rice Biology, China National Rice Research Institute (2017ZZKT10404), and the Zhejiang Provincial Natural Science Foundation of China (LY16C130007).
Corresponding Authors:  Correspondence ZHANG Hui, E-mail:; JIN Qian-yu, E-mail:    
About author:  ZHANG Jun-hua, E-mail:; HUANG Jing, E-mail:; * These authors contributed equally to this study.

Cite this article: 

ZHANG Jun-hua, HUANG Jing, Sajid HUSSAIN, ZHU Lian-feng, CAO Xiao-chuang, ZHU Chun-quan, JIN Qian-yu, ZHANG Hui. 2021. Increased ammonification, nitrogenase, soil respiration and microbial biomass N in the rhizosphere of rice plants inoculated with rhizobacteria. Journal of Integrative Agriculture, 20(10): 2781-2796.

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