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Journal of Integrative Agriculture  2022, Vol. 21 Issue (3): 697-709    DOI: 10.1016/S2095-3119(21)63630-4
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
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Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways
TANG Qiong1, ZHENG Xiao-dong1, GUO Jun2, YU Ting1
1 College of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China
2 State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China
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摘要  

虽然SlPti5基因在茄属植物和拟南芥病原体感染中已表现出对相关拮抗基因的关键调控作用,但是关于其对番茄植株生长和抗病过程中ROS系统和激素途径的调控响应机制的研究尚不全面。本研究利用基于病毒诱导的基因沉默(VIGS)系统研究了SlPti5基因在番茄对灰霉防御反应中的作用。表达谱分析表明,SlPti5基因在灰霉病感染后被显著诱导,在番茄叶片和果实中表达量较高。沉默抑制了番茄植株的早期生长,减弱了植株的抗病性,促进了活性氧(ROS)的爆发,影响了参与活性氧清除系统相关基因的表达以及降低了乙烯/茉莉酸信号和茉莉酸介导的信号通路以及调控一些关键的PR蛋白基因的表达来刺激番茄植株对灰霉感染的免疫应答。




Abstract  While SlPti5 has been shown to play a crucial role in the regulation of antagonistic genes in Solanum lycopersicum and Arabidopsis against pathogen infection, there have been no comprehensive studies on the effects of SlPti5 on the regulatory response mechanism of reactive oxygen species (ROS) system and hormone pathways during growth and disease resistance of tomato plants.  Here, we investigated the function of SlPti5 in the defense response of tomato against Botrytis cinerea utilizing a virus-induced gene silencing (VIGS)-based system.  Expression profile analysis showed that SlPti5 was significantly induced upon B. cinerea infection, with high expression levels in the leaves and fruit of tomato.  VIGS-based silencing of SlPti5 inhibited early vegetative growth, increased the plant’s susceptibility to infection, promoted the development of ROS, affected the expression of genes involved in the ROS scavenging system, and attenuated the expression of genes associated with pathogenesis and the ethylene/jasmonic acid signaling pathways.  In sum, our data demonstrated that SlPti5 stimulates the immune response of tomato plant to Botrytis cinerea infection by involving the ethylene (ET)- and jasmonic acid (JA)-mediated pathways and modulating the expression of some key pathogenesis-related (PR) genes.
Keywords:  Tomato (Solanum lycopersicum)        Botrytis cinerea        SlPti5        Immune response        ET - and JA - mediated signaling pathways        Pathogenesis-related proteins  
Received: 06 August 2020   Accepted: 15 January 2021
Fund: This research was supported by the National Key Technology R&D Program of China (2016YFD0401201), the National Natural Science Foundation of China (31801602 and 31571897), the Project of Tianjin Education Commission Scientific Research Plan, China (2018KJ094) and the National Science and Technology Major Project of China (2018ZX10101003-002-004).
Corresponding Authors:  TANG Qiong, E-mail: tangqiong1104@zju.edu.cn;    
About author:  TANG Qiong, E-mail: tangqiong1104@zju.edu.cn; Correspondence YU Ting, Tel/Fax: +86-571-88982191, E-mail: yuting@zju.edu.cn; GUO Jun, Tel/Fax: +022-60912486, E-mail: guojun@tust.edu.cn;

Cite this article: 

TANG Qiong, ZHENG Xiao-dong, GUO Jun, YU Ting. 2022. Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways. Journal of Integrative Agriculture, 21(3): 697-709.

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