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| Genome-wide identification, expression and functional analysis of sugar transporters in sorghum (Sorghum bicolor L.) |
XIAO Qian-lin1*, LI Zhen1*, WANG Ya-yun2, HOU Xian-bin3, WEI Xi-mei1, ZHAO Xiao1, HUANG Lei1, GUO Yan-jun4, LIU Zhi-zhai1
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1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, P.R.China
2 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, P.R.China
3 College of Agriculture and Food Engineering, Baise University, Baise 533000, P.R.China
4 College of Animal Science and Technology, Southwest University, Chongqing 400715, P.R.China
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摘要 糖转运蛋白在植物渗透调节、信号途径以及植物的生长发育过程中具有重要的作用。然而，目前高粱糖转运蛋白（Sorghum Sugar Transporter，SST）的功能研究却相对较少。本研究通过BLASTP在全基因组鉴定得到98个SST。分析结果显示，这98个SST被划分成3个家族，其中6个被划分为蔗糖转运蛋白家族（sucrose transporters，SUT），23个被划分为SWEET蛋白家族（sugars will eventually be exported transporters，SWEET），69个被划分为单糖转运蛋白家族（monosaccharide transporters，MST）。并且这69个高粱MST可进一步分为7个亚家族，其中24个蛋白属于sugar transporter protein（STP），23个属于polyol/monosaccharide transporter（PLT），2个属于vacuolar glucose transporter（VGT），4个属于inositol transporter（INT），3个属于plastidic glucose transporter/suppressor of G protein beta1（pGlcT/SBG1），5个属于tonoplastic monosaccharide transporter（TMT），8个属于early response to dehydration (ERD6)-like（ERD）。研究结果还发现，SST的编码基因在染色体上随机分布，但却呈现成簇分布的特性，SWEET、ERD、STP和PLT的27个编码基因聚集形成8个串联重复区，其中22个编码基因形成了11对旁系同源基因，占SST编码基因的22.4%。此外，SST家族具有相似的保守结构域，但其保守基序与跨膜结构域（TMH）的特征却有所不同。进一步的分析结果显示，SST编码基因表现出明显的组织特异性；有7个SST主要分布在细胞膜以及膜细胞器上；而选取的14个SST则均能在酵母中转运不同类型的单糖。通过以上研究，我们揭示了SST的序列特征和蛋白的初步功能，研究结果为解析SST在高粱的糖转运及糖信号通路中的作用奠定了基础。
Abstract
Sugar transporters are essential for osmotic process regulation, various signaling pathways and plant growth and development. Currently, few studies are available on the function of sugar transporters in sorghum (Sorghum bicolor L.). In this study, we performed a genome-wide survey of sugar transporters in sorghum. In total, 98 sorghum sugar transporters (SSTs) were identified via BLASTP. These SSTs were classified into three families based on the phylogenetic and conserved domain analysis, including six sucrose transporters (SUTs), 23 sugars will eventually be exported transporters (SWEETs), and 69 monosaccharide transporters (MSTs). The sorghum MSTs were further divided into seven subfamilies, including 24 STPs, 23 PLTs, two VGTs, four INTs, three pGlcT/SBG1s, five TMTs, and eight ERDs. Chromosomal localization of the SST genes showed that they were randomly distributed on 10 chromosomes, and substantial clustering was evident on the specific chromosomes. Twenty-seven SST genes from the families of SWEET, ERD, STP, and PLT were found to cluster in eight tandem repeat event regions. In total, 22 SSTs comprising 11 paralogous pairs and accounting for 22.4% of all the genes were located on the duplicated blocks. The different subfamilies of SST proteins possessed the same conserved domain, but there were some differences in features of the motif and transmembrane helices (TMH). The publicly-accessible RNA-sequencing data and real-time PCR revealed that the SST genes exhibited distinctive tissue specific patterns. Functional studies showed that seven SSTs were mainly located on the cell membrane and membrane organelles, and 14 of the SSTs could transport different types of monosaccharides in yeast. These findings will help us to further elucidate their roles in the sorghum sugar transport and sugar signaling pathways.
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Received: 10 March 2021
Accepted: 07 May 2021
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| Fund: This work was supported by the National Natural Science Foundation of China (32001607) and Fundamental Research Funds for the Central Universities of Southwest University (No: SWU118087). |
| About author: Correspondence XIAO Qian-lin, Tel: +86-23-68251410, E-mail: xiaoql1853@swu.edu.cn; LIU Zhi-zhai, Tel: +86-23-68251410, E-mail: liu003@swu.edu.cn
* These authors contributed equally to this study. |
Cite this article:
XIAO Qian-lin, LI Zhen, WANG Ya-yun, HOU Xian-bin, WEI Xi-mei, ZHAO Xiao, HUANG Lei, GUO Yan-jun, LIU Zhi-zhai.
2022.
Genome-wide identification, expression and functional analysis of sugar transporters in sorghum (Sorghum bicolor L.) . Journal of Integrative Agriculture, 21(10): 2848-2864.
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