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Special Issue:
动物科学合辑Animal Science
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| Regulation of bone phosphorus retention and bone development possibly by BMP and MAPK signaling pathways in broilers |
| LIAO Xiu-dong1*, CAO Su-mei1, 3*, LI Ting-ting2, SHAO Yu-xin1, ZHANG Li-yang1, LU Lin1, ZHANG Ri-jun3, HOU Shui-sheng1, LUO Xu-gang2 |
1 Mineral Nutrition Research Division, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou 225000, P.R.China
3 Laboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China |
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摘要 骨形态蛋白（bone morphogenetic protein，BMP）和促分裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）信号通路在调控骨骼形成和发育中扮演着重要角色。然而，目前关于肉鸡饲粮不同非植酸磷水平（non-phytate phosphorus，NPP）对这些信号通路及其与骨磷沉积和骨骼发育相关性的影响尚不清楚。因此，本试验旨在研究饲粮添加磷对肉鸡BMP和MAPK信号通路及其与骨磷沉积和骨骼发育相关性的影响。将800只1日龄AA肉公鸡按体重随机分成5个处理组，每个处理组8个重复。5个处理组饲粮的NPP水平分别为：1-21日龄：0.15、0.25、0.35、0.45和0.55%，22-42日龄：0.15、0.22、0.29、0.36和0.43%。试验结果表明，随着饲粮NPP水平的增加，14和28日龄肉鸡胫骨细胞外信号调节激酶1（Extracellular Signal-Regulated Kinase 1，ERK1）的mRNA表达、14日龄肉鸡胫骨磷酸化ERK1及28和42日龄肉鸡胫骨BMP2的蛋白表达线性降低（P<0.04），而42日龄肉鸡胫骨c-Jun氨基末端激酶1（c-Jun N-terminal kinase 1，JNK1）的mRNA表达线性增加（P<0.02）。在14日龄，肉鸡胫骨灰分总磷沉积量、骨矿物质含量、骨密度、骨强度及胫骨灰分与ERK1和JNK1 mRNA表达及磷酸化ERK1呈显著负相关（r=-0.726~-0.359，P<0.05），而胫骨碱性磷酸酶活性和骨钙素含量与ERK1 mRNA表达及磷酸化ERK1呈显著正相关（r=0.405~0.665，P<0.01）。在28日龄，肉鸡的胫骨灰分总磷沉积量、骨矿物质含量、骨密度、骨强度及胫骨灰分与ERK1 mRNA表达和BMP2蛋白表达呈显著负相关（r=-0.518~-0.370，P<0.05），而与胫骨碱性磷酸酶呈显著正相关（r=0.382~0.648，P<0.05）。结果表明，ERK1和JNK1 mRNA表达、BMP2蛋白表达及磷酸化ERK1与胫骨灰分总磷沉积量、骨矿物质含量、骨密度、骨强度及胫骨灰分呈显著负相关，但是与胫骨碱性磷酸酶活性和骨钙素呈显著正相关，表明肉鸡骨磷沉积和骨骼发育受BMP和MAPK信号通路的调节。本研究揭示了肉鸡骨磷沉积和骨骼发育受BMP和MAPK信号通路调节的机制。
Abstract
The bone morphogenetic protein (BMP) and mitogen-activated protein kinase (MAPK) signaling pathways play an important role in regulation of bone formation and development, however, it remains unclear that the effect of dietary different levels of non-phytate phosphorus (NPP) on these signaling pathways and their correlations with bone phosphorus (P) retention and bone development in broilers. Therefore, this experiment was conducted to investigate the effect of dietary P supplementation on BMP and MAPK signaling pathways and their correlations with bone P retention and bone development in broilers. A total of 800 one-day-old Arbor Acres male broilers were randomly allotted to 1 of 5 treatments with 8 replicates in a completely randomized design. The 5 treatments of dietary NPP levels were 0.15, 0.25, 0.35, 0.45 and 0.55% or 0.15, 0.22, 0.29, 0.36 and 0.43% for broilers from 1 to 21 days of age or 22 to 42 days of age, respectively. The results showed that extracellular signal-regulated kinase 1 (ERK1) mRNA expression in the tibia of broilers on days 14 and 28, phosphorylated-ERK1 (p-ERK1) on day 14, and BMP2 protein expression on days 28 and 42 decreased linearly (P<0.04), while c-Jun N-terminal kinase 1 (JNK1) mRNA expression on day 42 increased linearly (P<0.02) with the increase of dietary NPP level. At 14 days of age, total P accumulation in tibia ash (TPTA), bone mineral concentration (BMC), bone mineral density (BMD), bone breaking strength (BBS) and tibia ash were negatively correlated (r=–0.726 to –0.359, P<0.05) with ERK1 and JNK1 mRNA as well as p-ERK1; tibia alkaline phosphatase (ALP) and bone gal protein (BGP) were positively correlated (r=0.405 to 0.665, P<0.01) with ERK1 mRNA and p-ERK1. At 28 days of age, TPTA, BMC, BMD, BBS and tibia ash were negatively correlated (r=–0.518 to –0.370, P<0.05) with ERK1 mRNA and BMP2 protein, while tibia ALP was positively correlated (r=0.382 to 0.648, P<0.05) with them. The results indicated that TPTA, BMC, BMD, BBS or tibia ash had negative correlations, while tibia ALP and BGP had positive correlations with ERK1 and JNK1 mRNAs, BMP2 protein and p-ERK1, suggesting that bone P retention and bone development might be regulated by BMP and MAPK signaling pathways in broiler chickens.
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Received: 09 June 2021
Accepted: 22 September 2021
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| Fund: The present study was supported by the Key Program of the National Natural Science Foundation of China (31630073), the China Agriculture Research System of MOF and MARA (CARS-41), and the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS09). |
| About author: LIAO Xiu-dong, Tel: +86-10-62820866, E-mail: liaoxd56@163.com; Correspondence LUO Xu-gang, E-mail: wlysz@263.net
* These authors contributed equally to this study. |
Cite this article:
LIAO Xiu-dong, CAO Su-mei, LI Ting-ting, SHAO Yu-xin, ZHANG Li-yang, LU Lin, ZHANG Ri-jun, HOU Shui-sheng, LUO Xu-gang.
2022.
Regulation of bone phosphorus retention and bone development possibly by BMP and MAPK signaling pathways in broilers. Journal of Integrative Agriculture, 21(10): 3017-3025.
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