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Journal of Integrative Agriculture  2017, Vol. 16 Issue (11): 2525-2533    DOI: 10.1016/S2095-3119(17)61658-7
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Electrophysiological responses of the rice striped stem borer Chilo suppressalis to volatiles of the trap plant vetiver grass (Vetiveria zizanioides L.)
LU Yan-hui, LIU Kai, ZHENG Xu-song, LÜ Zhong-xian
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
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Abstract  Vetiver grass (Vetiveria zizanioides L.) was previously found to effectively attract female adults of Chilo suppressalis (Walker), an important pest of rice.  To determine the volatile compounds involved in this attraction, electroantennography (EAG) responses to seven synthetic volatiles released from vetiver plants were examined.  Our results indicated that the responses of C. suppressalis adult antennae to the different compounds varied widely.  The compounds elicited strong EAG responses in female antennae were subsequently selected for further EAG response tests, namely, caryophyllene, β-ocimene, linalool and α-pinene.   EAG responses to a combination of these four compounds did not differ significantly from the individual compounds.  However, pair combination tests indicated that 0.01 μg μL–1 linalool and 50 μg μL–1 α-pinene, 50 μg μL–1 caryophyllene and 0.01 μg μL–1 linalool, 0.01 μg μL–1 β-ocimene and 0.01 μg μL–1 linalool, and 0.01 μg μL–1 β-ocimene and 50 μg μL–1 caryophyllene elicited significantly greater EAG responses in 3-day female moths compared to the 1-day female.  These compound combinations and the corresponding ratios are probably playing an important role in attracting female adults of C. suppressalis to the vetiver grass.  
Keywords:  Chilo suppressalis        electroantennography (EAG)        volatiles        vetiver grass        trapping mechanism        attraction  
Received: 23 January 2017   Accepted:

This work was supported by the National Natural Science Foundation of China (31672050), the National Key Research and Development Program of China (2016YFD0200800-04), and the Zhejiang Key Research and Development Program, China (2015C02014).

Corresponding Authors:  Correspondence Lü Zhong-xian, Tel: +86-571-86404077, Fax: +86-571-86404255, E-mail:   
About author:  LU Yan-hui, E-mail:

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