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Journal of Integrative Agriculture  2019, Vol. 18 Issue (1): 1-8    DOI: 10.1016/S2095-3119(17)61876-8
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Identification and characterization of cell cultures with various embryogenic/regenerative potential in cotton based on morphological, cytochemical, and cytogenetical assessment
UO Hui-hui1, WU Jian-fei1, CHEN Cui-xia1, WANG Hong-mei2, ZHAO Yun-lei2, ZHANG Chao-jun2, JIA Yin-hua2, LIU Fang2, NING Tang-yuan3, CHU Zhao-hui1, ZENG Fan-chang
1 State Key Laboratory of Crop Biology, Ministry of Science and Technology/College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
2 State Key Laboratory of Cotton Biology, Ministry of Science and Technology/Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China
3 Key Laboratory of Water Resources and Drought Resistant Germplasm Improvement, Ministry of Agriculture, Tai’an 271018, P.R.China
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Somatic embryogenesis (SE) plays a vital role in genetic transformation and massive propagation of important agronomical and economical crops.  Here, we conducted a systematic assessment of the morphological, cytochemical, and cytogenetical characteristics of six culture strains with various embryogenic/regenerative potential during SE process in cotton.  Results indicated that the six cell culture strains had stable ploidy levels, and did not reveal any relationship between the cytogenetic state and their morphogenetic potential.  Moreover, the six culture strains were compared via double staining with Evans blue and Acetocarmine to efficiently distinguish embryogenic and non-embryogenic cells and determine the embryogenic nature of the calli.  In addition, the kind of auxins added in medium affected not only growth property, color, size of cell clumps but also ploidy level and regeneration ability.  By combining analysis of morphological, cytochemical, and cytogenetical characteristics of the cell cultures, we are able to obtain and maintain homogeneous cell population with high morphogenic and regeneration ability and establish efficient somatic embryogenesis and regeneration system from short-term cell cultures in upland cotton, which highlight the application of biotechnological approaches in crop breeding, and above all, to better understand totipotency of cells in higher plants.
Keywords:  cotton somatic embryogenesis  cotton cell culture strains        cytochemical EB-AC double staining        cytogenetical ploidy stability        embryogenic/regenerative potential, morphology  
Received: 25 November 2017   Accepted: 02 January 2019
Fund: Program of China (2016YFD0100306), the National Natural Science Fundation of China (31401428), the Fok Ying-Tong Education Foundation of China (151024), and the Taishan Scholar Talent Project from China (tsqn20161018).
Corresponding Authors:  Correspondence ZENG Fan-chang, Tel: +86-538-8241828, E-mail:   
About author:  GUO Hui-hui, E-mail:;

Cite this article: 

GUO Hui-hui, WU Jian-fei, CHEN Cui-xia, WANG Hong-mei, ZHAO Yun-lei, ZHANG Chao-jun, JIA Yin-hua, LIU Fang, NING Tang-yuan, CHU Zhao-hui, ZENG Fan-chang. 2019. Identification and characterization of cell cultures with various embryogenic/regenerative potential in cotton based on morphological, cytochemical, and cytogenetical assessment. Journal of Integrative Agriculture, 18(1): 1-8.

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